Fine structure and lectin histochemistry of the apical surface of the free neuromast of Lampetra japonica

1994 ◽  
Vol 276 (2) ◽  
pp. 245-252
Author(s):  
Yukio Katori ◽  
Tomonori Takasaka ◽  
Makoto Ishikawa ◽  
Akira Tonosaki
Keyword(s):  
Fine Structure ◽  
Lectin Histochemistry ◽  
Apical Surface ◽  
Lampetra Japonica

Fine structure and lectin histochemistry of the apical surface of the free neuromast of Lampetra japonica

1994 ◽  
Vol 276 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Yukio Katori ◽  
Tomonori Takasaka ◽  
Makoto Ishikawa ◽  
Akira Tonosaki
Keyword(s):  
Fine Structure ◽  
Lectin Histochemistry ◽  
Apical Surface ◽  
Lampetra Japonica

A Study on the Fine Structure of the Lateral Line Organ of the Sea Eel

1973 ◽  
Vol 31 ◽  
pp. 648-649
Author(s):  
K. Hama
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Lateral Line ◽  
Low Frequency ◽  
Sensory Cells ◽  
Apical Surface ◽  
Voltage Electron ◽  
Sensory Epithelia ◽  
Low Frequency Vibration ◽  
Transmission Electron

The lateral line organs of the sea eel consist of canal and pit organs which are different in function. The former is a low frequency vibration detector whereas the latter functions as an ion receptor as well as a mechano receptor.The fine structure of the sensory epithelia of both organs were studied by means of ordinary transmission electron microscope, high voltage electron microscope and of surface scanning electron microscope.The sensory cells of the canal organ are polarized in front-caudal direction and those of the pit organ are polarized in dorso-ventral direction. The sensory epithelia of both organs have thinner surface coats compared to the surrounding ordinary epithelial cells, which have very thick fuzzy coatings on the apical surface.

Cytochemical and Morphological Differentiation of Epithelial Cells in Ductuli Efferentes of Chinese Hamster.

1970 ◽  
Vol 28 ◽  
pp. 316-317
Author(s):  
Masao Yokoyama ◽  
Jeffrey P. Chang
Keyword(s):  
Fine Structure ◽  
Chinese Hamster ◽  
Morphological Differentiation ◽  
Respiratory Epithelium ◽  
Apical Region ◽  
Apical Surface ◽  
Ciliated Cells ◽  
Ultrastructural Studies ◽  
Ductuli Efferentes ◽  
Adequate Data

Light microscopy and histochemistry of ductuli efferentes have been frequent subjects of investigation among various species of animals. Only a limited number of ultrastructural studies on this organ have been published. Adequate data on the fine structure and ultracytochemistry of ductuli efferentes of Chinese hamster are not available in the literature. Investigation of this subject therefore was initiated in this laboratory. Some preliminary findings are reported herein.Two distinctive types of cells, namely ciliated and nonciliated, are observed in the epithelia. The ciliated cells ('Fig. 1) always have typical kineto-cilia with 9 + 2 groups of microtubules. A few microvilli may be intermingled with the cilia on the apical surface of the plasma membrane. Also in the apical region, a peculiar structure (Fig. 1, arrow) resembling the “fibrogranular material” of respiratory epithelium is present. Its presence in the ductuli efferentes has not been reported previously.

scholarly journals Tissue-specific loss of fucosylated glycolipids in mice with targeted deletion of alpha(1,2)fucosyltransferase genes

2004 ◽  
Vol 380 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Masao IWAMORI ◽  
Steven E. DOMINO
Keyword(s):  
Enteric Bacteria ◽  
Lectin Histochemistry ◽  
Reproductive Organs ◽  
Apical Surface ◽  
Wild Type ◽  
Targeted Deletion ◽  
Ulex Europaeus ◽  
Intestinal Immune System ◽  
Ulex Europaeus Agglutinin I ◽  
Female Reproductive Organs

Glycolipids in epithelial tissues of the gastrointestinal tract act as receptors for enteric bacteria and are implicated in the activation of the intestinal immune system. To clarify the genes involved in the fucosylation of the major glycolipids, substrate glycolipids and fucosylated products were measured in tissues of wild-type and mutant mice lacking α(1,2)fucosyltransferase genes FUT1 or FUT2. Quantitative determination was performed by TLC-immunostaining for GA1 (Gg4Cer), FGA1 (fucosyl GA1), GM1 (II3NeuAc-Gg4Cer), FGM1 (fucosyl GM1), and Forssman glycolipids. Both FGM1 and FGA1 completely disappeared from the antrum, cecum, and colon of FUT2-null mice, but not those of FUT1-null and wild-type mice. Precursor glycolipids, GM1 and GA1, accumulated in tissues of FUT2-null mice, indicating that the FUT2-encoded enzyme preferentially participates in the fucosylation of GA1 and GM1 in these tissues. Female reproductive organs were similarly found to utilize FUT2 for the fucosylation of glycolipids FGA1 (uterus and cervix), and FGM1 (ovary), due to their absence in FUT2-null mice. In FUT1-null mice FGA1 was lost from the pancreas, but was present in wild-type and FUT2-null mice, indicating that FUT1 is essential for fucosylation of GA1 in the pancreas. Ulex europaeus agglutinin-I lectin histochemistry for α(1,2)fucose residues confirmed the absence of α(1,2)fucose residues from the apical surface of pancreatic acinar glands of FUT1-null mice. Ileum, epididymis, and testis retained specific fucosylated glycolipids, irrespective of targeted deletion of either gene, indicating either compensation for or redundancy of the α(1,2)fucosyltransferase genes in these tissues.

Fine Structure of the Integument of the Cryptocotyle Lingua Redia

1967 ◽  
Vol 25 ◽  
pp. 218-219
Author(s):  
P. L. Krupa ◽  
A. K. Bal ◽  
G. H. Cousineau
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Body Wall ◽  
Sea Water ◽  
Snail Host ◽  
Littorina Littorea ◽  
Apical Surface ◽  
Usual Manner ◽  
Flat Structures ◽  
Biochemical Studies

Recent electron microscope studies of trematode rediae have revealed microvilli projecting from the integument, presumably to facilitate exchange of materials between the parasite and the snail host. This report on the redia of Cryptocotyle lingua extends these observations and provides an additional basis for future biochemical studies of host-trematode relations. The rediae were removed from crushed snails (Littorina littorea), washed in filtered sea water, fixed in 5% glutaraldehyde-paraformaldehyde solution in sea water, postosmicated, and embedded in Epon in the usual manner.Light microscopy shows that the apical surface of the redial body wall has numerous undulations or corrugations of varying dimensions and shapes (Fig. 1, arrow). As seen in the electron microscope, many cytoplasmic projections or microvilli stem perpendicularly from these crests and valleys (Figs. 2,3). In tangential sections of the redial surface (Fig. 4), the microvilli appear as thin, flat structures or spatulae, rather than as cylindrical, digitiform projections. The expanded surface of some microvilli is also suggested in sagittal- sectioned rediae (Fig. 2).

Fine structure of the retinal pigment epithelium in the mud minnow (Umbra limi)

1980 ◽  
Vol 58 (2) ◽  
pp. 258-265 ◽  
Author(s):  
C. R. Braekevelt
Keyword(s):  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Epithelial Cells ◽  
Smooth Endoplasmic Reticulum ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Apical Surface ◽  
Phagocytic Cells ◽  
Bruch's Membrane ◽  
Bruch’S Membrane

The fine structure of the retinal pigment epithelial layer and associated regions has been studied by electron microscopy in the adult mud minnow Umbra limi. The pigment epithelium is composed of a single layer of large columnar cells. Each epithelial cell has abundant small mitochondria, much smooth endoplasmic reticulum (often in highly organized arrays), myeloid bodies, phagosomes and pigment granules. Rough endoplasmic reticulum and ribosomes are scarce. The scleral or basal border of the epithelial cells is but minimally infolded whereas the vitreal or apical surface displays numerous elongated processes which surround the inner and outer segments of the photoreceptors. Unattached, presumably phagocytic cells are a constant feature both between the retinal epithelial cells and within Bruch's membrane. Bruch's membrane lacks a central elastic layer and is composed only of three layers. The endothelial wall of the choriocapillaris bordering Bruch's membrane is typically very thin with a few fenestrations. This region of the mud minnow eye is morphologically similar to that described in other teleost species but differs from that described in most mammals.

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

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