Is anti-pyruvate dehydrogenase complex a predictor of the development of primary biliary cirrhosis?

2001 ◽  
Vol 36 (4) ◽  
pp. 284-285
Author(s):  
Gotaro Toda
Keyword(s):  
Primary Biliary Cirrhosis ◽  
Pyruvate Dehydrogenase ◽  
Pyruvate Dehydrogenase Complex ◽  
Biliary Cirrhosis ◽  
Dehydrogenase Complex

Human Pyruvate Dehydrogenase Complex as An Autoantigen in Primary Biliary Cirrhosis

1993 ◽  
Vol 85 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Jeremy M. Palmer ◽  
Margaret F. Bassendine ◽  
Oliver F. W. James ◽  
Stephen J. Yeaman
Keyword(s):  
Primary Biliary Cirrhosis ◽  
Pyruvate Dehydrogenase ◽  
Pyruvate Dehydrogenase Complex ◽  
Heart Tissue ◽  
Immune Reactivity ◽  
Biliary Cirrhosis ◽  
Antimitochondrial Antibodies ◽  
Future Work ◽  
Dehydrogenase Complex

1. The sera of more than 90% of patients with primary biliary cirrhosis contain antimitochondrial antibodies which react with the E2 component of the pyruvate dehydrogenase complex, identified as the major autoantigen in primary biliary cirrhosis. All previous studies in this area have utilized protein derived from animal tissue or have used recombinant human pyruvate dehydrogenase complex E2 expressed in Escherichia coli. 2. We report the preparation and characterization of native pyruvate dehydrogenase complex and pyruvate dehydrogenase complex E2 from human heart tissue and its application in studies of immune reactivity with the sera of patients with primary biliary cirrhosis. 3. The immune reactivity of sera from patients with primary biliary cirrhosis versus the bovine and human E2/X components of pyruvate dehydrogenase complex was indistinguishable in both immunoblotting and the more sensitive e.l.i.s.a. 4. These findings suggest that the reactivity of sera from patients with primary biliary cirrhosis against the major autoantigen of the disease is a property of that antigen, independent of its human or bovine origin. Furthermore, this justifies the use of bovine pyruvate dehydrogenase complex in past and future work on primary biliary cirrhosis antibody reactivity.

The E1 α and β Subunits of the Pyruvate Dehydrogenase Complex are M2‘d’ and M2‘e’ Autoantigens in Primary Biliary Cirrhosis

1989 ◽  
Vol 77 (4) ◽  
pp. 365-368 ◽  
Author(s):  
Shelley P. M. Fussey ◽  
Margaret F. Bassendine ◽  
Dorothy Fittes ◽  
Ian B. Turner ◽  
Oliver F. W. James ◽  
...  
Keyword(s):  
Liver Disease ◽  
Primary Biliary Cirrhosis ◽  
Chronic Liver Disease ◽  
Pyruvate Dehydrogenase ◽  
Positive Response ◽  
Pyruvate Dehydrogenase Complex ◽  
Biliary Cirrhosis ◽  
Normal Women ◽  
Histological Stage ◽  
Dehydrogenase Complex

1. Sera from 76 patients with primary biliary cirrhosis (PBC) and 66 control subjects (53 with chronic liver disease and 13 healthy normal women) were immunoblotted against purified E1 component of bovine pyruvate dehydrogenase complex (PDC) and bacterial PDC. 2. Thirty-one out of seventy-six (41%) sera from PBC patients showed a positive response to bovine E1α, and five of these 31 (7% of total) reacted with bovine E1β. None of the control sera reacted with bovine E1 α or β. 3. None of the PBC sera that recognized bovine E1 subunits reacted with bacterial PDC E1. 4. In the PBC patients there was no correlation between presence of antibodies to E1α and β subunits and histological stage of the disease. 5. Our data demonstrate that the E1α and β components of mammalian PDC are the M2‘d’ and ‘e’ mitochondrial autoantigens, respectively.

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