Rapid titer assay of adenovirus containing green fluorescent protein gene using flow cytometric analysis

2000 ◽  
Vol 22 (5) ◽  
pp. 403-406 ◽  
Author(s):  
M. T. Park ◽  
G. M. Lee
Keyword(s):  
Green Fluorescent Protein ◽  
Protein Gene ◽  
Fluorescent Protein ◽  
Flow Cytometric Analysis ◽  
Green Fluorescent Protein Gene ◽  
Flow Cytometric ◽  
Green Fluorescent

Efficient Knockout of Transplanted Green Fluorescent Protein Gene in Medaka Using TALENs

2014 ◽  
Vol 16 (6) ◽  
pp. 674-683 ◽  
Author(s):  
Chao Qiu ◽  
Bin Cheng ◽  
Yunsheng Zhang ◽  
Rong Huang ◽  
Lanjie Liao ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Protein Gene ◽  
Fluorescent Protein ◽  
Green Fluorescent Protein Gene ◽  
Green Fluorescent

Stress-survival responses of a carbon-starvedp-nitrophenol-mineralizingMoraxellastrain in river water

2005 ◽  
Vol 51 (3) ◽  
pp. 223-229 ◽  
Author(s):  
Michael Moore ◽  
Jack Trevors ◽  
Hung Lee ◽  
Kam Tin Leung
Keyword(s):  
Green Fluorescent Protein ◽  
River Water ◽  
Water Samples ◽  
Protein Gene ◽  
Fluorescent Protein ◽  
Carbon Starvation ◽  
Green Fluorescent Protein Gene ◽  
River Water Samples ◽  
Stress Survival ◽  
Green Fluorescent

The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1–2 d. In the buffer system, the 1- and 2-day carbon-starved Moraxella cultures survived about 150-, 200-, and 100-fold better than the non-starved cultures when exposed to 43.5 °C, 2.7 mol/L NaCl, and 500 µmol/L H2O2for 4 h, respectively. A green fluorescent protein gene- (gfp) labelled derivative of the Moraxella strain was used to examine the stress-resistant responses of the bacterium in natural river water microcosms. The carbon-starved gfp-labelled Moraxella strain also showed stress-resistant responses against heat, osmotic, and oxidative stresses in the river water samples. Despite the stress-tolerant capability of the carbon-starved gfp-labelled Moraxella cells, they did not exhibit any survival advantage over their non-starved counterparts when inoculated into river water microcosms and incubated at 10 and 22 °C for 14 d.Key words: carbon starvation, stress-survival responses, Moraxella, p-nitrophenol, green fluorescent protein gene.

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