scholarly journals Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease

1998 ◽  
Vol 143 (5) ◽  
pp. 915-930 ◽  
Author(s):  
M. Sheppard ◽  
W. Werner ◽  
E. Tsatas ◽  
R. McCoy ◽  
S. Prowse ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Protective Immunity ◽  
Infectious Bursal Disease ◽  
Fowl Adenovirus ◽  
Bursal Disease ◽  
Adenovirus Recombinant

scholarly journals Experimental co-infection of variant infectious bursal disease virus and fowl adenovirus serotype 4 increases mortality and reduces immune response in chickens

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
A-hui Xu ◽  
Lu Sun ◽  
Kai-hang Tu ◽  
Qing-yuan Teng ◽  
Jia Xue ◽  
...  
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Clinical Symptoms ◽  
Interaction Mechanism ◽  
Infectious Bursal Disease ◽  
Adenovirus Serotype ◽  
Fowl Adenovirus ◽  
Bursal Disease ◽  
Fowl Adenovirus Serotype 4

AbstractInfectious bursal disease virus (IBDV) and fowl adenovirus serotype 4 (FAdV-4) cause infectious bursal disease (IBD) and hydropericardium-hepatitis syndrome, respectively. Recently, studies have reported co-infections of poultry with IBDV and FAdV-4, which is an important problem in the poultry industry. Here, the variant IBDV strain ZD-2018-1 and FAdV-4 isolate HB1501 were used to assess the pathogenicity of co-infection in 1-day-old specific pathogen-free (SPF) chickens. Compared with chickens infected with only FAdV-4, those coinfected with IBDV and FAdV-4 showed enhanced clinical symptoms, higher mortality, more severe tissue lesions, and higher biochemical index levels. Furthermore, the expression of interleukin (IL)-6, IL-1β, and interferon-γ mRNAs in the IBDV-FAdV-4 coinfected chickens was delayed, and the antibody response levels were significantly lower in those birds compared with the FAdV-4-infected chickens. These results indicate that co-infection with variant IBDV ZD-2018-1 and FAdV-4 HB1501 could significantly promote the pathogenicity of FAdV-4 and reduce the immune response in chickens. This study provides the foundation for further investigation of the interaction mechanism in IBDV and FAdV-4 co-infection.

scholarly journals Dual-Viral Vector Approach Induced Strong and Long-Lasting Protective Immunity against Very Virulent Infectious Bursal Disease Virus

Virology ◽  
2000 ◽  
Vol 269 (2) ◽  
pp. 257-267 ◽  
Author(s):  
Kenji Tsukamoto ◽  
Takanori Sato ◽  
Shuji Saito ◽  
Nobuhiko Tanimura ◽  
Naoki Hamazaki ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Protective Immunity ◽  
Viral Vector ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Vector Approach

scholarly journals An unusual outbreak of inclusion body hepatitis on a broiler chicken farm: a case report

2017 ◽  
Vol 62 (No. 11) ◽  
pp. 631-635
Author(s):  
V. Revajova ◽  
R. Herich ◽  
V. Seman ◽  
M. Levkut Jr ◽  
M. Levkutova ◽  
...  
Keyword(s):  
Antibody Response ◽  
Disease Virus ◽  
Inclusion Body ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Antibody Detection ◽  
Chicken Anaemia Virus ◽  
Fowl Adenovirus ◽  
Inclusion Body Hepatitis ◽  
Bursal Disease

This study investigated an outbreak of inclusion body hepatitis in ROSS 308 hybrid broiler type chickens between 19 and 25 days of fattening. For this purpose, clinical observation, ELISA fowl adenovirus and chicken anaemia virus antibody detection in serum at 21 and 42 days, mortality evaluation, epidemiological analysis, histology and genetic identification were performed. The six flocks of the farm consisted of 90,000 chickens. Only one flock of 15,000 chickens was affected on this farm. At 19 days of age, ill chickens showed clinical signs of depression, anorexia, somnolence, ruffled feathers, anaemic comb and wattles and occasionally nervous signs. Based on ELISA titres, the antibody response to fowl adenovirus increased greatly from 21 to 42 days. The antibody response to vaccination against infectious bursal disease virus and chicken anaemia virus was at the expected level in all broiler flocks. Necropsy showed diffuse petechial and ecchymotic haemorrhages in skeletal muscles, liver, pancreas, kidney, together with hepatomegaly, splenomegaly and catarrhal enteritis. Histologically, fatty liver degeneration, multifocal liver necrosis and intranuclear inclusions in hepatocytes, as well as focal necrosis in pancreas and spleen parenchyma were seen. The DNA of AAV-1 (avian adenovirus group 1) was detected using the PCR method in paraffin-embedded liver samples. The results revealed no association of inclusion body hepatitis with infectious bursal disease virus or chicken anaemia virus infection, and suggested primary disease. However, the involvement of only one chicken flock on the farm remains unexplained.

scholarly journals INDUCTION OF PROTECTIVE IMMUNITY IN CHICKENS IMMUNISED WITH PLASMID DNA ENCODING INFECTIOUS BURSAL DISEASE VIRUS ANTIGENS

1999 ◽  
Vol 47 (4) ◽  
pp. 481-492 ◽  
Author(s):  
Nadja Fodor ◽  
S. K. Dube ◽  
I. Fodor ◽  
E. Horváth ◽  
Edith Nagy ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Virus ◽  
Plasmid Dna ◽  
Infectious Bursal Disease Virus ◽  
Protective Immunity ◽  
Infectious Bursal Disease ◽  
Dna Encoding ◽  
New Approach ◽  
Specific Antibodies ◽  
Bursal Disease

Direct DNA inoculations were used to determine the efficacy of gene immunisation of chickens to elicit protective immune responses against infectious bursal disease virus (IBDV). Thevp2 gene of IBDV strains GP40 and D78, and thevp2-vp4-vp3 encoding segment of strain D78 were cloned in an expression vector which consisted of human cytomegalovirus (HCMV) immediate early enhancer and promoter, adenovirus tripartite leader sequences and SV40 polyadenylation signal. For purification of vaccine-quality plasmid DNA fromE. coli, an effective method was developed. Chickens were vaccinated by inoculation of DNA by two routes (intramuscular and intraperitoneal). Two weeks later, chickens were boosted with DNA, and at 2 weeks post-boost, they were challenged with virulent IBDV strain. Low to undetectable levels of IBDV-specific antibodies and no protection were observed with DNA encoding VP2. However, plasmids encoding VP2-VP4-VP3 induced IBDV-specific antibodies and protection in the chickens. DNA immunisation opens a new approach to the development of gene vaccines for chickens against infectious diseases.

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