Adult insect mushroom body neurons in primary culture: cell morphology and characterization of potassium channels

1998 ◽  
Vol 291 (3) ◽  
pp. 537-547 ◽  
Author(s):  
M. Cayre ◽  
S. D. Buckingham ◽  
A. Strambi ◽  
C. Strambi ◽  
D. B. Sattelle
Keyword(s):  
Potassium Channels ◽  
Primary Culture ◽  
Cell Morphology ◽  
Mushroom Body ◽  
Culture Cell ◽  
Primary Culture Cell ◽  
Adult Insect

Cold storage of biopsies from wild endangered native Chilean species in field conditions and subsequent isolation of primary culture cell lines

2008 ◽  
Vol 44 (8-9) ◽  
pp. 309-320 ◽  
Author(s):  
Heribelt Tovar ◽  
Felipe Navarrete ◽  
Lleretny Rodríguez ◽  
Oscar Skewes ◽  
Fidel Ovidio Castro
Keyword(s):  
Primary Culture ◽  
Cell Lines ◽  
Cold Storage ◽  
Culture Cell ◽  
Field Conditions ◽  
Primary Culture Cell

CALCITONIN GENE-RELATED PEPTIDE INCREASES K-ras EXPRESSION IN PRIMARY CULTURE CELL OF RAT SKELETAL MUSCLE

1996 ◽  
Vol 17 (2) ◽  
pp. 165-169 ◽  
Author(s):  
HIROAKI YOSHIKAWA ◽  
YOSHIHIRO YASUKAWA ◽  
SOICHI OKINO ◽  
MASAHARU TAKAMORI ◽  
KATSUHIRA IIDA
Keyword(s):  
Primary Culture ◽  
Calcitonin Gene Related Peptide ◽  
Culture Cell ◽  
Related Peptide ◽  
Primary Culture Cell ◽  
Calcitonin Gene

Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

1990 ◽  
Vol 48 (3) ◽  
pp. 416-417
Author(s):  
Jane K. Rosenthal ◽  
Dianne L. Atkins ◽  
William J. Marvin ◽  
Penny A. Krumm
Keyword(s):  
Cardiac Myocytes ◽  
Structural Changes ◽  
Three Dimensional ◽  
Adrenergic Innervation ◽  
Culture Cell ◽  
Serial Sections ◽  
Newborn Rats ◽  
Primary Culture Cell ◽  
Biological Studies ◽  
Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

Partial Characterization of a Fibrinolytic Inhibitor Produced by Cultured Endothelial Cells Derived from Human Umbilical Vein

1982 ◽  
Vol 47 (02) ◽  
pp. 128-131 ◽  
Author(s):  
F Esnard ◽  
E Dupuy ◽  
A M Dosne ◽  
E Bodevin
Keyword(s):  
Endothelial Cells ◽  
Primary Culture ◽  
Ammonium Sulphate ◽  
Concanavalin A ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Preliminary Characterization ◽  
Umbilical Vein Endothelial Cells ◽  
Ammonium Sulphate Saturation

SummaryA preliminary characterization of a fibrinolytic inhibitor released by human umbilical vein endothelial cells in primary culture is reported. This molecule of Mr comprised between 2 × 105 and 106 and of μ2 mobility precipitates at 43% ammonium sulphate saturation and is totally adsorbed on Concanavalin A Sepharose 4 B. A possible relationship with a macroglobulins is discussed.

scholarly journals Characterization of potassium channels in pancreatic β cells from ob /ob mice

FEBS Letters ◽  
1990 ◽  
Vol 266 (1-2) ◽  
pp. 105-108 ◽  
Author(s):  
Manuel Kukuljan ◽  
Min Yi Li ◽  
Illani Atwater
Keyword(s):  
Potassium Channels ◽  
Β Cells ◽  
Pancreatic Β Cells

scholarly journals Characterization of the liver-macrophages isolated from a mixed primary culture of neonatal swine hepatocytes

2014 ◽  
Vol 4 ◽  
pp. 1-7 ◽  
Author(s):  
Hiroshi Kitani ◽  
Miyako Yoshioka ◽  
Takato Takenouchi ◽  
Mitsuru Sato ◽  
Noriko Yamanaka
Keyword(s):  
Primary Culture ◽  
Liver Macrophages

scholarly journals Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae

2011 ◽  
Vol 43 (2) ◽  
pp. 83-90 ◽  
Author(s):  
G.F. Martins ◽  
B.A.M. Guedes ◽  
L.M. Silva ◽  
J.E. Serrão ◽  
C.L. Fortes-Dias ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Primary Culture ◽  
Morphological Characterization

Characterization of microglia isolated from a primary culture of embryonic rat brain

1990 ◽  
Vol 11 ◽  
pp. S136
Author(s):  
Makoto Hamanoue ◽  
Kazuyuki Nakajima ◽  
Nobuyuki Takei ◽  
Shinichi Kohsaka
Keyword(s):  
Rat Brain ◽  
Primary Culture
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