Characterization of double minute chromosomes' DNA content in a human high grade astrocytoma cell line by using comparative genomic hybridization and fluorescence in situ hybridization

1996 ◽  
Vol 98 (3) ◽  
pp. 265-270 ◽  
Author(s):  
Michel Giollant ◽  
Suzanne Bertrand ◽  
Pierre Verrelle ◽  
Stanislas du Manoir ◽  
Thomas Ried ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Content ◽  
Comparative Genomic ◽  
High Grade ◽  
High Grade Astrocytoma ◽  
Astrocytoma Cell ◽  
Double Minute ◽  
Double Minute Chromosomes

scholarly journals FLT3 Amplification as Double Minute Chromosomes in a Patient with Chronic Myelomonocytic Leukemia

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Heyang Zhang ◽  
Xiaoxue Wang ◽  
Shibo Li ◽  
Xianfu Wang ◽  
Xianglan Lu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Gene Amplification ◽  
Chronic Myelomonocytic Leukemia ◽  
Comparative Genomic ◽  
Myeloid Neoplasms ◽  
Double Minute ◽  
Myelomonocytic Leukemia ◽  
Double Minute Chromosomes

Double minute chromosomes (dmins) are a form of gene amplification presenting as small spherical paired chromatin bodies. Dmins are rare in hematologic malignancies and are generally associated with a poor prognosis. Some case reports identified MYC or MLL gene amplification performing as dmin in myeloid neoplasms. FLT3 (FMS-related tyrosine kinase 3) acts as an oncogene in myeloid neoplasms which is associated with several signal transduction pathways. Genomic amplification of FLT3 has not been reported in hematological disease. The current study attempts to demonstrate the existence of double minute chromosomes via FLT3 gene amplification in a patient diagnosed with chronic myelomonocytic leukemia (CMML). Routine G-banded karyotype, array-based comparative genomic hybridization, and fluorescence in situ hybridization analyses were used to characterize the cytogenetic abnormality in the patient’s bone marrow. FLT3 amplification as dmins in a patient with CMML was revealed. This case study reports a rare double minute chromosome via FLT3 amplification in CMML by using array-based comparative genomic hybridization and fluorescence in situ hybridization analyses. The study also proposed another possible mechanism of FLT3 genes in leukemogenesis.

Sign in / Sign up

Export Citation Format

Share Document