Taxol impairs anterograde axonal transport of microinjected horseradish peroxidase in dorsal root ganglia neurons in vitro

2000 ◽  
Vol 299 (2) ◽  
pp. 213-224 ◽  
Author(s):  
Carsten Theiss ◽  
Karl Meller
Keyword(s):  
Horseradish Peroxidase ◽  
Axonal Transport ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Anterograde Axonal Transport ◽  
Dorsal Root Ganglia Neurons

Importance of monovalent ions for the fast axonal transport of proteins

1981 ◽  
Vol 59 (1) ◽  
pp. 31-36 ◽  
Author(s):  
P.-A. Lavoie
Keyword(s):  
Axonal Transport ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Time Course ◽  
Protein Transport ◽  
Choline Chloride ◽  
Fast Axonal Transport ◽  
Spinal Nerves ◽  
Monovalent Ions

Proteins labeled with [35S] methionine or [3H]leucine were generated in vitro in bullfrog dorsal root ganglia and their fast axonal transport in the spinal nerves was followed during a subsequent incubation period. Incubation of the ganglia in a medium where sucrose, choline chloride, or sodium isethionate replaced NaCl caused respectively an 88, a 37, or a 76% reduction in the quantity of proteins carried by the fast axonal transport system; no decrease in synthesis of labeled proteins was observed and protein transport followed the usual time course. Incubation of desheathed spinal nerves in a medium where sucrose replaced NaCl reduced by 67% the quantity of labeled proteins which were transported past the desheathed region. Although both the axons and the dorsal root ganglia exhibit the requirement for monovalent ions to maintain fast axonal transport, the possibility that the ionic requirements of the ganglia pertain to the somal portion of the nerve cell is discussed.

scholarly journals Neurotrophic effects of GM1 ganglioside, NGF, and FGF2 on canine dorsal root ganglia neurons in vitro

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
S. Schwarz ◽  
A. Lehmbecker ◽  
W. Tongtako ◽  
K. Hahn ◽  
Y. Wang ◽  
...  
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Gm1 Ganglioside ◽  
Dorsal Root Ganglia Neurons

scholarly journals Hydrogen Peroxide Induces Muscle Nociception via Transient Receptor Potential Ankyrin 1 Receptors

2017 ◽  
Vol 127 (4) ◽  
pp. 695-708 ◽  
Author(s):  
Daisuke Sugiyama ◽  
Sinyoung Kang ◽  
Nicholas Arpey ◽  
Preeyaphan Arunakul ◽  
Yuriy M. Usachev ◽  
...  
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Deep Muscle ◽  
Plantar Incision ◽  
Dorsal Root Ganglia Neurons ◽  
Transient Receptor

Abstract Background H2O2 has a variety of actions in skin wounds but has been rarely studied in deep muscle tissue. Based on response to the transient receptor potential ankyrin 1 antagonists after plantar incision, we hypothesized that H2O2 exerts nociceptive effects via the transient receptor potential ankyrin 1 in muscle. Methods Nociceptive behaviors in rats (n = 269) and mice (n = 16) were evaluated after various concentrations and volumes of H2O2 were injected into the gastrocnemius muscle or subcutaneous tissue. The effects of H2O2 on in vivo spinal dorsal horn neuronal activity and lumbar dorsal root ganglia neurons in vitro were evaluated from 26 rats and 6 mice. Results Intramuscular (mean ± SD: 1,436 ± 513 s) but not subcutaneous (40 ± 58 s) injection of H2O2 (100 mM, 0.6 ml) increased nociceptive time. Conditioned place aversion was evident after intramuscular (–143 ± 81 s) but not subcutaneous (–2 ± 111 s) injection of H2O2. These H2O2-induced behaviors were blocked by transient receptor potential ankyrin 1 antagonists. Intramuscular injection of H2O2 caused sustained in vivo activity of dorsal horn neurons, and H2O2 activated a subset of dorsal root ganglia neurons in vitro. Capsaicin nerve block decreased guarding after plantar incision and reduced nociceptive time after intramuscular H2O2. Nociceptive time after intramuscular H2O2 in transient receptor potential ankyrin 1 knockout mice was shorter (173 ± 156 s) compared with wild-type mice (931 ± 629 s). Conclusions The greater response of muscle tissue to H2O2 may help explain why incision that includes deep muscle but not skin incision alone produces spontaneous activity in nociceptive pathways.

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