Tobacco veinal necrosis determinants are unlikely to be located within the 5′ and 3′ terminal sequences of the potato virus Y genome

1997 ◽  
Vol 142 (4) ◽  
pp. 765-779 ◽  
Author(s):  
A. M. Chachulska ◽  
M. Chrzanowska ◽  
C. Robaglia ◽  
W. Zagórski
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Veinal Necrosis

Characterisation of Potato virus Y nnp strain inducing veinal necrosis in pepper: a naturally occurring recombinant strain of PVY

2004 ◽  
Vol 150 (4) ◽  
pp. 709-720 ◽  
Author(s):  
A. Fanigliulo ◽  
S. Comes ◽  
R. Pacella ◽  
B. Harrach ◽  
D. P. Martin ◽  
...  
Keyword(s):  
Potato Virus ◽  
Recombinant Strain ◽  
Potato Virus Y ◽  
Veinal Necrosis ◽  
Naturally Occurring

scholarly journals First Report of the Necrotic Strain of Potato virus Y (PVYN) on Potatoes in the Northwestern United States

Plant Disease ◽  
2002 ◽  
Vol 86 (10) ◽  
pp. 1177-1177 ◽  
Author(s):  
J. M. Crosslin ◽  
P. B. Hamm ◽  
K. C. Eastwell ◽  
R. E. Thornton ◽  
C. R. Brown ◽  
...  
Keyword(s):  
United States ◽  
Monoclonal Antibodies ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Plant Viruses ◽  
Enzyme Analysis ◽  
Rt Pcr ◽  
Veinal Necrosis ◽  
Pcr Products ◽  
Plant Pathol

More than 50 isolates of Potato virus Y (PVY) with characteristics of strains that cause tobacco veinal necrosis (PVYN) were obtained from potatoes (Solanum tuberosum L.) grown in the northwestern United States. These isolates are being characterized at the biological and molecular levels. Isolate RR1 was obtained from leaves of potato cv. Ranger Russet showing distinct mottling and leaf deformity, which is in contrast to the leaf-drop and necrosis usually observed with ordinary strains of PVY (PVYO) in this variety. Isolate AL1 was obtained from tubers of potato cv. Alturas showing distinct internal light brown rings and blotches. When RR1 and AL1 were transmitted to tobacco (Nicotiana tabacum L. cvs. Samsun NN and 423), they caused systemic veinal necrosis, including stem and petiole lesions typical of PVYN strains (2). Symptoms induced by RR1 and AL1 on tobacco appeared 9 to 11 days after inoculation, whereas some other isolates caused delayed veinal necrosis. All isolates that produced veinal necrosis on tobacco were detectable with PVY polyclonal antisera. Potato virus X was not detected by enzyme-linked immunosorbent assay in tobacco plants showing veinal necrosis. Some isolates, including AL1, failed to react in serological tests using PVYN-specific monoclonal antibodies obtained from three commercial sources. Other isolates, including RR1, were detectable with these monoclonal antibodies. Reverse transcription-polymerase chain reaction (RT-PCR) products obtained with primers specific for the coat protein (CP) open reading frame (ORF) were cloned and sequenced. AL1 possesses a CP more closely related to PVYO type isolates, which would account for its failure to react with PVYN monoclonal antibodies. In this regard, AL1 is similar to the PVYN-Wilga isolate (1). Other isolates that are detectable with the PVYN monoclonal antibodies possess a CP more consistent with N strains of the virus. Results of RT-PCR tests using primers derived from the P1 ORF sequence (3), and the restriction enzyme analysis and sequencing of the RT-PCR products, all suggest that AL1 and RR1 are related to European-type members of PVY tuber necrotic (NTN) or N strains. However, other isolates under investigation appear to be more closely related to previously reported North American NTN types (3). The symptomatology of these viruses on tobacco and potato, and the serological and molecular data clearly show that at least two distinct variants of PVYN have been found for the first time in a major potato production area of the United States, and pose a potential threat to the potato industry. References: (1) B. Blanco-Urgoiti et al. Eur. J. Plant Pathol. 104:811, 1998. (2) J. A. de Bokx and H. Huttinga. Potato virus Y. Descriptions of Plant Viruses. No. 242, CMI/AAB, Surrey, England, 1981. (3) R. P. Singh et al. Can J. Plant Pathol. 20:227, 1998.

scholarly journals Identification and Geographic Distribution of Serotypes of Potato Virus Y

Plant Disease ◽  
1997 ◽  
Vol 81 (5) ◽  
pp. 481-484 ◽  
Author(s):  
P. Ellis ◽  
R. Stace-Smith ◽  
G. de Villiers
Keyword(s):  
Monoclonal Antibodies ◽  
North America ◽  
Geographic Distribution ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Seed ◽  
Seed Certification ◽  
Strain Group ◽  
Veinal Necrosis ◽  
Potato Seed Certification

From a panel of 10 monoclonal antibodies (MAbs) prepared against specific isolates representing the three recognized strain groups of potato virus Y (PVY), i.e., common (PVYO), tobacco veinal necrosis (PVYN), and stipple streak (PVYC), seven were selected for serotype analysis. These MAbs were tested for reactivity with 52 PVY strains representing all three strain groups from an international collection. Within the PVYN strain group, five serotypes were identified and designated N1 to N5. The PVYO strain group was more diverse, and nine serotypes were defined and designated O1 to O9. Only one serotype, designated C1, was defined) within the PVYC strain group. The same panel of MAbs was used to test 632 PVY samples collected from potato seed certification plots in North America. Although no PVY(N) serotypes were found, all of the PVYO serotypes were identified, and several samples, tentatively assigned to the C1 serotype, were found.

scholarly journals NtTPN1 : a RPP8 -like R gene required for Potato virus Y -induced veinal necrosis in tobacco

2018 ◽  
Vol 95 (4) ◽  
pp. 700-714 ◽  
Author(s):  
Vincent Michel ◽  
Emilie Julio ◽  
Thierry Candresse ◽  
Julien Cotucheau ◽  
Christophe Decorps ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
R Gene ◽  
Veinal Necrosis

scholarly journals Identification of an Isolate of Potato virus Y-Tuber Necrotic Strain on Clary Sage (Salvia sclarea)

Plant Disease ◽  
2001 ◽  
Vol 85 (11) ◽  
pp. 1207-1207 ◽  
Author(s):  
M. G. Bellardi ◽  
C. Rubies-Autonell ◽  
C. Cerato
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Sandwich Elisa ◽  
Protein A ◽  
Culture Collection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chenopodium Amaranticolor ◽  
Veinal Necrosis ◽  
Salvia Sclarea ◽  
Malformed Leaves

In 1999, clary sage plants (Salvia sclarea L.) at the Herb Garden of Casola Valsenio (Emilia-Romagna Region, northern Italy) exhibited malformed leaves with yellow spots and line patterns. Sap from leaves of symptomatic sage plants caused symptoms in inoculated Chenopodium amaranticolor Coste et Reyn. plants (local chloro-necrotic lesions developed 7 to 10 days after inoculation) and Nicotiana tabacum L. ‘White Burley’ and ‘Samsun’ plants (systemic veinal necrosis developed ≈ 2 weeks after inoculation). Leaves from symptomatic sage plants tested positive for Potato virus Y (PVY) based on immunoelectron microscopy, gold-labeled decoration, and protein A sandwich enzyme-linked immunosorbent assay (ELISA), using antiserum to PVY (PVAS 50a, American Type Culture Collection, Manassas, VA). Double-antibody sandwich-ELISA, using specific monoclonal antibodies (BioReba AG, Reinach, Switzerland) to the tobacco veinal necrosis strain group of PVY (PVY-N), revealed that the PVY isolate from sage belonged to this group. Immunocapture-reverse transcription-polymerase chain reaction, using specific primers for PVY and the tuber necrotic strain of PVY (PVY-NTN), further classified the sage isolate as PVY-NTN (1). PVY-NTN causes serious damage to potato in Europe. Clary sage, one of the most important aromatic plants cultivated worldwide as a source of essential oils, represents a new natural host of PVY-NTN. Reference: (1) H. L. Weidemann and E. Maiss. J. Plant Dis. Prot. 103:337, 1996.

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