Development of a nested PCR and its internal control for the detection of African swine fever virus (ASFV) in Ornithodoros erraticus

2005 ◽  
Vol 151 (4) ◽  
pp. 819-826 ◽  
Author(s):  
A. P. Basto ◽  
R. S. Portugal ◽  
R. J. Nix ◽  
C. Cartaxeiro ◽  
F. Boinas ◽  
...  
Keyword(s):  
Internal Control ◽  
Nested Pcr ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

Development of a real‐time PCR assay for detection of African swine fever virus with an endogenous internal control

2020 ◽  
Vol 67 (6) ◽  
pp. 2446-2454 ◽  
Author(s):  
Yin Wang ◽  
Lizhe Xu ◽  
Lance Noll ◽  
Colin Stoy ◽  
Elizabeth Porter ◽  
...  
Keyword(s):  
Real Time ◽  
Internal Control ◽  
Real Time Pcr ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Pcr Assay

Development of an internal control for the detection of the African swine fever virus by PCR

2002 ◽  
Vol 16 (3) ◽  
pp. 237-242 ◽  
Author(s):  
M Gonzague ◽  
C Plin ◽  
L Bakkali-Kassimi ◽  
A Boutrouille ◽  
C Cruciere
Keyword(s):  
Internal Control ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

scholarly journals Molecular monitoring of African swine fever virus using surveys targeted at adult Ornithodoros ticks : a re-evaluation of Mkuze Game Reserve, South Africa

2009 ◽  
Vol 76 (4) ◽  
Author(s):  
L.F. Arnot ◽  
J.T. Du Toit ◽  
A.D.S. Bastos
Keyword(s):  
South Africa ◽  
Internal Control ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Dna Integrity ◽  
Molecular Monitoring ◽  
Sylvatic Cycle ◽  
Pcr Screening ◽  
Game Reserve

The Mkuze Game Reserve (MGR), in north-eastern KwaZulu-Natal Province, South Africa is an African swine fever virus (ASF) controlled area. In a survey conducted in 1978, ASF prevalence in warthogs and Ornithodoros ticks in MGR was determined to be 2 % and 0.06 %, respectively. These values, acknowledged as being unusually low compared to other East and southern African ASF-positive sylvatic-cycle host populations, have not been assessed since. The availability of a sensitive PCR-based virus detection method, developed specifically for the sylvatic tampan host, prompted a re-evaluation of ASF virus (ASFV) prevalence in MGR ticks. Of the 98 warthog burrows inspected for Ornithodoros presence, 59 (60.2 %) were found to contain tampans and tick sampling was significantly male-biased. Whilst gender sampling-bias is not unusual, the 27 % increase in infestation rate of warthog burrows since the 1978 survey is noteworthy as it anticipates a concomitant increase in ASFV prevalence, particularly in light of the high proportion (75 %) of adult ticks sampled. However, despite DNA integrity being confirmed by internal control amplification of the host 16S gene, PCR screening failed to detect ASFV. These results suggest that ASFV has either disappeared from MGR or if present, is localized, occurring at exceptionally low levels. Further extensive surveys are required to establish the ASFV status of sylvatic hosts in this controlled area.

Genetic markers of the African swine fever virus

2020 ◽  
Vol 23 (04) ◽  
pp. 21-26
Author(s):  
A.K. Sibgatullova ◽  
◽  
M.E. Vlasov ◽  
I.A. Titov ◽  
◽  
...  
Keyword(s):  
Genetic Markers ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

Multigene families in African swine fever virus: family 110.

1990 ◽  
Vol 64 (5) ◽  
pp. 2064-2072 ◽  
Author(s):  
J M Almendral ◽  
F Almazán ◽  
R Blasco ◽  
E Viñuela
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Multigene Families ◽  
Virus Family

GS-441524 inhibits African swine fever virus infection in vitro

2021 ◽  
pp. 105081
Author(s):  
Zhao Huang ◽  
Lang Gong ◽  
Zezhong Zheng ◽  
Qi Gao ◽  
Xiongnan Chen ◽  
...  
Keyword(s):  
Virus Infection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus
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