Liquid-liquid extraction of a Schistosoma mansoni recombinant protein from an Escherichia coli extract in aqueous two-phase system

2000 ◽  
Vol 23 (5) ◽  
pp. 435-438 ◽  
Author(s):  
A. C. Chaves ◽  
N. Lucena-Silva ◽  
F. G. C. Abath ◽  
V. R. A. Pereira ◽  
J. L. Lima-Filho ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Schistosoma Mansoni ◽  
Liquid Extraction ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction

scholarly journals Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqueous two-phase system

2014 ◽  
Vol 375 ◽  
pp. 104-109 ◽  
Author(s):  
M.N. Carneiro-da-Cunha ◽  
K.P.S. Souza ◽  
A.M.O. Mota ◽  
J.A. Teixeira ◽  
C.S. Porto ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Liquid Extraction ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction

scholarly journals Study of caffeine and coumarin extraction kinetics in an aqueous two-phase system based on polyethylene glycol 1500

2022 ◽  
Vol 1212 (1) ◽  
pp. 012020
Author(s):  
I V Zinov’eva
Keyword(s):  
Biological Activity ◽  
Liquid Extraction ◽  
Phase System ◽  
Plant Origin ◽  
Extraction Kinetics ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Food Industries ◽  
Liquid Liquid Extraction ◽  
Polyethylene Glycol 1500

Abstract Caffeine and coumarin are organic compounds of plant origin, which have biological activity and have found wide application in medicine, pharmaceutical, perfumery and food industries. Recovery of caffeine and coumarin from aqueous solutions using liquid-liquid extraction is the most effective method. In the present work the kinetic dependences of caffeine and coumarin in the aqueous two-phase system (ATPS) of PEG 1500 - Na2SO4 - H2O were experimentally obtained. It was established that equilibrium in the system is achieved after 17 minutes for caffeine and after 12 minutes for coumarin. The effect of ultrasound on the extraction rate of caffeine and coumarin in an aqueous two-phase system was also studied.

scholarly journals Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

2003 ◽  
Vol 46 (4) ◽  
pp. 741-750 ◽  
Author(s):  
Xu Yan ◽  
Maria Aparecida Souza ◽  
Marcela Z. R. Pontes ◽  
Michele Vitolo ◽  
Adalberto Pessoa Júnior
Keyword(s):  
Reactive Dyes ◽  
Liquid Extraction ◽  
Phase System ◽  
Two Phase ◽  
Affinity Ligands ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction ◽  
Commercial Scale ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.

scholarly journals Copper recovery from ore by liquid–liquid extraction using aqueous two-phase system

2012 ◽  
Vol 237-238 ◽  
pp. 209-214 ◽  
Author(s):  
Leandro Rodrigues de Lemos ◽  
Igor José Boggione Santos ◽  
Guilherme Dias Rodrigues ◽  
Luis Henrique Mendes da Silva ◽  
Maria C. Hespanhol da Silva
Keyword(s):  
Liquid Extraction ◽  
Copper Recovery ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction

Liquid–liquid extraction of proteases from fermented broth by PEG/citrate aqueous two-phase system

2008 ◽  
Vol 47 (4) ◽  
pp. 716-721 ◽  
Author(s):  
T.S. Porto ◽  
G.M. Medeiros e Silva ◽  
C.S. Porto ◽  
M.T.H. Cavalcanti ◽  
B.B. Neto ◽  
...  
Keyword(s):  
Liquid Extraction ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction ◽  
Fermented Broth

An aqueous two-phase system to pre-purify a heterologously produced siderophore

TECHNOLOGY ◽  
2016 ◽  
Vol 04 (03) ◽  
pp. 135-138
Author(s):  
Mahmoud Kamal Ahmadi ◽  
Samar Fawaz ◽  
Blaine A. Pfeifer
Keyword(s):  
Escherichia Coli ◽  
Natural Products ◽  
Cell Growth ◽  
Complex Nature ◽  
Phase System ◽  
Heterologous Production ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Purification System ◽  
Surrogate Host

Natural products span broad activities and applications; however, their access and production are often limited by native cellular sources. As a result, the heterologous production of a siderophore termed yersiniabactin (Ybt) was completed using the surrogate host Escherichia coli. Post-production and purification steps are complicated by the complex nature of most media used for cell growth, prompting the development in this work of an aqueous two-phase pre-purification system capable of rapidly and simply enhancing the concentration of the target Ybt compound.

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