A temperate phage with cohesive ends induced by mitomycin C treatment of Lactobacillus casei

1998 ◽  
Vol 143 (8) ◽  
pp. 1621-1626 ◽  
Author(s):  
Y. Nakashima ◽  
H. Hasuwa ◽  
Y. Kakita ◽  
K. Murata ◽  
A. Kuroiwa ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Lactobacillus Casei ◽  
Temperate Phage

scholarly journals Moderate Seasonal Dynamics Indicate an Important Role for Lysogeny in the Red Sea

2021 ◽  
Vol 9 (6) ◽  
pp. 1269
Author(s):  
Ruba Abdulrahman Ashy ◽  
Curtis A. Suttle ◽  
Susana Agustí
Keyword(s):  
Surface Water ◽  
Mitomycin C ◽  
Red Sea ◽  
Environmental Changes ◽  
Viral Infections ◽  
High Salinity ◽  
High Surface ◽  
Temperate Phage ◽  
Viral Dynamics ◽  
Second Year

Viruses are the most abundant microorganisms in marine environments and viral infections can be either lytic (virulent) or lysogenic (temperate phage) within the host cell. The aim of this study was to quantify viral dynamics (abundance and infection) in the coastal Red Sea, a narrow oligotrophic basin with high surface water temperatures (22–32 °C degrees), high salinity (37.5–41) and continuous high insolation, thus making it a stable and relatively unexplored environment. We quantified viral and environmental changes in the Red Sea (two years) and the occurrence of lysogenic bacteria (induced by mitomycin C) on the second year. Water temperatures ranged from 24.0 to 32.5 °C, and total viral and bacterial abundances ranged from 1.5 to 8.7 × 106 viruses mL−1 and 1.9 to 3.2 × 105 bacteria mL−1, respectively. On average, 12.24% ± 4.8 (SE) of the prophage bacteria could be induced by mitomycin C, with the highest percentage of 55.8% observed in January 2018 when bacterial abundances were low; whereas no induction was measurable in spring when bacterial abundances were highest. Thus, despite the fact that the Red Sea might be perceived as stable, warm and saline, relatively modest changes in seasonal conditions were associated with large swings in the prevalence of lysogeny.

LYSOGENY IN A STRAIN OF XANTHOMONAS GAMPESTRIS

1963 ◽  
Vol 9 (6) ◽  
pp. 821-828 ◽  
Author(s):  
M. D. Sutton ◽  
C. Quadling
Keyword(s):  
Electron Microscope ◽  
Host Range ◽  
Mitomycin C ◽  
Xanthomonas Campestris ◽  
Temperate Phage ◽  
Nutrient Broth ◽  
Bacterial Strains ◽  
Phage Development ◽  
Before And After

Fourteen bacterial strains representing eight so-called species of phytopathogenic xanthomonads were tested for lysogeny both before and after treatments known to induce phage development in lysogenic bacteria. Only a single temperate phage, of restricted host range, was found. Evidence suggestive of defective lysogeny was found in a number of cases.Strain P165, Xanthomonas campestris, when treated with antibiotic Mitomycin C at a concentration of 0.1,ug/ml in nutrient broth, produced phage particles active on strain P12S, X. campestris. No other sensitive strains were found among the 81 tested, representing 22 species of Xanthomonas. This phage (P165/P125) produced small turbid plaques, 0.6 mm to 0.8 mm in diameter, with indefinite margins. Evidence for the lysogenization of P125 strain by this phage was found. Clear plaques were sometimes found in lawns of lysogenized cells of P12S. These were presumably derived from phage mutants with increased virulence. When examined under the electron microscope, the phage particles were semispherical, approximately 65 m/x in diameter with rudimentary tails.

scholarly journals Effect of Mitomycin C on Lactobacillus casei-Phage J1 System

1972 ◽  
Vol 46 (1) ◽  
pp. 35-44
Author(s):  
Akira MURATA ◽  
Masanobu TSUJI ◽  
Eiichi SOEDA ◽  
Rinjiro SARUNO ◽  
Toshizo SAKURAI
Keyword(s):  
Mitomycin C ◽  
Lactobacillus Casei

scholarly journals Genome Sequence of vB_AbaS_TRS1, a Viable Prophage Isolated from Acinetobacter baumannii Strain A118

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Dann Turner ◽  
Matthew E. Wand ◽  
J. Mark Sutton ◽  
Daniela Centron ◽  
Andrew M. Kropinski ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Mitomycin C ◽  
Genome Sequence ◽  
Temperate Phage ◽  
Coding Sequences

A novel temperate phage, vB_AbaS_TRS1, was isolated from cultures of Acinetobacter baumannii strain A118 that had been exposed to mitomycin C. Phage TRS1 belongs to the Siphoviridae family of bacteriophages and encapsulates a 40,749-bp genome encoding 70 coding sequences and a single tRNA.

Mitomycin C induction of a temperate phage in Pseudomonas aeruginosa

1968 ◽  
Vol 14 (6) ◽  
pp. 667-673 ◽  
Author(s):  
T. Yamamoto ◽  
C. T. Chow
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Dna Synthesis ◽  
Mitomycin C ◽  
Buoyant Density ◽  
Cell Lysis ◽  
Temperate Phage ◽  
Phage Tail ◽  
Phage Dna ◽  
Icosahedral Head

After addition of mitomycin C to Pseudomonas aeruginosa strain MAC-264 cells, DNA synthesis stops but resumes again after 45 min. This resumption in DNA synthesis is due to the induction of a temperate phage. Early after induction, both RNA and protein syntheses continue but later on little or no net synthesis of these macromolecules occurs. Cell lysis occurs at 90 to 105 min after addition of mitomycin C. Analysis of the lysates indicates that a DNA phage with an icosahedral head about 55 mμ in diameter with a short wedge-shaped tail is produced. This phage [Formula: see text] has a buoyant density of 1.468 g/ml in CsCl. The guanine + cytosine content of the phage DNA determined by its buoyant density in CsCl is 46% compared to 68% for the host DNA.Some phage tail-like structures which have a buoyant density of 1.305 g/ml in CsCl are also induced at the same time.

1570: Combined Mitomycin C and Hyperthermia (SYNERGO) for Patients with Superficial Bladder Cancer (SBC) after Bacillus Calmette-Guerin (BCG) Failure

2007 ◽  
Vol 177 (4S) ◽  
pp. 519-519
Author(s):  
Ofer Nativ ◽  
Renzo Colombo ◽  
Dov Engelstein ◽  
Ofer N. Gofrit ◽  
Thomas Akkad ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Mitomycin C ◽  
Superficial Bladder Cancer ◽  
Bacillus Calmette Guerin ◽  
Bcg Failure

832: Optimized Intravesical Mitomycin C Treatment for Superficial Bladder Cancer: Long-Term Follow-Up

2006 ◽  
Vol 175 (4S) ◽  
pp. 268-269 ◽  
Author(s):  
Jessie L. Au ◽  
Robert A. Badalament ◽  
M. Guillaume Wientjes ◽  
Donn C. Young ◽  
Tong Shen ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Mitomycin C ◽  
Superficial Bladder Cancer ◽  
Long Term Follow Up

Erste Ergebnisse – Die gedeckte Sklerotomie unter primärer Anwendung von Mitomycin C 0,02%

2008 ◽  
Vol 225 (S 04) ◽  
Author(s):  
E Karioris ◽  
C Wirbelauer ◽  
H Häberle ◽  
DT Pham
Keyword(s):  
Mitomycin C ◽  
Erste Ergebnisse

Lokale Applikation von Mitomycin C bei Atemwegsstenosen

Pneumologie ◽  
2009 ◽  
Vol 63 (S 01) ◽  
Author(s):  
PC Bauer ◽  
U Sommerwerck ◽  
J Hagmeyer ◽  
F Bonella ◽  
U Costabel
Keyword(s):  
Mitomycin C
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