Characterization of antibody response to hepatitis C virus protein E2 and significance of hypervariable region 1-specific antibodies in viral neutralization

1997 ◽  
Vol 142 (3) ◽  
pp. 523-534 ◽  
Author(s):  
A. Zibert ◽  
P. Dudziak ◽  
E. Schreier ◽  
M. Roggendorf
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Antibody Response ◽  
Hypervariable Region ◽  
Virus Protein ◽  
Viral Neutralization ◽  
Specific Antibodies ◽  
Hypervariable Region 1

scholarly journals Construction and characterization of chimeric hepatitis C virus E2 glycoproteins: analysis of regions critical for glycoprotein aggregation and CD81 binding

2000 ◽  
Vol 81 (12) ◽  
pp. 2873-2883 ◽  
Author(s):  
Arvind H. Patel ◽  
Jonny Wood ◽  
Francois Penin ◽  
Jean Dubuisson ◽  
J. A. McKeating
Keyword(s):  
Amino Acids ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Protein Misfolding ◽  
Hypervariable Region ◽  
Genotype 1A ◽  
Hypervariable Region 1 ◽  
Glycosylation Sites ◽  
Cd81 Binding

We compared the ability of two closely related truncated E2 glycoproteins (E2660) derived from hepatitis C virus (HCV) genotype 1a strains Glasgow (Gla) and H77c to bind a panel of conformation-dependent monoclonal antibodies (MAbs) and CD81. In contrast to H77c, Gla E2660 formed disulfide-linked high molecular mass aggregates and failed to react with conformation-dependent MAbs and CD81. To delineate amino acid (aa) regions associated with protein aggregation and CD81 binding, several Gla–H77c E2660 chimeric glycoproteins were constructed. Chimeras C1, C2 and C6, carrying aa 525–660 of Gla E2660, produced disulfide-linked aggregates and failed to bind CD81 and conformation-dependent MAbs, suggesting that amino acids within this region are responsible for protein misfolding. The presence of Gla hypervariable region 1 (aa 384–406) on H77 E2660, chimera C4, had no effect on protein folding or CD81 binding. Chimeras C3 and C5, carrying aa 384–524 or 407–524 of Gla E2660, respectively, were recognized by conformation-dependent MAbs and yet failed to bind CD81, suggesting that amino acids in region 407–524 are important in modulating CD81 interaction without affecting antigen folding. Comparison of Gla and H77c E2660 aa sequences with those of genotype 1a and divergent genotypes identified a number of variant amino acids, including two putative N-linked glycosylation sites at positions 476 and 532. However, introduction of G476N–G478S and/or D532N in Gla E2660 had no effect on antigenicity or aggregation.

scholarly journals Virus Isolate-specific Antibodies against Hypervariable Region 1 of the Hepatitis C Virus Second Envelope Protein, gp70

1994 ◽  
Vol 85 (10) ◽  
pp. 987-991 ◽  
Author(s):  
Nobuyuki Kato ◽  
Takahide Nakazawa ◽  
Yuko Ootsuyama ◽  
Kazuo Sugiyama ◽  
Showgo Ohkoshi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Protein ◽  
Virus Isolate ◽  
Hypervariable Region ◽  
Specific Antibodies ◽  
Hypervariable Region 1

scholarly journals Early antibody response against hypervariable region 1 is associated with acute self-limiting infections of hepatitis C virus

Hepatology ◽  
1997 ◽  
Vol 25 (5) ◽  
pp. 1245-1249 ◽  
Author(s):  
A Zibert ◽  
H Meisel ◽  
W Kraas ◽  
A Schulz ◽  
G Jung ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Antibody Response ◽  
Hypervariable Region ◽  
Hypervariable Region 1

scholarly journals Hypervariable region 1 variant acting as TCR antagonist affects hepatitis C virus-specific CD4+T cell repertoire by favoring CD95-mediated apoptosis

2005 ◽  
Vol 78 (2) ◽  
pp. 372-382 ◽  
Author(s):  
Cristiano Scottà ◽  
Loretta Tuosto ◽  
Anna Maria Masci ◽  
Luigi Racioppi ◽  
Enza Piccolella ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
T Cell ◽  
Hypervariable Region ◽  
Cd4 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Hypervariable Region 1
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