scholarly journals Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus

2000 ◽  
Vol 145 (2) ◽  
pp. 291-300 ◽  
Author(s):  
C. -H. Wang ◽  
Y. -C. Huang
Keyword(s):  
Infectious Bronchitis Virus ◽  
Hypervariable Region ◽  
S1 Gene ◽  
Infectious Bronchitis

scholarly journals Presence of Infectious Bronchitis Virus Strain CK/CH/LDL/97I in the Middle East

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Mustafa Ababneh ◽  
Abd Elhafeed Dalab ◽  
Saad Alsaad ◽  
Mohammad Al-Zghoul
Keyword(s):  
Middle East ◽  
Infectious Bronchitis Virus ◽  
Hypervariable Region ◽  
Disease Outbreaks ◽  
Nucleotide Identity ◽  
Economic Losses ◽  
Strain Identification ◽  
S1 Gene ◽  
Infectious Bronchitis ◽  
First Time

Infectious bronchitis virus (IBV) is a very dynamic and evolving virus, causing major economic losses to the global poultry industry. In early 2011, respiratory disease outbreaks were investigated in Iraq, Jordan, and Saudi Arabia. Five IBV isolates (JOA2, JOA4, Saudi-1, Saudi-2, and Iraqi IBV) were detected by diagnostic-nested nucleocapsid RT-PCR. Strain identification was characterised by sequencing and phylogenetic analysis of the amplified hypervariable region of the spike 1 (S1) gene. These five IBV isolates were found to be of the IBV strain CK/CH/LDL/97I. Nucleotide identity between these five IBV isolates ranged from 96.9% to 99.7%, and between these isolates and the CK/CH/LDL/97I strain in the range of 96.6–99.1%. The sequenced fragment of the S1 gene of the CK/CH/LDL/97I strain had less than 80% nucleotide identity to the IBV vaccine strains commonly used in the Middle East (M41 and H120). The presence of these CK/CH/LDL/97I-like strains may account for vaccination failure against IBV, since all IBV isolates were from vaccinated chickens. In this paper, we documented for the first time the presence of IBV strain CK/CH/LDL/97I in the Middle East. This strain is known to have originated in China and Taiwan.

scholarly journals Typing of Field Isolates of Infectious Bronchitis Virus Based on the Sequence of the Hypervariable Region in the S1 Gene

2003 ◽  
Vol 15 (4) ◽  
pp. 344-348 ◽  
Author(s):  
Chang-Won Lee ◽  
Deborah A. Hilt ◽  
Mark W. Jackwood
Keyword(s):  
Phylogenetic Analysis ◽  
Infectious Bronchitis Virus ◽  
Direct Sequencing ◽  
Hypervariable Region ◽  
The United States ◽  
Rt Pcr ◽  
Universal Primer ◽  
S1 Gene ◽  
Infectious Bronchitis ◽  
Field Isolates

A universal primer set was developed that amplifies a region covering hypervariable region (HVR) 1 and HVR 2 in the S1 gene of the infectious bronchitis virus (IBV). The universality of this primer set was confirmed by testing the reference strains of different serotypes or variants of the IBV present in the United States. An approximately 450-bp region containing HVR 1 and HVR 2 of 7 untyped field isolates obtained in 1999 and 2000 was amplified. Direct sequencing followed by phylogenetic analysis on that region allowed us to type those field isolates that were not typable by reverse transcriptase–polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). Furthermore, it was found that typing by phylogenetic analysis of that region correlates with virus neutralization results. Together with RT-PCR and RFLP, this method will serve as a fast typing method for IBV diagnosis.

scholarly journals Evolutionary implications of genetic variations in the S1 gene of infectious bronchitis virus

1994 ◽  
Vol 34 (3) ◽  
pp. 327-338 ◽  
Author(s):  
Wang Li ◽  
Dave Junker ◽  
Lisa Hock ◽  
Elham Ebiary ◽  
Ellen W. Collisson
Keyword(s):  
Infectious Bronchitis Virus ◽  
Genetic Variations ◽  
S1 Gene ◽  
Infectious Bronchitis

scholarly journals Cocirculation of Four Infectious Bronchitis Virus Lineages in Broiler Chickens in the Eastern Region of Saudi Arabia from 2012 to 2014

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Abdullah I. A. Al-Mubarak ◽  
Anwar A. G. Al-Kubati
Keyword(s):  
Saudi Arabia ◽  
Middle East ◽  
Infectious Bronchitis Virus ◽  
Broiler Chickens ◽  
Direct Sequencing ◽  
Hypervariable Region ◽  
Economic Losses ◽  
Eastern Region ◽  
Infectious Bronchitis ◽  
First Time

Avian infectious bronchitis virus (IBV) is an evolving and dynamic virus that causes major economic losses for the poultry industry worldwide. Continuous evolution and emergence of new variants of this virus are the major challenges for controlling the disease with routine vaccination. Successful vaccination usually requires the use of a homologous vaccine, which in turn necessitates continuous investigation of the circulating strains. Herein, we performed a reverse transcriptase-polymerase chain reaction- (RT-PCR-) based investigation in broiler chicken flocks of the Eastern Region of Saudi Arabia. IBV was detected in 36.5% of the tested flocks (42 out of 115) from January 2012 to March 2014. Direct sequencing of hypervariable region-3 (HVR-3) of the Spike (S)-1 gene was performed, followed by phylogenetic analysis to determine the circulating IBV genotypes. Four lineages appear to coexist in this region, including the GI-13 or 4/91 IBV (31%), GI-16 or CK/CH/LDL/97I IBV (28.6%), GI-1 or Mass IBV (19%), and GI-23 or Middle East IBV (21.4%). The latter lineage include two subgroups: IS/720/99 IBV (16.7%) and IS/Variant2/98 IBV (4.7%). Some of the detections made in the 4/91 and Mass lineages are expected to belong to the vaccine strains. Lineages without a homologous vaccine in use (CK/CH/LDL/97I and Middle East) represent 50% of the isolates recovered in this study. Based on identity with the vaccine sequences, field observations, and frequent detection, these two lineages appear to be out of coverage of the IBV vaccines used in Saudi Arabia. This is the first time to identify Middle East lineage (IS/720/99 IBV and IS/Variant2/98 IBV) in the Eastern Region of Saudi Arabia.

scholarly journals Molecular characterization of isolated infectious bronchitis viruses from affected vaccinated broiler flocks in Syria

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Tamara Al-Jallad ◽  
Morshed Kassouha ◽  
Mohamad Salhab ◽  
Anouar Alomar ◽  
Mouhamad AL-Masalma ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Hypervariable Region ◽  
Effective Vaccine ◽  
Molecular Characteristics ◽  
Poultry Industry ◽  
S1 Gene ◽  
Infectious Bronchitis ◽  
Epidemiological Monitoring ◽  
Immunological Protection

Abstract Background Avian Infectious Bronchitis Virus (IBV) is a highly contagious disease that imposes a huge economic burden on the global poultry industry. IBV contains numerous serotypes and variants with incomplete tenuous cross immunological protection. The failure of currently used vaccines to protect against diverse, circulating IBV strains that are specific to a given region poses a major problem for the poultry industry. Thus, there is an urgent need to conduct studies aimed at genotyping field IB viruses. In this study, we have determined the molecular characteristics of circulating IBV by sequencing the S1 gene of viral isolates from affected previously vaccinated broiler flocks suffering from the disease. Results Ten isolates propagated in embryonated eggs showed an ability to induce typical IBV lesions after three successive viral passages. We performed a nested RT–PCR assay that targeted the hypervariable region 3 (HVR-3) of the S1 gene, and identified the isolates as IBV through sequence analysis. The IBV isolates showed sequence similarity between the Syrian isolates that vary from 96.20 to 100%, and those being closer to the Variant-2 strain IS/1494/06 (EU780077.2) with 97.5 to 99.4% similarities. However, less nucleotide identity was found with sequences belonging to the used vaccine strains such as H120, Mass5, and 4/91. Conclusions This study showed the presence of the Variant-2 strain circulating in Syrian broiler flocks showing signs of IBV disease. Currently, there is no commercial effective vaccine which protects birds against the Variant-2 strain. Continuous monitoring procedures should be taken to control and limit the spread of the IBV Variant-2 strain. This research emphasizes both the importance of epidemiological monitoring in intensive poultry farming for novel pathogens and the use of local isolates as future vaccine targets.

scholarly journals Phylogeny and S1 Gene Variation of Infectious Bronchitis Virus Detected in Broilers and Layers in Turkey

2016 ◽  
Vol 60 (3) ◽  
pp. 596-602 ◽  
Author(s):  
Huseyin Yilmaz ◽  
Eda Altan ◽  
Utku Y. Cizmecigil ◽  
Aydin Gurel ◽  
Gulay Yuzbasioglu Ozturk ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
S1 Gene ◽  
Gene Variation ◽  
Infectious Bronchitis

A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus

Vaccine ◽  
2003 ◽  
Vol 21 (21-22) ◽  
pp. 2730-2736 ◽  
Author(s):  
Michael A. Johnson ◽  
Catherine Pooley ◽  
Jagoda Ignjatovic ◽  
Scott G. Tyack
Keyword(s):  
Infectious Bronchitis Virus ◽  
S1 Gene ◽  
Infectious Bronchitis ◽  
Fowl Adenovirus
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