Detection of latent Epstein-Barr virus (EBV) DNA in paraffin sections of nasopharyngeal carcinomas expressing no EBV-encoded small RNAs using in situ PCR

1997 ◽  
Vol 142 (9) ◽  
pp. 1743-1756 ◽  
Author(s):  
H. Takeuchi ◽  
R. Kobayashi ◽  
M. Hasegawa ◽  
K. Hirai
Keyword(s):  
Small Rnas ◽  
Epstein Barr Virus ◽  
Paraffin Sections ◽  
In Situ Pcr ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

Epstein–Barr virus plays little role in cervical carcinogenesis in Korean women

2005 ◽  
Vol 15 (2) ◽  
pp. 312-318
Author(s):  
S. S. Seo ◽  
W. H. Kim ◽  
Y. S. Song ◽  
S. H. Kim ◽  
J. W. Kim ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Epstein Barr Virus ◽  
Intraepithelial Neoplasia ◽  
Cervical Carcinogenesis ◽  
Korean Women ◽  
Barr Virus ◽  
Ebv Infection ◽  
Ebv Dna ◽  
Epstein Barr

We examined whether Epstein–Barr virus (EBV) infection plays a role in cervical carcinogenesis in Korean women. EBV infection was examined using polymerase chain reaction (PCR) with two different primer pairs flanking the BamHI “W” fragment of EBV and by EBV-encoded small RNAs (EBER) in situ hybridization in various histologic types of cervical cancer, including 17 cases of squamous cell carcinoma, 36 cases of adenocarcinoma, and 3 cases of small-cell carcinoma. We also evaluated 20 cases of cervical intraepithelial neoplasia and 20 cases of normal uterine cervix. One case of squamous cell carcinoma and three cases of cervical intraepithelial neoplasia were positive for EBV DNA using PCR, but EBER in situ hybridization analysis showed that none of the PCR-positive cases expressed EBER. EBV DNA was not found using PCR in any of the 20 normal uterine cervices. From our results, EBV infection does not seem to play a role in cervical carcinogenesis in Korean women.

Are acetowhite lesions of the cervix correlated to the presence of Epstein-Barr virus DNA?

1997 ◽  
Vol 8 (7) ◽  
pp. 432-436 ◽  
Author(s):  
Ewa Voog ◽  
Anne Ricksten ◽  
Monica Stenglein ◽  
Fredrik Jonassen ◽  
Annika Ternesten ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Predictive Factor ◽  
Epstein Barr Virus ◽  
Control Group ◽  
Study Group ◽  
Hpv Dna ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

The purpose of this study was to investigate whether Epstein-Barr virus (EBV) is associated with acetowhite lesions of the portio cervix, demonstrating koilocytosis and/or cervical intraepithelial neoplasia (CIN) I-III. The study group comprised 37 women admitted to the Department of Gynaecology and Obstetrics, Sahlgrenska University Hospital, Go teborg because of pathological colposcopy or cytology of the portio cervix. Colposcopically, all exhibited acetowhite lesions of the portio cervix. Cells were sampled with a cytobrush for examination for EBV and human papillomavirus (HPV) DNA by polymerase chain reaction (PCR) and a biopsy was taken for histopathology. Biopsies from 5 patients positive for EBV by PCR in cervical cell samples were examined by an in situ hybridization technique for EBER (Epstein-Barr virus encoded RNA), RNAs expressed in latent EBV infection. The control group consisted of women attending the Department of Dermato-Venereology at the same hospital for STD check-up. These had a normal cytology and no signs of acetowhiteness of the portio cervix. In the study group, EBV DNA was found in 30% and HPV DNA in 51%. In the control group 57% exhibited EBV DNA and 23% HPV DNA. EBV was not found to be a predictive factor in the development of koilocytosis and/or CIN I-III. HPV was a predictive factor in acetowhite, koilocytotic lesions. No expression of EBER was found in the 5 biopsies examined by in situ hybridization.

scholarly journals Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR

10.4081/958 ◽  
2009 ◽  
Vol 49 (3) ◽  
pp. 309
Author(s):  
N Marziliano ◽  
E Arbustini ◽  
M Rossi de Gasperis ◽  
S Crovella
Keyword(s):  
Epstein Barr Virus ◽  
In Situ Pcr ◽  
Barr Virus ◽  
Formalin Fixed Paraffin ◽  
Epstein Barr ◽  
Formalin Fixed
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