The nucleotide sequence of the coat protein genes of satsuma dwarf virus and navel orange infectious mottling virus

1998 ◽  
Vol 143 (2) ◽  
pp. 405-412 ◽  
Author(s):  
T. Iwanami ◽  
Y. Kondo ◽  
Y. Makita ◽  
C. Azeyanagi ◽  
H. Ieki
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Dwarf Virus ◽  
Navel Orange ◽  
Satsuma Dwarf Virus

A new virus related to Satsuma dwarf virus: the nucleotide sequence of the 3?-terminal regions of Hyuganatsu virus RNAs 1 and 2

2004 ◽  
Vol 149 (7) ◽  
Author(s):  
T. Ito ◽  
T. Iwanami ◽  
H. Ieki ◽  
K. Shimomura ◽  
S. Shimizu ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Dwarf Virus ◽  
Satsuma Dwarf Virus

scholarly journals Nucleotide Sequence of the 3'-Terminal Region of RNA1 of Satsuma Dwarf Virus.

1996 ◽  
Vol 62 (1) ◽  
pp. 4-10 ◽  
Author(s):  
Toru IWANAMI ◽  
Fumiaki YAMAO ◽  
Takeshi SENO ◽  
Hiroyuki IEKI
Keyword(s):  
Nucleotide Sequence ◽  
Terminal Region ◽  
Dwarf Virus ◽  
Satsuma Dwarf Virus

scholarly journals Nucleotide sequence of the potato leafroll virus coat protein gene

1989 ◽  
Vol 17 (4) ◽  
pp. 1768-1768 ◽  
Author(s):  
B. Prill ◽  
E. Maiss ◽  
U. Timpe ◽  
R. Casper
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Leafroll Virus

Nucleotide Sequence from the Coat Protein Cistron of R17 Bacteriophage RNA

Nature ◽  
1969 ◽  
Vol 223 (5210) ◽  
pp. 1009-1014 ◽  
Author(s):  
J. M. ADAMS ◽  
P. G. N. JEPPESEN ◽  
F. SANGER ◽  
B. G. BARRELL
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein

scholarly journals Nucleotide Sequence of Rice Dwarf Virus Genome Segment 9

1989 ◽  
Vol 70 (5) ◽  
pp. 1297-1300 ◽  
Author(s):  
I. Uyeda ◽  
H. Kudo ◽  
T. Takahashi ◽  
T. Sano ◽  
K. Ohshima ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Virus Genome ◽  
Genome Segment ◽  
Dwarf Virus ◽  
Rice Dwarf Virus

scholarly journals First Report of Raspberry bushy dwarf virus in Rubus multibracteatus from China

Plant Disease ◽  
2003 ◽  
Vol 87 (5) ◽  
pp. 603-603 ◽  
Author(s):  
C. J. Chamberlain ◽  
J. Kraus ◽  
P. D. Kohnen ◽  
C. E. Finn ◽  
R. R. Martin
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Black Raspberry ◽  
First Report ◽  
Raspberry Bushy Dwarf Virus ◽  
Plant Pathol

Raspberry bushy dwarf virus (RBDV), genus Idaeovirus, has been reported in commercial Rubus spp. from North and South America, Europe, Australia, New Zealand, and South Africa. Infection can cause reduced vigor and drupelet abortion leading to crumbly fruit and reduced yields (3,4). In recent years, Rubus germplasm in the form of seed, was obtained on several collection trips to The People's Republic of China to increase the diversity of Rubus spp. in the USDA-ARS National Clonal Germplasm Repository, (Corvallis, OR). Before planting in the field, seedlings were tested for the presence of RBDV, Tomato ringspot virus, and Tobacco streak virus using triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) (antiserum produced by R. R. Martin). One symptomless plant of R. multibracteatus H. Lev. & Vaniot (PI 618457 in USDA-ARS GRIN database), from Guizhou province in China, tested positive for RBDV (RBDV-China). After mechanical transmission on Chenopodium quinoa Willd., this isolate produced typical symptoms of RBDV (3). To determine if RBDV-China was a contaminant during the handling of the plants, or if the source was a seedborne virus, the coat protein gene was sequenced and compared to published sequences of RBDV. RNA was extracted from leaves of R. multibracteatus and subjected to reverse transcription-polymerase chain reaction (RT-PCR) using primers that flank the coat protein gene. Products from four separate PCR reactions were sequenced directly or were cloned into the plasmid vector pCR 2.1 (Invitrogen, Carlsbad, CA) and then sequenced. The coding sequence of the coat protein gene of RBDV-China was 87.5% (722/825) identical to that isolated from black raspberry (Genbank Accession No. s55890). The predicted amino acid sequences were 91.6% (251/274) identical. Previously, a maximum of five amino acid differences had been observed in the coat proteins of different RBDV strains (1). The 23 differences observed between RBDV-China and the isolate from black raspberry (s55890) confirm that the RBDV in R. multibracteatus is not a greenhouse contaminant but is indeed a unique strain of RBDV. In addition, monoclonal antibodies (MAbs) to RBDV (2) were tested against RBDV-China. In these tests, MAb D1 did not detect RBDV-China, whereas MAb R2 and R5 were able to detect the strain. This is the first strain of RBDV that has been clearly differentiated by MAbs using standard TAS-ELISA tests. Although RBDV is common in commercial Rubus spp. worldwide, to our knowledge, this is the first report of RBDV in R. multibracteatus, and the first report of RBDV from China. The effects of this new strain of RBDV could be more or less severe, or have a different host range than previously studied strains. It is more divergent from the type isolate than any other strain that has been studied to date. Phylogenetic analysis of coat protein genes of RBDV may be useful in understanding the evolution and spread of this virus. References: (1) A. T. Jones et al. Eur. J. Plant Pathol. 106:623, 2000. (2) R. R. Martin. Can. J. Plant. Pathol. 6:264, 1984. (3) A. F. Murant. Raspberry Bushy Dwarf. Page 229 in: Virus Diseases of Small Fruits. R. H. Converse, ed. U.S. Dep. Agric. Agric. Handb. 631, 1987. (4) B. Strik and R. R. Martin. Plant Dis. 87:294, 2003.

Nucleotide Sequence of Rice Dwarf Phytoreovirus Genome Segment 2: Completion of Sequence Analyses of Rice Dwarf Virus

Intervirology ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 6-11 ◽  
Author(s):  
Ichiro Uyeda ◽  
Narushi Suda ◽  
Naoki Yamada ◽  
Hiroshi Kudo ◽  
Kazunori Murao ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Genome Segment ◽  
Dwarf Virus ◽  
Rice Dwarf Virus ◽  
Sequence Analyses
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