Preparation of a Conjugate of Mitomycin C and Anti-Neural Cell Adhesion Molecule Monoclonal Antibody for Specific Chemotherapy Against Biliary Tract Carcinoma

Surgery Today ◽  
1998 ◽  
Vol 28 (11) ◽  
pp. 1217-1220 ◽  
Author(s):  
Jun-ichi Tanaka ◽  
Etsuko Sato ◽  
Yuri Saito ◽  
Tomoyuki Kusano ◽  
Kenji Koyama
Keyword(s):  
Monoclonal Antibody ◽  
Cell Adhesion ◽  
Mitomycin C ◽  
Biliary Tract ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Biliary Tract Carcinoma ◽  
Neural Cell Adhesion

scholarly journals Mapping of three carbohydrate attachment sites in embryonic and adult forms of the neural cell adhesion molecule.

1984 ◽  
Vol 99 (5) ◽  
pp. 1848-1855 ◽  
Author(s):  
K L Crossin ◽  
G M Edelman ◽  
B A Cunningham
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Cell Adhesion ◽  
Sialic Acid ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Attachment Sites ◽  
Neural Cell Adhesion

The sialic-rich carbohydrate moiety of the neural cell adhesion molecule (N-CAM) undergoes major structural changes during development and plays a significant role in altering the homophilic binding of the molecule. In order to understand the mechanism of these changes, a cyanogen bromide (CNBr) fragment that contained 90% of the sialic acid of N-CAM was isolated and characterized according to the number of carbohydrate attachment sites and reactivity with specific monoclonal antibodies. The CNBr sialopeptide migrated on SDS PAGE as a broad zone of Mr 42,000-60,000. Upon treatment with neuraminidase, it was converted to a single component of Mr 42,000, and subsequent, limited treatment with endoglycosidase F gave four evenly spaced components of Mr 35,000-42,000, suggesting that it contained three attachment sites for N-linked oligosaccharides. The fragment reacted with monoclonal antibody 15G8, which detects the sialic acid in embryonic N-CAM, and with a monoclonal antibody, anti-(N-CAM) No. 2. Treatment with neuraminidase or with endoglycosidase F destroyed reactivity with 15G8 but not with anti-(N-CAM) No. 2. A similar CNBr sialopeptide was obtained from adult N-CAM; it contained sialic acid, had three N-linked oligosaccharides and reacted with anti-(N-CAM) No. 2 but not with 15G8 monoclonal antibodies. A peptide fragment, Fr2, comprising the NH2 terminal and middle regions of the molecule yielded a CNBr fragment closely similar to the fragment obtained from the whole molecule. The CNBr fragment from Fr2 reacted with monoclonal antibody anti-(N-CAM) No. 2. Fr1, comprising the NH2 terminal region alone, failed to react. These data confirm that the majority of the sialic acid is localized in the middle region of the N-CAM molecule and support the hypothesis that embryonic to adult conversion of N-CAM is the result of differences in sialidase or sialytransferase activity.

JLP5B9: new monoclonal antibody against polysialylated neural cell adhesion molecule is of value in phenotyping lung cancer

2000 ◽  
Vol 233 (1-2) ◽  
pp. 21-31 ◽  
Author(s):  
Maguy Del Rio ◽  
Pascal Demoly ◽  
Aurelia M.C Koros ◽  
Jean-Claude Laurent ◽  
Jean-Claude Mani ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Monoclonal Antibody ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Neural Cell Adhesion

scholarly journals Generation of Monoclonal Antibody against Canine Neural-Cell Adhesion Molecule

2008 ◽  
Vol 70 (8) ◽  
pp. 845-847
Author(s):  
Yosuke UEMATSU ◽  
Tomohiro YAMAGUCHI ◽  
Ayumi KOIKE ◽  
Hiroko YAGIHARA ◽  
Daisuke HASEGAWA ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Neural Cell Adhesion

Monoclonal antibody UJ13A recognizes the neural cell adhesion molecule (NCAM)

1989 ◽  
Vol 44 (6) ◽  
pp. 1062-1068 ◽  
Author(s):  
K. Patel ◽  
R. J. Rossell ◽  
S. Bourne ◽  
S. E. Moore ◽  
F. S. Walsh ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Neural Cell Adhesion
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