Expression of cystatin-related protein and of the C3-component of prostatic-binding protein during postnatal development in the rat ventral prostate and lacrimal gland

1998 ◽  
Vol 292 (1) ◽  
pp. 115-128 ◽  
Author(s):  
Inge Vercaeren ◽  
Hilde Vanaken ◽  
J. Van Dorpe ◽  
Guido Verhoeven ◽  
W. Heyns
Keyword(s):  
Postnatal Development ◽  
Lacrimal Gland ◽  
Binding Protein ◽  
Ventral Prostate ◽  
Related Protein ◽  
Rat Ventral Prostate

scholarly journals Androgen-sensitive spermine-binding protein of rat ventral prostate. Purification of the protein and characterization of the hormonal effect

1979 ◽  
Vol 184 (2) ◽  
pp. 431-440 ◽  
Author(s):  
G Mezzetti ◽  
R Loor ◽  
S Liao
Keyword(s):  
Binding Protein ◽  
Sodium Dodecyl ◽  
Deae Cellulose ◽  
Binding Activity ◽  
Ventral Prostate ◽  
Hormonal Effect ◽  
Rat Ventral Prostate ◽  
Acidic Fraction ◽  
Androgen Effect

The rat ventral prostate contains a cytosol protein that can non-covalently bind spermine much more tightly than spermidine or other natural diamines. The protein has been purified to homogeneity, as judged by electrophoresis in urea- and sodium dodecyl sulphate-containing polyacrylamide gels. The protein, with or without spermine bound to it, sediments at 3 S in a sucrose gradient with or without 0.4 M-KCl. The molecular weight of the protein is about 30 000. Each molecule of the binding protein can bind one molecule of spermine. In the prostate of rats injected with cycloheximide, the protein appears to have a half-life of about 3.5 h. The spermine-binding activity of an acidic fraction obtained by DEAE-cellulose chromatography of the prostate cytosol proteins is reduced by about 40–60% within 20–40 h after castration. This effect is reversed very rapidly within 15–30 min by intraperitoneal injection of 5 alpha-dihydrotestosterone. The hormonal effect is androgen-specific and is not mimicked by dexamethasone or oestradiol-17 beta. The androgen effect was reduced significantly when rats were injected with cycloheximide or actinomycin D, suggesting that the acidic protein may be one of the earliest proteins induced by androgen in the rat ventral prostate.

Postnatal development of lectin binding sites in the rat ventral prostate

1989 ◽  
Vol 21 (11) ◽  
pp. 651-658 ◽  
Author(s):  
Ch. Hauke ◽  
R. Horn ◽  
W. Breuer ◽  
F. Sinowatz
Keyword(s):  
Postnatal Development ◽  
Binding Sites ◽  
Lectin Binding ◽  
Ventral Prostate ◽  
Rat Ventral Prostate ◽  
Lectin Binding Sites

Expression of androgen receptors and prostatic steroid-binding protein during development of the rat ventral prostate

1988 ◽  
Vol 117 (3) ◽  
pp. 361-NP ◽  
Author(s):  
Y. L. Zhang ◽  
Z. X. Zhou ◽  
Y. D. Zhang ◽  
M. G. Parker
Keyword(s):  
Gene Expression ◽  
Androgen Receptors ◽  
Binding Protein ◽  
Transient Increase ◽  
Neonatal Rat ◽  
Ventral Prostate ◽  
Rat Ventral Prostate ◽  
Old Rats ◽  
Steroid Binding ◽  
Steroid Binding Protein

ABSTRACT Prostatic steroid-binding protein (PSBP) mRNAs transcribed from the three genes C1, C2 and C3 were quantitated in neonatal rat ventral prostate by Northern blot analysis. Transcription was initiated at day 14 for C1 and C2 and day 10 for C3, and reached mature levels by day 21 for C1 and C2 and day 28 for C3. The changes of both cytoplasmic and nuclear prostatic androgen receptors in 10- to 150-day-old rats were investigated by radioligand assay and showed a fivefold transient increase between days 10 and 28. Thus there was a good correlation between the onset of PSBP gene expression and the transient increase in androgen receptors; increases in receptor concentration may be a prerequisite for changes in gene expression. J. Endocr. (1988) 117, 361–366

Synthetic progestins stimulate prostatic binding protein messenger RNAs in the rat ventral prostate

1990 ◽  
Vol 68 (2-3) ◽  
pp. 169-179 ◽  
Author(s):  
Claude Labrie ◽  
Jacques Simard ◽  
Hui-Fen Zhao ◽  
Georges Pelletier ◽  
Fernand Labrie
Keyword(s):  
Binding Protein ◽  
Ventral Prostate ◽  
Messenger Rnas ◽  
Rat Ventral Prostate

Localized matrix metalloproteinase (MMP)-2 and MMP-9 activity in the rat ventral prostate during the first week of postnatal development

2008 ◽  
Vol 129 (6) ◽  
pp. 805-815 ◽  
Author(s):  
Alexandre Bruni-Cardoso ◽  
Patrícia S. L. Vilamaior ◽  
Sebastião R. Taboga ◽  
Hernandes F. Carvalho
Keyword(s):  
Matrix Metalloproteinase ◽  
Postnatal Development ◽  
Ventral Prostate ◽  
Rat Ventral Prostate
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