Tubular toxicity of cyclosporine A and the influence of endothelin-1 in renal cell culture models (LLC-PK1 and MDCK)

1997 ◽  
Vol 11 (6) ◽  
pp. 778-783 ◽  
Author(s):  
Lothar Bernd Zimmerhackl ◽  
Hector Mesa ◽  
Frank Krämer ◽  
Christina Kölmel ◽  
Gerd Wiegele ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cyclosporine A ◽  
Renal Cell ◽  
Endothelin 1 ◽  
Renal Cell Culture ◽  
Culture Models ◽  
Tubular Toxicity ◽  
Cell Culture Models

Renal cell culture models: Contribution to the understanding of nephrotoxic mechanisms

2008 ◽  
pp. 223-249 ◽  
Author(s):  
Paul Jennings ◽  
Christian KoppelstÄtter ◽  
Judith Lechner ◽  
Walter Pfaller
Keyword(s):  
Cell Culture ◽  
Renal Cell ◽  
Renal Cell Culture ◽  
Culture Models ◽  
Cell Culture Models

The anti-epileptic drug substance vigabatrin inhibits taurine transport in intestinal and renal cell culture models

2014 ◽  
Vol 473 (1-2) ◽  
pp. 395-397 ◽  
Author(s):  
Jakob Plum ◽  
Martha Kampp Nøhr ◽  
Steen H. Hansen ◽  
René Holm ◽  
Carsten Uhd Nielsen
Keyword(s):  
Cell Culture ◽  
Renal Cell ◽  
Drug Substance ◽  
Taurine Transport ◽  
Anti Epileptic Drug ◽  
Renal Cell Culture ◽  
Culture Models ◽  
Cell Culture Models

Renal cell culture models: Contribution to the understanding of nephrotoxic mechanisms

2003 ◽  
pp. 115-147 ◽  
Author(s):  
Paul Jennings ◽  
Christian Koppelstätter ◽  
Mark J. Helbert ◽  
Walter Pfaller
Keyword(s):  
Cell Culture ◽  
Renal Cell ◽  
Renal Cell Culture ◽  
Culture Models ◽  
Cell Culture Models

Morphological analysis of renal cell culture models of calcium phosphate stone formation

1997 ◽  
Vol 25 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Y. Naito ◽  
Y. Ohtawara ◽  
S. Kageyama ◽  
M. Nakano ◽  
A. Ichiyama ◽  
...  
Keyword(s):  
Cell Culture ◽  
Calcium Phosphate ◽  
Renal Cell ◽  
Morphological Analysis ◽  
Stone Formation ◽  
Renal Cell Culture ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

scholarly journals Turnover of the Active Fraction of IRS1 Involves Raptor-mTOR- and S6K1-Dependent Serine Phosphorylation in Cell Culture Models of Tuberous Sclerosis

2006 ◽  
Vol 26 (17) ◽  
pp. 6425-6434 ◽  
Author(s):  
O. Jameel Shah ◽  
Tony Hunter
Keyword(s):  
Cell Culture ◽  
Tuberous Sclerosis ◽  
High Speed ◽  
Serine Phosphorylation ◽  
Feedback Signal ◽  
Insulin Receptor Substrates ◽  
Igf I ◽  
Culture Models ◽  
Cell Culture Models

ABSTRACT The TSC1-TSC2/Rheb/Raptor-mTOR/S6K1 cell growth cassette has recently been shown to regulate cell autonomous insulin and insulin-like growth factor I (IGF-I) sensitivity by transducing a negative feedback signal that targets insulin receptor substrates 1 and 2 (IRS1 and -2). Using two cell culture models of the familial hamartoma syndrome, tuberous sclerosis, we show here that Raptor-mTOR and S6K1 are required for phosphorylation of IRS1 at a subset of serine residues frequently associated with insulin resistance, including S307, S312, S527, S616, and S636 (of human IRS1). Using loss- and gain-of-function S6K1 constructs, we demonstrate a requirement for the catalytic activity of S6K1 in both direct and indirect regulation of IRS1 serine phosphorylation. S6K1 phosphorylates IRS1 in vitro on multiple residues showing strong preference for RXRXXS/T over S/T,P sites. IRS1 is preferentially depleted from the high-speed pellet fraction in TSC1/2-deficient mouse embryo fibroblasts or in HEK293/293T cells overexpressing Rheb. These studies suggest that, through serine phosphorylation, Raptor-mTOR and S6K1 cell autonomously promote the depletion of IRS1 from specific intracellular pools in pathological states of insulin and IGF-I resistance and thus potentially in lesions associated with tuberous sclerosis.

Cell culture models of the ocular barriers

2005 ◽  
Vol 60 (2) ◽  
pp. 207-225 ◽  
Author(s):  
Margit Hornof ◽  
Elisa Toropainen ◽  
Arto Urtti
Keyword(s):  
Cell Culture ◽  
Culture Models ◽  
Cell Culture Models
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