Characterization of cultured human prostatic epithelial cells by cluster designation antigen expression

2001 ◽  
Vol 305 (3) ◽  
pp. 389-397 ◽  
Author(s):  
Alvin Liu ◽  
Donna Peehl
Keyword(s):  
Epithelial Cells ◽  
Antigen Expression ◽  
Cluster Designation

scholarly journals Shiga Toxin (Stx)-Binding Glycosphingolipids of Primary Human Renal Cortical Epithelial Cells (pHRCEpiCs) and Stx-Mediated Cytotoxicity

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 139
Author(s):  
Johanna Detzner ◽  
Elisabeth Krojnewski ◽  
Gottfried Pohlentz ◽  
Daniel Steil ◽  
Hans-Ulrich Humpf ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Shiga Toxin ◽  
Saturated Fatty Acids ◽  
Human Kidney ◽  
Enterohemorrhagic Escherichia Coli ◽  
Highly Sensitive ◽  
Liquid Ordered ◽  
Uremic Syndrome

Human kidney epithelial cells are supposed to be directly involved in the pathogenesis of the hemolytic–uremic syndrome (HUS) caused by Shiga toxin (Stx)-producing enterohemorrhagic Escherichia coli (EHEC). The characterization of the major and minor Stx-binding glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer), respectively, of primary human renal cortical epithelial cells (pHRCEpiCs) revealed GSLs with Cer (d18:1, C16:0), Cer (d18:1, C22:0), and Cer (d18:1, C24:1/C24:0) as the dominant lipoforms. Using detergent-resistant membranes (DRMs) and non-DRMs, Gb3Cer and Gb4Cer prevailed in the DRM fractions, suggesting their association with microdomains in the liquid-ordered membrane phase. A preference of Gb3Cer and Gb4Cer endowed with C24:0 fatty acid accompanied by minor monounsaturated C24:1-harboring counterparts was observed in DRMs, whereas the C24:1 fatty acid increased in relation to the saturated equivalents in non-DRMs. A shift of the dominant phospholipid phosphatidylcholine with saturated fatty acids in the DRM to unsaturated species in the non-DRM fractions correlated with the GSL distribution. Cytotoxicity assays gave a moderate susceptibility of pHRCEpiCs to the Stx1a and Stx2a subtypes when compared to highly sensitive Vero-B4 cells. The results indicate that presence of Stx-binding GSLs per se and preferred occurrence in microdomains do not necessarily lead to a high cellular susceptibility towards Stx.

Tracheobronchial epithelium of the sheep: III. Carbohydrate histochemical and cytochemical characterization of secretory epithelial cells

1988 ◽  
Vol 221 (1) ◽  
pp. 540-549 ◽  
Author(s):  
Andrew T. Mariassy ◽  
Judith A. St. George ◽  
Susan J. Nishio ◽  
Charles G. Plopper
Keyword(s):  
Epithelial Cells ◽  
Tracheobronchial Epithelium

Characterization of liver epithelial cells transfected withmyc and/orras oncogenes

1991 ◽  
Vol 36 (5) ◽  
pp. 642-652 ◽  
Author(s):  
Stephen C. Strom ◽  
John B. Faust ◽  
Erika Cappelluti ◽  
Robert B. Harris ◽  
Narendra D. Lalwani
Keyword(s):  
Epithelial Cells ◽  
Liver Epithelial Cells

Characterization of cadmium-induced apoptosis in rat lung epithelial cells: evidence for the participation of oxidant stress

Toxicology ◽  
1999 ◽  
Vol 133 (1) ◽  
pp. 43-58 ◽  
Author(s):  
B.A. Hart ◽  
C.H. Lee ◽  
G.S. Shukla ◽  
A. Shukla ◽  
M. Osier ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oxidant Stress ◽  
Lung Epithelial Cells ◽  
Rat Lung ◽  
Lung Epithelial ◽  
Induced Apoptosis
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