Sesame (Sesamum indicum L.) is an annual plant belonging to the Pedaliaceae family which is the oldest of the oilseed plants. Sesame is known as the king of oilseeds because its seeds contain high oil content (50-60%). The objective of the study was to evaluate the genetic variation of sesame accessions based on ten SSR markers. The results showed that all sesame accessions showed high genetic similarity among individuals in each accession. Polymorphism information content ranged from 0.24 (TNB17) to 0.37 (MT20). HO varied from 0.04 (MT30) to 0.25 (GENE1). The highest HE was 0.37 (MT20) and the lowest HE was 0.28 (TNB17). The results also displayed the high genetic diversity among 7 sesame accessions. The genetic diversity distance varied from 0.0 to 1.0. Dendrogram analysis divided 7 sesame accessions into 5 clear groups at an average genetic distance of 0.25. The results achieved would be useful information for genetic evaluation and sesame breeding development in the future.