Secretory cell types and cell proliferation of human bronchial epithelial cells in an organ-culture system

1998 ◽  
Vol 293 (3) ◽  
pp. 573-577 ◽  
Author(s):  
R. Bals ◽  
F. Gamarra ◽  
A. Kaps ◽  
S. Grundler ◽  
R. M. Huber ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Organ Culture ◽  
Culture System ◽  
Secretory Cell ◽  
Cell Types ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Organ Culture System

The Role of Oxidative Stress in Apoptosis and Cell Proliferation of Human Bronchial Epithelial Cells

2021 ◽  
Vol 55 (3) ◽  
pp. 283-289
Author(s):  
Hasret Ecevit ◽  
Meral Urhan-Kucuk ◽  
Haluk Uluca ◽  
Duygu Tap ◽  
Abdullah Arpaci
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

Stimulatory effects of hepatocyte growth factor on normal and neoplastic human bronchial epithelial cells

1995 ◽  
Vol 268 (6) ◽  
pp. L1012-L1020 ◽  
Author(s):  
P. Singh-Kaw ◽  
R. Zarnegar ◽  
J. M. Siegfried
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Western Blotting ◽  
Cell Types ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Brdu Labeling ◽  
Hepatocyte Growth

We examined the mitogenic, chemoinvasive, and chemotactic effects of hepatocyte growth factor (HGF) toward normal and neoplastic human epithelial cells derived from the bronchial mucosa. Primary cultures of human bronchial epithelial cells (HBE cells), immortalized bronchial epithelial cells (IB3-1 cells), and cells derived from a squamous cell carcinoma of the lung (128-88T cells) were used as targets. HGF was mitogenic for all three cell types as measured by bromodeoxyuridine (BrdU) labeling and colony-forming efficiency (CFE). With the use of BrdU labeling, 9.8-16.8% of nuclei were labeled in controls vs. 56.9-65.6% labeled nuclei in cells treated with HGF. HGF stimulated colony formation 3.6-6.2-fold over untreated control. Analysis by reverse transcription-polymerase chain reaction demonstrated the presence of the c-met gene, the receptor for HGF, in all three cell types. Cell lysates from all three cell types contained proteins that were recognized by a c-met antibody as determined by Western blotting. The gene for HGF was not expressed in any of the cell types, although it was expressed in control MRC5 fibroblasts. No HGF protein could be detected by Western blotting in the conditioned medium from epithelial cells, although it was readily detectable in medium conditioned by lung fibroblasts. HGF proved to be a powerful chemotactic agent for all three cell types and also stimulated invasion into Matrigel, an artificial basement membrane. The results indicate HGF acts mainly as a paracrine growth factor for cells derived from the human bronchus, and may play a role in the growth and progression of lung tumors.

scholarly journals STING Mediates Cytosolic Dna-Induced Muc5ac Mrna Expression in Human Bronchial Epithelial Cells

2020 ◽  
Author(s):  
Masaya Ohta ◽  
Yutaka Nishida ◽  
Hisako Yagi ◽  
Aikira Aizawa ◽  
Takahito Oyanagi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Cell Types ◽  
Bronchial Epithelial Cells ◽  
Epithelial Cell Line ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Dna Sensing ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

Abstract Background: Non-autologous and autologous cytosolic DNA are recognized as danger signals by cytoplasmic sensor molecules that activate signal-transduction pathways. An important molecule in cytosolic DNA sensing is stimulator of interferon genes (STING), an endoplasmic reticulum protein activated by cyclic GMP–AMP (cGAMP) produced in response to cytosolic DNA. STING is important for innate immune responses to cytosolic DNA in immune cells; however, knowledge about its role in bronchial epithelial cells is limited. Methods: We stimulated NCI-H292 cells with poly(dA:dT) and silenced STING and other regulatory proteins, and then determined MUC5AC mRNA expression levels. Results: Cytosolic DNA increased the expression of a major respiratory mucin protein, MUC5AC, in the human respiratory epithelial cell line NCI-H292 in a STING-dependent manner. Introducing poly(dA:dT) into the cytoplasm induced MUC5AC and interferon-β (IFNβ) expression. Silencing STING by RNA interference decreased poly(dA:dT)-induced MUC5AC mRNA expression but increased IFN-β mRNA levels. Furthermore, cGAMP treatment increased MUC5AC expression but not IFN-β expression. In contrast, silencing retinoic acid-inducible gene-I (RIG-I), which is a component of a different nucleic acid-sensing system, suppressed poly(dA:dT)-induced IFN-β expression and increased MUC5AC expression. Conclusions: Unlike its role in other cell types, in human bronchial epithelial cells, STING is central to cytosolic DNA-induced MUC5AC expression, whereas IFN-β expression is dependent on RIG-I. Our data indicate a functional interaction between the STING and RIG-I pathways, suggesting the existence of intricate and cell-specific cytosolic DNA-sensing systems.

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