The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus)

2015 ◽  
Vol 160 (10) ◽  
pp. 2651-2654 ◽  
Author(s):  
Li Su ◽  
Zhengnan Li ◽  
Mike Bernardy ◽  
Paul A. Wiersma ◽  
Zhihui Cheng ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Genome Organization ◽  
Complete Nucleotide Sequence ◽  
Streak Virus

Complete nucleotide sequence of Northern cereal mosaic virus and its genome organization

2000 ◽  
Vol 145 (7) ◽  
pp. 1373-1384 ◽  
Author(s):  
F. Tanno ◽  
A. Nakatsu ◽  
S. Toriyama ◽  
M. Kojima
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Genome Organization ◽  
Complete Nucleotide Sequence

scholarly journals Complete Nucleotide Sequence and Genome Organization of Hibiscus Chlorotic Ringspot Virus, a New Member of the Genus Carmovirus: Evidence for the Presence and Expression of Two Novel Open Reading Frames

2000 ◽  
Vol 74 (7) ◽  
pp. 3149-3155 ◽  
Author(s):  
Mei Huang ◽  
Dora Chin-Yen Koh ◽  
Li-Juan Weng ◽  
Min-Li Chang ◽  
Yun-Kiam Yap ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Genome Organization ◽  
Complete Nucleotide Sequence ◽  
Full Length ◽  
Open Reading Frames ◽  
Ringspot Virus ◽  
In Vitro Translation ◽  
Cdna Clones ◽  
High Amino Acid ◽  
Reading Frames

ABSTRACT The complete nucleotide sequence of hibiscus chlorotic ringspot virus (HCRSV) was determined. The genomic RNA (gRNA) is 3,911 nucleotides long and has the potential to encode seven viral proteins in the order of 28 (p28), 23 (p23), 81 (p81), 8 (p8), 9 (p9), 38 (p38), and 25 (p25) kDa. Excluding two unique open reading frames (ORFs) encoding p23 and p25, the ORFs encode proteins with high amino acid similarity to those of carmoviruses. In addition to gRNA, two 3′-coterminated subgenomic RNA (sgRNA) species were identified. Full-length cDNA clones derived from gRNA and sgRNA were constructed under the control of a T7 promoter. Both capped and uncapped transcripts derived from the full-length genomic cDNA clone were infectious. In vitro translation and mutagenesis assays confirmed that all the predicted ORFs except the ORF encoding p8 are translatable, and the two novel ORFs (those encoding p23 and p25) may be functionally indispensable for the viral infection cycle. Based on virion morphology and genome organization, we propose that HCRSV be classified as a new member of the genus Carmovirus in familyTombusviridae.

scholarly journals Complete Genome Sequence of the Largest Known Flavi-Like Virus, Diaphorina citri flavi-like virus , a Novel Virus of the Asian Citrus Psyllid, Diaphorina citri

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Emilyn E. Matsumura ◽  
Luca Nerva ◽  
Jared C. Nigg ◽  
Bryce W. Falk ◽  
Shahideh Nouri
Keyword(s):  
Nucleotide Sequence ◽  
Small Rna ◽  
Complete Genome Sequence ◽  
Genome Organization ◽  
Complete Nucleotide Sequence ◽  
Transcriptome Sequencing ◽  
Asian Citrus Psyllid ◽  
Diaphorina Citri ◽  
Rt Pcr ◽  
Novel Virus

A novel flavi-like virus tentatively named Diaphorina citri flavi-like virus (DcFLV) was identified in field populations of Diaphorina citri through small RNA and transcriptome sequencing followed by reverse transcription (RT)-PCR. We report here the complete nucleotide sequence and genome organization of DcFLV, the largest flavi-like virus identified to date.

The complete nucleotide sequence and genome organization of lychnis mottle virus

2015 ◽  
Vol 160 (11) ◽  
pp. 2891-2894 ◽  
Author(s):  
Ran Hee Yoo ◽  
Fumei Zhao ◽  
Seungmo Lim ◽  
Davaajargal Igori ◽  
Su-Heon Lee ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Genome Organization ◽  
Complete Nucleotide Sequence ◽  
Mottle Virus
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