Absence of Mycobacterium paratuberculosis DNA in intestinal tissues from Crohn's disease by nested polymerase chain reaction

1999 ◽  
Vol 34 (2) ◽  
pp. 200-206 ◽  
Author(s):  
Kosuke Kanazawa ◽  
Yoichi Haga ◽  
Osamu Funakoshi ◽  
Hitoshi Nakajima ◽  
Akihiro Munakata ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Mycobacterium Paratuberculosis ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Search for Mycobacterium paratuberculosis DNA in orofacial granulomatosis and oral Crohn’s disease tissue by polymerase chain reaction

Gut ◽  
1997 ◽  
Vol 41 (5) ◽  
pp. 646-650 ◽  
Author(s):  
M P Riggio ◽  
J Gibson ◽  
A Lennon ◽  
D Wray ◽  
D G MacDonald
Keyword(s):  
Polymerase Chain Reaction ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Oral Lesions ◽  
Mycobacterium Paratuberculosis ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Orofacial Granulomatosis ◽  
Polymerase Chain ◽  
Normal Controls

Background—Although intestinal Crohn’s disease has long been suspected to have a mycobacterial cause, possible mycobacterial involvement in orofacial granulomatosis (OFG) and oral lesions of Crohn’s disease has not yet been investigated.Aims—As the slow growingMycobacterium paratuberculosis has been implicated in the aetiology of intestinal Crohn’s disease, the potential involvement of this mycobacterial species in OFG and oral lesions of Crohn’s disease was investigated.Patients—To attempt detection of the organism in OFG and oral Crohn’s disease tissue samples, a polymerase chain reaction (PCR) assay was used on archival formalin fixed, paraffin wax embedded oral tissue sections from 30 patients with OFG, seven with Crohn’s disease, and 12 normal controls.Methods—The PCR assay used was based on primers targeting the 5′ region of the multicopy IS900 DNA insertion element of the M paratuberculosis genome. In order to achieve maximum sensitivity, two rounds of PCR were carried out and amplicons confirmed by Southern blot hybridisation to a digoxigenin labelled IS900 DNA probe.Results—None of the OFG and oral lesions of Crohn’s disease samples were positive forMparatuberculosis and all normal controls were also negative.Conclusions—These results suggest that M paratuberculosis may not be a major aetiological agent in OFG or oral Crohn’s disease lesions, although the use of paraffin wax embedded tissue as opposed to fresh tissue as a sample source could underestimate the true prevalence of the organism.

Detection of Mycobacterium paratuberculosis by Polymerase Chain Reaction in Children with Crohn's Disease

1994 ◽  
Vol 169 (2) ◽  
pp. 449-451 ◽  
Author(s):  
B. Dell'Isola ◽  
C. Poyart ◽  
O. Goulet ◽  
J. F. Mougenot ◽  
E. S. Journo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Mycobacterium Paratuberculosis ◽  
Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document