Comparative sequence analysis of the South African vaccine strain and two virulent field isolates of Lumpy skin disease virus

2003 ◽  
Vol 148 (7) ◽  
pp. 1335-1356 ◽  
Author(s):  
P. D. Kara ◽  
C. L. Afonso ◽  
D. B. Wallace ◽  
G. F. Kutish ◽  
C. Abolnik ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
South African ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Skin Disease ◽  
Comparative Sequence Analysis ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Field Isolates ◽  
Comparative Sequence

Experimental lumpy skin disease virus infection of cattle: comparison of a field strain and a vaccine strain

2019 ◽  
Vol 164 (12) ◽  
pp. 2931-2941 ◽  
Author(s):  
Janika Möller ◽  
Tom Moritz ◽  
Kore Schlottau ◽  
Kiril Krstevski ◽  
Donata Hoffmann ◽  
...  
Keyword(s):  
Virus Infection ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Skin Disease ◽  
Field Strain ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

Immunogenicity of a recombinant lumpy skin disease virus (neethling vaccine strain) expressing the rabies virus glycoprotein in cattle

Vaccine ◽  
2002 ◽  
Vol 20 (21-22) ◽  
pp. 2693-2701 ◽  
Author(s):  
Kate Aspden ◽  
Alberdina A van Dijk ◽  
John Bingham ◽  
Dermot Cox ◽  
Jo-Ann Passmore ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rabies Virus ◽  
Vaccine Strain ◽  
Skin Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Rabies Virus Glycoprotein ◽  
Virus Glycoprotein

scholarly journals Genomic Sequence of the New Attenuated Vaccine Strain Neethling-RIBSP of the Lumpy Skin Disease Virus

2020 ◽  
Vol 9 (26) ◽  
Author(s):  
M. B. Orynbayev ◽  
R. K. Nissanova ◽  
T. U. Argimbayeva ◽  
K. D. Zakarya ◽  
B. S. Myrzakhmetova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Genome Sequencing ◽  
Vaccine Strain ◽  
Skin Disease ◽  
Genomic Sequence ◽  
Draft Genome ◽  
Differentiation Markers ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

ABSTRACT We report here the draft genome sequence of the new attenuated strain Neethling-RIBSP of the lumpy skin disease virus, obtained by sequential and alternating passages in cell culture and developing chicken embryos. Genome sequencing allowed the identification of differentiation markers of the new strain.

scholarly journals An investigation of possible routes of transmission of lumpy skin disease virus (Neethling)

1995 ◽  
Vol 114 (1) ◽  
pp. 219-226 ◽  
Author(s):  
V. M. Carn ◽  
R. P. Kitching
Keyword(s):  
South African ◽  
Disease Virus ◽  
Skin Disease ◽  
Neutralizing Antibodies ◽  
Clinical Signs ◽  
Intravenous Route ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Clinical Scoring ◽  
Intravenous Inoculation

SUMMARYBritish cattle were infected with the South African (Neethling) strain of lumpy skin disease virus (LSDV) and their clinical signs monitored over a 3-week period. Different routes of infection were assessed for effect on the clinical characteristics of the disease by using a clinical scoring system. Neither of 2 animals inoculated onto the conjunctival sac showed clinical signs or seroconverted. The intradermal route produced local lesions in 21 of 25 animals, and generalized infection in 4. In contrast the intravenous route produced generalized lesions in 8 of 11 animals. Seven uninfected animals were housed in contact with infected animals for 1 month. None developed clinical signs or produced detectable serum neutralizing antibodies. Six of seven of these animals were then challenged and were fully susceptible to infection. The results suggest that the transmission of LSDV between animals by contagion is extremely inefficient, and that parenteral inoculation of virus is required to establish infection. The high proportion of animals with generalized disease following intravenous inoculation implies that naturally occurring cases of generalized LSD may follow spread by intravenously feeding arthropods.

REAL TIME PCR FOR THE DETECTION OF FIELD ISOLATES OF LUMPY SKIN DISEASE VIRUS IN CLINICAL SAMPLES FROM CAT-TLE

2018 ◽  
Vol 53 (2) ◽  
pp. 422-429 ◽  
Author(s):  
Ya.E. Pestova ◽  
E.E. Artyukhova ◽  
E.E. Kostrova ◽  
I.N. Shumoliva ◽  
A.V. Kononov ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Skin Disease ◽  
Clinical Samples ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Field Isolates

scholarly journals Comparative Evaluation of Lumpy Skin Disease Virus-Based Live Attenuated Vaccines

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 473
Author(s):  
Andy Haegeman ◽  
Ilse De Leeuw ◽  
Laurent Mostin ◽  
Willem Van Campe ◽  
Laetitia Aerts ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Vaccination Campaign ◽  
Ease Of Use ◽  
Vaccine Failure ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
The Balkans ◽  
The Past ◽  
Preventative Strategy

Vaccines form the cornerstone of any control, eradication and preventative strategy and this is no different for lumpy skin disease. However, the usefulness of a vaccine is determined by a multiplicity of factors which include stability, efficiency, safety and ease of use, to name a few. Although the vaccination campaign in the Balkans against lumpy skin disease virus (LSDV) was successful and has been implemented with success in the past in other countries, data of vaccine failure have also been reported. It was therefore the purpose of this study to compare five homologous live attenuated LSDV vaccines (LSDV LAV) in a standardized setting. All five LSDV LAVs studied were able to protect against a challenge with virulent LSDV. Aside from small differences in serological responses, important differences were seen in side effects such as a local reaction and a Neethling response upon vaccination between the analyzed vaccines. These observations can have important implications in the applicability in the field for some of these LSDV LAVs.

scholarly journals Production of small ruminant morbillivirus, rift valley fever virus and lumpy skin disease virus in CelCradle™ -500A bioreactors

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Halima Rhazi ◽  
Najete Safini ◽  
Karima Mikou ◽  
Meryeme Alhyane ◽  
Khalid Omari Tadlaoui ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Cell Factories

Abstract Background Animal vaccination is an important way to stop the spread of diseases causing immense damage to livestock and economic losses and the potential transmission to humans. Therefore effective method for vaccine production using simple and inexpensive bioprocessing solutions is very essential. Conventional culture systems currently in use, tend to be uneconomic in terms of labor and time involved. Besides, they offer a limited surface area for growth of cells. In this study, the CelCradle™-500A was evaluated as an alternative to replace conventional culture systems in use such as Cell factories for the production of viral vaccines against small ruminant morbillivirus (PPR), rift valley fever virus (RVF) and lumpy skin disease virus (LSD). Results Two types of cells Vero and primary Lamb Testis cells were used to produce these viruses. The study was done in 2 phases as a) optimization of cell growth and b) virus cultivation. Vero cells could be grown to significantly higher cell densities of 3.04 × 109 using the CelCradle™-500A with a shorter doubling time as compared to 9.45 × 108 cells in Cell factories. This represents a 19 fold increase in cell numbers as compared to seeding vs only 3.7 fold in Cell factories. LT cells achieved modestly higher cell densities of 6.7 × 108 as compared to 6.3 × 108 in Cell factories. The fold change in densities for these cells was 3 fold in the CelCradle™-500A vs 2.5 fold in Cell factories. The titers in the conventional system and the bioreactor were not significantly different. However, the Cell-specific virus yield for rift valley fever virus and lumpy skin disease virus are higher (25 virions/cell for rift valley fever virus, and 21.9 virions/cell for lumpy skin disease virus versus 19.9 virions/cell for rift valley fever virus and 10 virions/cell for lumpy skin disease virus). Conclusions This work represents a novel study for primary lamb testis cell culture in CellCradle™-500A bioreactors. In addition, on account of the high cell densities obtained and the linear scalability the titers could be further optimized using other culture process such us perfusion.

Molecular characterization of lumpy skin disease virus in Iran (2014–2018)

2021 ◽  
Author(s):  
Zeinab Hedayati ◽  
Hamid Reza Varshovi ◽  
Ali Mohammadi ◽  
Mohammad Tabatabaei
Keyword(s):  
Molecular Characterization ◽  
Disease Virus ◽  
Skin Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus
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