Localization of endothelin A receptors in the rat pituitary TSH cells: light- and electron-microscopic immunohistochemical studies

2000 ◽  
Vol 302 (1) ◽  
pp. 85-94 ◽  
Author(s):  
Sonoko Furuya ◽  
Takeshi Hiroe ◽  
Tuyoshi Ozaki ◽  
Misato Takimoto ◽  
Seiji Hori
Keyword(s):  
Electron Microscopic ◽  
Rat Pituitary ◽  
Tsh Cells ◽  
Endothelin A ◽  
Immunohistochemical Studies

Ultrastructural Immunocytochemistry of Five Rat Pituitary Adenomas

1980 ◽  
Vol 38 ◽  
pp. 724-725
Author(s):  
D. J. McComb ◽  
N. Ryan ◽  
E. Horvath ◽  
K. Kovacs ◽  
E. Nagy ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Pituitary Tumors ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Transmission Electron ◽  
Rat Pituitary ◽  
Radiation Induced ◽  
Group 5 ◽  
Group 2 ◽  
Microscopic Techniques

Conventional light and electron microscopic techniques failed to clarify the cellular composition and derivation of spontaneous and induced, intrasellar and transplanted pituitary adenomas in rats (1). In the present work, electron microscopic immunocytochemistry was applied to evaluate five adenohypo-physial tumors using a technique described by Moriarty and Garner (2). Spontaneously occurring pituitary adenomas (group 1) were harvested from aging female Long-Evans rats. R-Amsterdam rats were treated with 2 x 1.0 mg estrone acetate (HogivaI) s.c. weekly for 6 months. Pituitary adenomas in excess of 30 mg were removed from these animals to make up the tumors of group 2. Groups 3 and 4 consisted of estrogen-induced autonomous transplan¬ted pituitary tumors MtT.WlO and MtT.F4. Group 5 was a radiation-induced transplanted autonomous pituitary tumor MtT.W5. The tumors of groups 3,4 and 5 were allowed to proliferate in host rats 6-8 weeks prior to removal for processing. Tissue was processed for transmission electron microscopy (glutaraldehyde fixation, OsO4 postfixation and epoxy resin embedding), and electron microscopic immunocytochemistry (3% paraformaldehyde fixation and Araldite embedding).

TWO CASES OF ACQUIRED TOXOPLASMIC LYMPHADENITIS. Light and Electron Microscopic and Immunohistochemical Studies

1988 ◽  
Vol 38 (12) ◽  
pp. 1565-1573
Author(s):  
Masafumi Ito ◽  
Kazuo Hara ◽  
Shinsuke Saga ◽  
Junpei Asai ◽  
Soichi Iijima
Keyword(s):  
Electron Microscopic ◽  
Immunohistochemical Studies

scholarly journals Subcellular distribution of secretogranins I and II in GH3 rat tumoral prolactin (PRL) cells as revealed by electron microscopic immunocytochemistry.

1989 ◽  
Vol 37 (9) ◽  
pp. 1329-1336 ◽  
Author(s):  
C Tougard ◽  
L E Nasciutti ◽  
R Picart ◽  
A Tixier-Vidal ◽  
W B Huttner
Keyword(s):  
Electron Microscope ◽  
Secretory Granules ◽  
Hormone Treatment ◽  
Pituitary Cell ◽  
Gh3 Cells ◽  
Cell Models ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Normal Cells ◽  
Rat Pituitary

The GH3 rat pituitary cell line which secretes prolactin (PRL) is characterized by the paucity and small size of secretory granules. We looked for the presence, in these cells and in normal PRL cells, of two acidic tyrosine-sulfated proteins which are widely distributed in dense-core secretory granules of endocrine and neuronal cells, secretogranins I and II, using immunofluorescence and electron microscope immunoperoxidase techniques. Both secretogranins were detected in secretory granules of GH3 cells and of normal cells. Moreover, with our pre-embedding approach, secretogranins were localized within some RER cisternae and within all sacules of the Golgi stacks in both PRL cell models. A few small vesicles, large dilated vacuolar or multivesicular structures, and some lysosome-like structures were also immunoreactive. Double localization of secretogranins and PRL performed on GH3 cells by immunofluorescence indicated that all cells contained secretogranins I and II, whereas only 50-70% of the cells contained PRL. Moreover, in the case of hormone treatment known to increase the number of secretory granules, most if not all mature secretory granules were immunoreactive for secretogranins, whereas in certain cells some of the granules were apparently not immunoreactive for PRL. These immunocytochemical observations show that GH3 cells, which under normal conditions form only a small number of secretory granules, produce secretogranins and package them into these granules.

Inflammatory pseudotumor of the stomach: Report of a highly infiltrative case with electron microscopic and immunohistochemical studies

2008 ◽  
Vol 43 (1-2) ◽  
pp. 65-70 ◽  
Author(s):  
Kenichi Kojimahara ◽  
Makio Mukai ◽  
Kazuto Yamazaki ◽  
Taketo Yamada ◽  
Tatsuro Katayama ◽  
...  
Keyword(s):  
Inflammatory Pseudotumor ◽  
Electron Microscopic ◽  
Immunohistochemical Studies

scholarly journals Characteristics of compensatory hypertrophied muscle in the rat: I. Electron microscopic and immunohistochemical studies

1996 ◽  
Vol 246 (3) ◽  
pp. 325-334 ◽  
Author(s):  
Tetsuro Tamaki ◽  
Akira Akatsuka ◽  
Masayoshi Tokunaga ◽  
Shuichi Uchiyama ◽  
Takemasa Shiraishi
Keyword(s):  
Electron Microscopic ◽  
Immunohistochemical Studies
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