Induction of hepatic stellate cell proliferation by LPS-stimulated peripheral blood mononuclear cells from patients with liver cirrhosis

2000 ◽  
Vol 35 (3) ◽  
pp. 214-220 ◽  
Author(s):  
Kyoko Toda ◽  
Naoki Kumagai ◽  
Kanji Tsuchimoto ◽  
Hiroyuki Inagaki ◽  
Tatsuo Suzuki ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Liver Cirrhosis ◽  
Peripheral Blood Mononuclear Cells ◽  
Hepatic Stellate Cell ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Stellate Cell ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Extracts from Microalga Chlorella sorokiniana Exert an Anti-Proliferative Effect and Modulate Cytokines in Sheep Peripheral Blood Mononuclear Cells

Animals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 45 ◽  
Author(s):  
Maria Ciliberti ◽  
Marzia Albenzio ◽  
Matteo Francavilla ◽  
Gianluca Neglia ◽  
Luigi Esposito ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Animal Feed ◽  
Lipid Fraction ◽  
Chlorella Sorokiniana ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Unsaponified Fraction

The objective of this experiment was to study the effects of the unsaponified fraction (UP), the acetylated unsaponified fraction (AUP), and the total lipid fraction (TL) extracted and purified from Chlorella sorokiniana (CS) on the proliferation and cytokine profile of sheep peripheral blood mononuclear cells (PBMCs). Cells were cultured with 0.4 mg/mL and 0.8 mg/mL concentrations of each extract (UP, AUP, and TL fractions) and activated with 5 μg/mL concanavalin A (ConA) and 1 μg/mL lipopolysaccharide (LPS) at 37 °C for 24 h. PBMCs cultured with ConA and LPS represented the stimulated cells (SC), and PBMCs without ConA and LPS represented the unstimulated cells (USC). Cell-free supernatants were collected to determine IL-10, IL-1β, and IL-6 secretions; on cells, measurement of proliferation was performed. All the extracts tested significantly decreased the cell proliferation; in particular, the UP fraction at 0.4 mg/mL showed the lowest proliferative response. Furthermore, at 0.8 mg/mL, the UP fraction enhanced IL-10 secretion. On the contrary, the TL fraction at 0.4 mg/mL induced an increase in IL-10, IL-6, and, to a lesser extent, IL-1β secretions by cells. The AUP fraction did not change cytokine secretion. The results demonstrated that CS extracts could be useful ingredients in animal feed in order to minimize the use of antibiotics by modulating cell proliferation and cytokine response.

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