Levels of carbonyl groups in plasma proteins of type 2 diabetes mellitus subjects

1999 ◽  
Vol 36 (4) ◽  
pp. 179-183 ◽  
Author(s):  
P. Odetti ◽  
S. Garibaldi ◽  
G. Noberasco ◽  
I. Aragno ◽  
S. Valentini ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Plasma Proteins ◽  
Carbonyl Groups

scholarly journals Multiple Glycation Sites in Blood Plasma Proteins as an Integrated Biomarker of Type 2 Diabetes Mellitus

2019 ◽  
Vol 20 (9) ◽  
pp. 2329 ◽  
Author(s):  
Alena Soboleva ◽  
Gregory Mavropulo-Stolyarenko ◽  
Tatiana Karonova ◽  
Domenika Thieme ◽  
Wolfgang Hoehenwarter ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Blood Plasma ◽  
Plasma Proteins ◽  
Linear Discriminant ◽  
Slow Development ◽  
Specificity And Sensitivity ◽  
Blood Plasma Proteins

Type 2 diabetes mellitus (T2DM) is one of the most widely spread metabolic diseases. Because of its asymptomatic onset and slow development, early diagnosis and adequate glycaemic control are the prerequisites for successful T2DM therapy. In this context, individual amino acid residues might be sensitive indicators of alterations in blood glycation levels. Moreover, due to a large variation in the half-life times of plasma proteins, a generalized biomarker, based on multiple glycation sites, might provide comprehensive control of the glycemic status across any desired time span. Therefore, here, we address the patterns of glycation sites in highly-abundant blood plasma proteins of T2DM patients and corresponding age- and gender-matched controls by comprehensive liquid chromatography-mass spectrometry (LC-MS). The analysis revealed 42 lysyl residues, significantly upregulated under hyperglycemic conditions. Thereby, for 32 glycation sites, biomarker behavior was demonstrated here for the first time. The differentially glycated lysines represented nine plasma proteins with half-lives from 2 to 21 days, giving access to an integrated biomarker based on multiple protein-specific Amadori peptides. The validation of this biomarker relied on linear discriminant analysis (LDA) with random sub-sampling of the training set and leave-one-out cross-validation (LOOCV), which resulted in an accuracy, specificity, and sensitivity of 92%, 100%, and 85%, respectively.

Plasma proteome changes in subjects with Type 2 diabetes mellitus with a low or high early insulin response

2008 ◽  
Vol 114 (7) ◽  
pp. 499-507 ◽  
Author(s):  
Tea Sundsten ◽  
Björn Zethelius ◽  
Christian Berne ◽  
Peter Bergsten
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Plasma Protein ◽  
Plasma Proteins ◽  
Insulin Response ◽  
Β Cell ◽  
Protein Levels ◽  
Early Insulin Response

Circulating proteins contribute to the pathogenesis of T2DM (Type 2 diabetes mellitus) in various ways. The aim of the present study was to investigate variations in plasma protein levels in subjects with T2DM and differences in β-cell function, characterized by the EIR (early insulin response), and to compare these protein levels with those observed in individuals with NGT (normal glucose tolerance). Ten subjects with NGT+high EIR, ten with T2DM+high EIR, and ten with T2DM+low EIR were selected from the community-based ULSAM (Uppsala Longitudinal Study of Adult Men) cohort. Plasma protein profiling was performed using SELDI-TOF (surface-enhanced laser-desorption ionization–time-of-flight) MS. In total, nine plasma proteins differed between the three study groups (P<0.05, as determined by ANOVA). The levels of two forms of transthyretin, haemoglobin α-chain and haemoglobin β-chain were decreased in plasma from subjects with T2DM compared with subjects with NGT, irrespective of the EIR of the subjects. Apolipoprotein H was decreased in plasma from individuals with T2DM+high EIR compared with subjects with NGT. Four additional unidentified plasma proteins also varied in different ways between the experimental groups. In conclusion, the proteins detected in the present study may be related to the development of β-cell dysfunction.

Carbonylated Plasma Proteins As Potential Biomarkers of Obesity Induced Type 2 Diabetes Mellitus

2014 ◽  
Vol 13 (11) ◽  
pp. 5081-5093 ◽  
Author(s):  
Ravi Chand Bollineni ◽  
Maria Fedorova ◽  
Matthias Blüher ◽  
Ralf Hoffmann
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Plasma Proteins ◽  
Potential Biomarkers

Abstract #1214: Decreasing Hypoglycemia Without Compromising Efficacy in an Aging Type 2 Diabetes Mellitus (T2Dm) Population

2015 ◽  
Vol 21 ◽  
pp. 280-281
Author(s):  
Medha Munshi ◽  
Jasvinder Gill ◽  
Jason Chao ◽  
Elena Nikonova ◽  
Andreas Stuhr ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus

Abstract #517: Predicting Risk of Osteoporotic Fracture by Bone Ultrasonography in Type 2 Diabetes Mellitus

2015 ◽  
Vol 21 ◽  
pp. 106
Author(s):  
Franco Grimaldi ◽  
Laura Tonutti ◽  
Claudia Cipri ◽  
Cecilia Motta ◽  
Maria Antonietta Pellegrini ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Osteoporotic Fracture ◽  
Bone Ultrasonography
Sign in / Sign up

Export Citation Format

Share Document