The extracellular part of glycoprotein E of bovine herpesvirus 1 is sufficient for complex formation with glycoprotein I but not for cell-to-cell spread

2000 ◽  
Vol 145 (2) ◽  
pp. 333-351 ◽  
Author(s):  
J. Tyborowska ◽  
K. Bieńkowska-Szewczyk ◽  
M. Rychłowski ◽  
J. T. Van Oirschot ◽  
F. A. M. Rijsewijk
Keyword(s):  
Complex Formation ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Glycoprotein I ◽  
Herpesvirus 1 ◽  
Cell Spread

scholarly journals Two Separate Tyrosine-Based YXXL/Φ Motifs within the Glycoprotein E Cytoplasmic Tail of Bovine Herpesvirus 1 Contribute in Virus Anterograde Neuronal Transport

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1025
Author(s):  
Hocine Yezid ◽  
Christian T. Lay ◽  
Katrin Pannhorst ◽  
Shafiqul I. Chowdhury
Keyword(s):  
Epithelial Cells ◽  
Bovine Herpesvirus ◽  
Cytoplasmic Tail ◽  
Anterograde Transport ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Tyrosine Residues ◽  
Neuronal Transport ◽  
Herpesvirus 1 ◽  
Cell Spread

Bovine herpesvirus 1 (BHV-1) causes respiratory infection and abortion in cattle. Following a primary infection, BHV-1 establishes lifelong latency in the trigeminal ganglia (TG). Periodic reactivation of the latent virus in TG neurons results in anterograde virus transport to nerve endings in the nasal mucosa and nasal virus shedding. The BHV-1 glycoprotein E cytoplasmic tail (gE-CT) is necessary for virus cell-to-cell spread in epithelial cells and neuronal anterograde transport. Recently, we identified two tyrosine residues, Y467 and Y563, within the tyrosine-based motifs 467YTSL470 and 563YTVV566, which, together, account for the gE CT-mediated efficient cell-to-cell spread of BHV-1 in epithelial cells. Here, we determined that in primary neuron cultures in vitro, the individual alanine exchange Y467A or Y563A mutants had significantly diminished anterograde axonal spread. Remarkably, the double-alanine-exchanged Y467A/Y563A mutant virus was not transported anterogradely. Following intranasal infection of rabbits, both wild-type (wt) and the Y467A/Y563A mutant viruses established latency in the TG. Upon dexamethasone-induced reactivation, both wt and the mutant viruses reactivated and replicated equally efficiently in the TG. However, upon reactivation, only the wt, not the mutant, was isolated from nasal swabs. Therefore, the gE-CT tyrosine residues Y467 and Y563 together are required for gE CT-mediated anterograde neuronal transport.

Epitopes on glycoprotein E and on the glycoprotein E/glycoprotein I complex of bovine herpesvirus 1 are expressed by all of 222 isolates and 11 vaccine strains

2000 ◽  
Vol 145 (5) ◽  
pp. 921-936 ◽  
Author(s):  
F. A. M. Rijsewijk ◽  
M. J. Kaashoek ◽  
J. P. M. Langeveld ◽  
M. A. Maris-Veldhuis ◽  
J. Magdalena ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Glycoprotein I ◽  
Herpesvirus 1

EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains

2017 ◽  
Vol 28 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Mohini Saini ◽  
B. P. Mishra ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

scholarly journals Safety and immunogenicity of a glycoprotein E gene-deleted bovine herpesvirus 1 strain as a candidate vaccine strain

2016 ◽  
Vol 36 (11) ◽  
pp. 1067-1074
Author(s):  
Marcelo Weiss ◽  
◽  
Deniz Anziliero ◽  
Mathias Martins ◽  
Rudi Weiblen ◽  
...  
Keyword(s):  
Acute Infection ◽  
Clinical Signs ◽  
Bovine Herpesvirus ◽  
Elisa Test ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Virus Shedding ◽  
Group I ◽  
Virus Dose ◽  
Herpesvirus 1

ABSTRACT: A glycoprotein E-deleted Brazilian bovine herpesvirus 1 (BoHV-1gEΔ) was tested regarding to safety and immunogenicity. Intramuscular inoculation of young calves with a high virus dose did not result in clinical signs or virus shedding during acute infection or after dexamethasone administration. Calves vaccinated once IM (group I) or subcutaneously (group II) with live BoHV-1gEΔ or twice with inactivated virus plus aluminum hydroxide (group IV) or Montanide™ (group V) developed VN titers of 2 to 8 (GMT:2); 2 to 4 (GMT:1.65); 2 to 16 (GMT:2.45) and 2 to 128 (GMT:3.9), respectively. All BoHV-1gEΔ vaccinated calves remained negative in an anti-gE ELISA. Lastly, six young calves vaccinated with live BoHV-1gEΔ and subsequently challenged with a virulent BoHV-1 strain shed less virus and developed only mild and transient nasal signs comparing to unvaccinated calves. Thus, the recombinant BoHV-1gEΔ is safe and immunogenic for calves and allows for serological differentiation by a gE-ELISA test.

scholarly journals A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain

2015 ◽  
Vol 48 (9) ◽  
pp. 843-851 ◽  
Author(s):  
M. Weiss ◽  
M.C.S. Brum ◽  
D. Anziliero ◽  
R. Weiblen ◽  
E.F. Flores
Keyword(s):  
Vaccine Strain ◽  
Bovine Herpesvirus ◽  
Candidate Vaccine ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
E Gene ◽  
Herpesvirus 1

Efficacy of a live glycoprotein E-negative bovine herpesvirus 1 vaccine in cattle in the field

Vaccine ◽  
2001 ◽  
Vol 19 (15-16) ◽  
pp. 1924-1930 ◽  
Author(s):  
M.H Mars ◽  
M.C.M de Jong ◽  
P Franken ◽  
J.T van Oirschot
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

A live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine induces a partial cross-protection against caprine herpesvirus 1 infection in goats

2006 ◽  
Vol 113 (3-4) ◽  
pp. 303-308 ◽  
Author(s):  
Julien Thiry ◽  
Maria Tempesta ◽  
Michele Camero ◽  
Elvira Tarsitano ◽  
Anna Lucia Bellacicco ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Cross Protection ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

scholarly journals Intraspecific bovine herpesvirus 1 recombinants carrying glycoprotein E deletion as a vaccine marker are virulent in cattle

2006 ◽  
Vol 87 (8) ◽  
pp. 2149-2154 ◽  
Author(s):  
Benoît Muylkens ◽  
François Meurens ◽  
Frédéric Schynts ◽  
Frédéric Farnir ◽  
Aldo Pourchet ◽  
...  
Keyword(s):  
High Frequency ◽  
Bovine Herpesvirus ◽  
Clinical Score ◽  
Natural Host ◽  
Field Strain ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Control Programmes ◽  
Herpesvirus 1

Vaccines used in control programmes of Bovine herpesvirus 1 (BoHV-1) utilize highly attenuated BoHV-1 strains marked by a deletion of the glycoprotein E (gE) gene. Since BoHV-1 recombinants are obtained at high frequency in experimentally coinfected cattle, the consequences of recombination on the virulence of gE-negative BoHV-1 were investigated. Thus, gE-negative BoHV-1 recombinants were generated in vitro from several virulent BoHV-1 and one mutant BoHV-1 deleted in the gC and gE genes. Four gE-negative recombinants were tested in the natural host. All the recombinants were more virulent than the gE-negative BoHV-1 vaccine and the gC- and gE-negative parental BoHV-1. The gE-negative recombinant isolated from a BoHV-1 field strain induced the highest severe clinical score. Latency and reactivation studies showed that three of the recombinants were reexcreted. Recombination can therefore restore virulence of gE-negative BoHV-1 by introducing the gE deletion into a different virulence background.

Virulence and immunogenicity in calves of thymidine kinase- and glycoprotein E-negative bovine herpesvirus 1 mutants

1996 ◽  
Vol 48 (1-2) ◽  
pp. 143-153 ◽  
Author(s):  
M.J. Kaashoek ◽  
F.A.C. van Engelenburg ◽  
A. Moerman ◽  
A.L.J. Gielkens ◽  
F.A.M. Rijsewijk ◽  
...  
Keyword(s):  
Thymidine Kinase ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1
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