Prolyl 4-hydroxylase inhibitor (HOE 077) prevents TIMP-1 gene expression in rat liver fibrosis

1999 ◽  
Vol 34 (3) ◽  
pp. 376-377 ◽  
Author(s):  
Isao Sakaida ◽  
Koichi Uchida ◽  
Koji Hironaka ◽  
Kiwamu Okita
Keyword(s):  
Gene Expression ◽  
Liver Fibrosis ◽  
Rat Liver ◽  
Rat Liver Fibrosis

scholarly journals Treatment Efficiency of Different Routes of Bone Marrow-Derived Mesenchymal Stem Cell Injection in Rat Liver Fibrosis Model

2018 ◽  
Vol 48 (5) ◽  
pp. 2161-2171 ◽  
Author(s):  
Naglaa K. Idriss ◽  
Hayam G. Sayyed ◽  
Amany Osama ◽  
Dina Sabry
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Stem Cell ◽  
Growth Factor ◽  
Liver Fibrosis ◽  
Mesenchymal Stem Cell ◽  
Rat Liver ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rat Liver Fibrosis ◽  
Recovery Group

Background/Aims: The most appropriate route for bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in the management of liver fibrosis remains controversial. This study investigated the therapeutic efficacy of intravenous and intrasplenic BM-MSC transplantation on carbon tetrachloride (CCl4)-induced rat liver fibrosis. Methods: Fifty rats were divided into 5 groups (n = 10 rats per group): healthy control group, CCl4 group, CCl4/ recovery group, CCl4/BM-MSC intravenous group, and CCl4/BM-MSC intrasplenic group. BM-MSCs were isolated, labeled with green fluorescent protein (GFP), and injected into fibrotic rats either intravenously or intrasplenically. Gene expression of interleukins (IL-1β and IL-6), interferon (INF)-γ, hepatic growth factor, and the hepatocyte-specific marker cytokeratin 18 was estimated by quantitative real-time reverse transcription-polymerase chain reaction. Vascular endothelial growth factor and connective tissue growth factor was detected by western blot analysis and enzyme-linked immunosorbent assay, respectively. At 2 weeks after intravenous and intrasplenic BM-MSC injections, GFP-positive cells were detected in liver tissue. Results: Both routes achieved a similar enhancement of liver function, which was confirmed by histopathological examination. The intravenous route was more effective than the intrasplenic route in reducing gene expression levels of IL-1β, IL-6, and INF-γ. However, fibrotic changes were still observed in the recovery group. Conclusion: Intravenous BM-MSC injection was an efficient and appropriate route for BM-MSC transplantation for the management of liver fibrosis.

Gene expression of interstitial collagenase in both progressive and recovery phase of rat liver fibrosis induced by carbon tetrachloride

2000 ◽  
Vol 33 (2) ◽  
pp. 224-235 ◽  
Author(s):  
Tetsu Watanabe ◽  
Maki Niioka ◽  
Shigenari Hozawa ◽  
Kaori Kameyama ◽  
Tatsuhiko Hayashi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Carbon Tetrachloride ◽  
Liver Fibrosis ◽  
Rat Liver ◽  
Recovery Phase ◽  
Rat Liver Fibrosis ◽  
Interstitial Collagenase

scholarly journals Actions of Huangqi decoction against rat liver fibrosis: a gene expression profiling analysis

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Gui-biao Zhang ◽  
Ya-nan Song ◽  
Qi-long Chen ◽  
Shu Dong ◽  
Yi-yu Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Liver Fibrosis ◽  
Gene Expression Profiling ◽  
Rat Liver ◽  
Expression Profiling ◽  
Rat Liver Fibrosis ◽  
Profiling Analysis
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