Expression of the gap junction protein connexin43 in the subependymal layer and the rostral migratory stream of the mouse: evidence for an inverse correlation between intensity of connexin43 expression and cell proliferation activity

1997 ◽  
Vol 287 (2) ◽  
pp. 243-253 ◽  
Author(s):  
Peter Albiez ◽  
Helga Bartels ◽  
Uwe de Vries ◽  
Rolf Dermietzel ◽  
Fernando Miragall
Keyword(s):  
Cell Proliferation ◽  
Gap Junction ◽  
Inverse Correlation ◽  
Rostral Migratory Stream ◽  
Junction Protein ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Gap Junction Protein ◽  
Migratory Stream ◽  
Subependymal Layer

scholarly journals Connexin 43 plays an important role in the transformation of human cholangiocytes upon stimulation withClonochis sinensisexcretory-secretory protein andN-nitrosodimethylamine

2018 ◽  
Author(s):  
Eun-Min Kim ◽  
Young Mee Bae ◽  
Min-Ho Choi ◽  
Sung-Tae Hong
Keyword(s):  
Cell Proliferation ◽  
Bile Duct ◽  
Gap Junction ◽  
Connexin 43 ◽  
Clonorchis Sinensis ◽  
Junction Protein ◽  
Gap Junction Protein ◽  
Cox 2 ◽  
Secretory Products ◽  
Cx 43

AbstractBackgroundClonorchis sinensisis a group I bio-carcinogen responsible for cholangiocarcinoma (CHCA) in humans. However, the mechanism by whichC. sinensispromotes carcinogenesis is unclear.MethodologyUsing the human cholangiocyte line H69, we investigated cell proliferation and gap junction protein expression after stimulation with the hepatotoxinN-nitrosodimethylamine (NDMA) and/or excretory-secretory products (ESP) of C.sinensis, which induce inflammation. NDMA and ESP treatment increased proliferation by 146% and the proportion of cells in the G2/M phase by 37%. Moreover, the expression of the cell cycle protein E2F1 and the cell proliferation-related proteins Ki-67 and cytokeratin 19 increased in response to combined treatment with NDMA and ESP. The gap-junction proteins connexin (Cx) 43 and Cx26 also increased. In contrast, Cx32 expression decreased in cells treated with NDMA and ESP. Cox-2 was also upregulated. Silencing of Cx43 reduced cell proliferation and significantly suppressed Cx26 and Cox-2 expression.ConclusionsThese results suggest that Cx43 is an important factor in CHCA induced byC. sinensisESP and NDMA and further investigations targeting this pathway may allow prevention of this deadly disease.Author summaryClonorchis sinensis, a human fluke, resides in the liver of humans and is commonly found in the common bile duct and gall bladder. This parasite is the main cause of cholangiocarcinoma, also called bile duct cancer, in humans. Of note, the excretory-secretory products (ESP) ofC. sinensisare known to cause inflammation in the biliary epithelium, which may ultimately result in neoplasms via production of reactive oxygen species and subsequent DNA damage. Together withN-nitrosodimethylamine (NDMA), a potent hepatotoxin that can cause fibrosis and tumors in the liver, ESP led to an increase in the growth and proliferation of cholangiocytes. Our results showed that the ESPs ofC. sinensisinduced pro-inflammatory responses by increasing the levels of proinflammatory cytokines and nuclear factor kappa B (NFκB), which in turn, enhanced the production of connexin 43 (Cx43), a gap-junction protein. Therefore, Cx 43 can serve as a potential target for developing a therapeutic strategy for the treatment of cholangiocarcinoma in humans.

Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein

FEBS Letters ◽  
1986 ◽  
Vol 205 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Roland Heynkes ◽  
Gisela Kozjek ◽  
Otto Traub ◽  
Klaus Willecke
Keyword(s):  
Gap Junction ◽  
Rat Liver ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Differential effect of subcellular localization of communication impairing gap junction protein connexin43 on tumor cell growth in vivo

Oncogene ◽  
2000 ◽  
Vol 19 (4) ◽  
pp. 505-513 ◽  
Author(s):  
V A Krutovskikh ◽  
S M Troyanovsky ◽  
C Piccoli ◽  
H Tsuda ◽  
M Asamoto ◽  
...  
Keyword(s):  
Cell Growth ◽  
Subcellular Localization ◽  
Tumor Cell ◽  
Gap Junction ◽  
Differential Effect ◽  
Tumor Cell Growth ◽  
Junction Protein ◽  
Gap Junction Protein

Cyclin D1 expression in astrocytomas is associated with cell proliferation activity and patient prognosis

1999 ◽  
Vol 188 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Satu-Leena Sallinen ◽  
Pauli K. Sallinen ◽  
Juha T. Kononen ◽  
Kirsi M. Syrj�koski ◽  
Nina N. Nupponen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cyclin D1 ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Patient Prognosis

scholarly journals Role of Gap Junction Protein Connexin43 in Astrogliosis Induced by Brain Injury

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47311 ◽  
Author(s):  
Nicolas Theodoric ◽  
John F. Bechberger ◽  
Christian C. Naus ◽  
Wun-Chey Sin
Keyword(s):  
Brain Injury ◽  
Gap Junction ◽  
Junction Protein ◽  
Gap Junction Protein
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