Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica

1994 ◽  
Vol 279 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Walter R. A. van Heumen ◽  
Gregg T. Nagle ◽  
Alexander Kurosky
Keyword(s):  
Aplysia Californica ◽  
Ultrastructural Localization ◽  
Egg Laying ◽  
Atrial Gland

Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica

1995 ◽  
Vol 279 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Walter R. A. van Heumen ◽  
Gregg T. Nagle ◽  
Alexander Kurosky
Keyword(s):  
Aplysia Californica ◽  
Ultrastructural Localization ◽  
Egg Laying ◽  
Atrial Gland

scholarly journals Evidence for the expression of three genes encoding homologous atrial gland peptides that cause egg laying in Aplysia.

1986 ◽  
Vol 261 (17) ◽  
pp. 7853-7859
Author(s):  
G T Nagle ◽  
S D Painter ◽  
J E Blankenship ◽  
J D Dixon ◽  
A Kurosky
Keyword(s):  
Egg Laying ◽  
Atrial Gland ◽  
Genes Encoding

Reproduction in Aplysia californica: correlations between egg laying in vivo and egg release in vitro

1980 ◽  
Vol 58 (11) ◽  
pp. 2163-2166 ◽  
Author(s):  
F. Edward Dudek ◽  
Amd Bonnie Soutar ◽  
Stephen S. Tobe
Keyword(s):  
Aplysia Californica ◽  
Egg Laying ◽  
Previous Episode ◽  
Laboratory Conditions ◽  
Release In Vitro

Aspects of egg laying by isolated Aplysia californica and egg release from ovotestis fragments were compared under laboratory conditions. The volume of eggs laid per episode increased as a function of time since the previous episode of egg laying. Egg output in vivo and egg release in vitro were maximal in autumn and minimal in spring, but a factor in the parietovisceral ganglion evoked egg release from ovotestis fragments throughout the year. These data are consistent with previous studies which have suggested that the effects of season and egg-laying history on egg laying involve substantial changes in the ovotestis.

scholarly journals Biochemical isolation and physiological identification of the egg-laying hormone in Aplysia californica.

1976 ◽  
Vol 68 (2) ◽  
pp. 197-210 ◽  
Author(s):  
S Arch ◽  
P Earley ◽  
T Smock
Keyword(s):  
Molecular Weight ◽  
Isoelectric Point ◽  
Gel Electrophoresis ◽  
Biological Function ◽  
Aplysia Californica ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Egg Laying ◽  
Separation Procedure ◽  
Bag Cells

It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone.

Atrial gland induction of the egg-laying response inAplysia californica

1978 ◽  
Vol 128 (1) ◽  
pp. 67-70 ◽  
Author(s):  
S. Arch ◽  
T. Smock ◽  
R. Gurvis ◽  
C. McCarthy
Keyword(s):  
Egg Laying ◽  
Atrial Gland

NEURONAL FEEDBACK IN EGG-LAYING BEHAVIOUR OF THE POND SNAIL LYMNAEA STAGNALIS

1993 ◽  
Vol 178 (1) ◽  
pp. 251-259 ◽  
Author(s):  
G. P. Ferguson ◽  
A. W. Pieneman ◽  
R. F. Jansen ◽  
A. Ter Maat
Keyword(s):  
Lymnaea Stagnalis ◽  
Aplysia Californica ◽  
Experimental System ◽  
Egg Laying ◽  
Neuroendocrine Cells ◽  
Behaviour Pattern ◽  
Pond Snail ◽  
Egg Laying Behaviour

The egg-laying behaviour of gastropod molluscs is controlled by peptidergic neuroendocrine cells and has provided an important experimental system for behavioural neurobiology. The genes that code for multiple peptides have been sequenced and the peptides themselves have been identified, thus enabling us to investigate how they act on the nervous system to produce the overt behavioural pattern (reviewed by Geraerts et al. 1988). The two animals that have been studied most extensively are the opisthobranch Aplysia californica and the pulmonate Lymnaea stagnalis. In both cases, the peptidergic neurones controlling egg laying are normally electrically silent (both in vivo and in vitro; Kupfermann, 1967; Pinsker and Dudek, 1977; Kits, 1980; Ter Maat et al. 1986) and produce multiple peptides (Rothman et al. 1983; Geraerts et al. 1985; Sigvardt et al. 1986), which are cleaved from a common protein precursor (Scheller et al. 1983; Vreugdenhil et al. 1988). Before egg laying, the cells produce a long-lasting discharge of action potentials (Pinsker and Dudek, 1977; Ter Maat et al. 1986). This electrical discharge initiates egg-laying behaviour, and during it the peptides (one of which initiates ovulation) are released into the blood. The demonstration, in Aplysia californica, that these peptides could have various effects on the activity of central neurones (reviewed by Mayeri and Rothman, 1985) led to the hypothesis that egg-laying behaviour is a neuroendocrine fixed action pattern controlled and coordinated by the concerted actions of the released peptides (Scheller and Axel, 1984). This hypothesis is also thought to apply to Lymnaea stagnalis (Vreugdenhil et al. 1988) because of the structural similarities between precursors of Aplysia californica and Lymnaea stagnalis egg-laying hormones. In this paper we investigate how the sequence of the various components of the egg-laying behaviour pattern is achieved.

Atrial gland cells synthesize a family of peptides that can induce egg laying inAplysia

1985 ◽  
Vol 156 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Gregg T. Nagle ◽  
Sherry D. Painter ◽  
Katrina L. Kelner ◽  
James E. Blankenship
Keyword(s):  
Egg Laying ◽  
Gland Cells ◽  
Atrial Gland

Purification and characterization of Aplysia atrial gland secretory granules containing egg-laying prohormone-related peptides

Peptides ◽  
1994 ◽  
Vol 15 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Gregg T. Nagle ◽  
Walter R.A. Van Heumen ◽  
Mahdy A. El-Hamzawy ◽  
Alexander Kurosky
Keyword(s):  
Secretory Granules ◽  
Egg Laying ◽  
Purification And Characterization ◽  
Atrial Gland

scholarly journals Polypeptide Secretion from the Isolated Parietovisceral Ganglion of Aplysia californica

1972 ◽  
Vol 59 (1) ◽  
pp. 47-59 ◽  
Author(s):  
S. Arch
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Aplysia Californica ◽  
Sodium Dodecyl ◽  
Egg Laying ◽  
High Potassium ◽  
Bathing Medium ◽  
Initial Exposure ◽  
Recipient Animal ◽  
Bag Cells

In vitro studies of the secretory behavior of the parietovisceral ganglion in Aplysia californica were performed. The aim of these studies was to investigate the release of polypeptides in response to depolarizing stimuli, and, in particular, to determine if a specific polypeptide known to induce egg laying in the intact animal is secreted into the bathing medium. During continuous perfusion of a ganglion preincubated in leucine-3H the application of either high-potassium medium or a burst of electrical stimuli (via the pleurovisceral connective nerve) evoked a marked increase in the amount of trichloroacetic acid (TCA)-precipitable radioactivity recovered in the perfusate. Enhanced release could be detected within 80 sec of the initial exposure to high potassium; however, incubation of a ganglion in calcium-free media before the application of high-potassium medium abolished the increase of precipitable radioactivity. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of perfusate samples revealed a significant change in the polypeptide species washed from the ganglion during high-potassium depolarization. Bioassays confirmed that egg laying is induced when high-potassium medium used to bathe a ganglion is injected into a recipient animal. These and other results permit the conclusion that the bulk of the polypeptide material secreted from the ganglion in response to depolarization is a specific neurohormone produced by two identified cell clusters, the so-called bag cells.

Sign in / Sign up

Export Citation Format

Share Document