Tagged Transcriptome Display (TTD) in indica rice using Ac transposition

2001 ◽  
Vol 266 (1) ◽  
pp. 1-11 ◽  
Author(s):  
A. Kohli ◽  
J. Xiong ◽  
R. Greco ◽  
P. Christou ◽  
A. Pereira
Keyword(s):  
Indica Rice ◽  
Ac Transposition

scholarly journals Improved Transformation and Regeneration of Indica Rice: Disruption of SUB1A as a Test Case via CRISPR-Cas9

2021 ◽  
Vol 22 (13) ◽  
pp. 6989
Author(s):  
Yuya Liang ◽  
Sudip Biswas ◽  
Backki Kim ◽  
Julia Bailey-Serres ◽  
Endang M. Septiningsih
Keyword(s):  
Gene Editing ◽  
Indica Rice ◽  
Crop Improvement ◽  
Immature Embryo ◽  
Test Case ◽  
Submergence Tolerance ◽  
Embryogenic Calli ◽  
Submergence Stress ◽  
Efficient Vector ◽  
Vector Delivery

Gene editing by use of clustered regularly interspaced short palindromic repeats (CRISPR) has become a powerful tool for crop improvement. However, a common bottleneck in the application of this approach to grain crops, including rice (Oryza sativa), is efficient vector delivery and calli regeneration, which can be hampered by genotype-dependent requirements for plant regeneration. Here, methods for Agrobacterium-mediated and biolistic transformation and regeneration of indica rice were optimized using CRISPR-Cas9 gene-editing of the submergence tolerance regulator SUBMERGENCE 1A-1 gene of the cultivar Ciherang-Sub1. Callus induction and plantlet regeneration methods were optimized for embryogenic calli derived from immature embryos and mature seed-derived calli. Optimized regeneration (95%) and maximal editing efficiency (100%) were obtained from the immature embryo-derived calli. Phenotyping of T1 seeds derived from the edited T0 plants under submergence stress demonstrated inferior phenotype compared to their controls, which phenotypically validates the disruption of SUB1A-1 function. The methods pave the way for rapid CRISPR-Cas9 gene editing of recalcitrant indica rice cultivars.

scholarly journals Effects of different mechanical direct seeding methods on grain yield and lodging resistance of early indica rice in South China

2021 ◽  
Vol 20 (5) ◽  
pp. 1204-1215
Author(s):  
Wen-xia WANG ◽  
Jie DU ◽  
Yan-zhi ZHOU ◽  
Yong-jun ZENG ◽  
Xue-ming TAN ◽  
...  
Keyword(s):  
Grain Yield ◽  
South China ◽  
Indica Rice ◽  
Direct Seeding ◽  
Lodging Resistance ◽  
Early Indica Rice

scholarly journals Transposition Pattern of the Maize Element Ac from the Bz-M2(ac) Allele.

Genetics ◽  
1989 ◽  
Vol 122 (2) ◽  
pp. 447-457 ◽  
Author(s):  
H K Dooner ◽  
A Belachew
Keyword(s):  
Donor Site ◽  
Receptor Site ◽  
Receptor Sites ◽  
Mutable Allele ◽  
Ac Transposition ◽  
P Locus

Abstract The pattern of transposition of Ac from the mutable allele bz-m2(Ac) has been investigated. Stable (bz-s) and finely spotted (bz-m(F)) exceptions were selected from coarsely spotted bz-m2(Ac) ears. The presence or absence of a transposed Ac (trAc) in the genome was determined and, when present, the location of the trAc was mapped relative to the flanking markers sh and wx. The salient general features of Ac transposition to sites linked to bz are that the receptor sites tend to be clustered on either side of the bz donor site and that transposition is bidirectional and nonpolar. Thus, the symmetrical clustering in the distribution of receptor sites adjacent to bz differs from the asymmetrical clustering reported in 1984 for the P locus by I. M. GREENBLATT. Though Ac tends to transpose preferentially to closely linked sites, an appreciable fraction of Ac transpositions from bz-m2(Ac) is to unlinked sites: 41% among bz-s derivatives and 59% among bz-m(F) derivatives. Many transposition events among the bz-m(F) selections result in kernels carrying a genetically noncorresponding embryo. These can be interpreted as twin sectors arising at one of the megagametophytic mitoses. The bz locus data fit the earlier (1962) model of I. M. GREENBLATT and R. A. BRINK in which transposition takes place from a replicated donor site to either an unreplicated or replicated receptor site.

scholarly journals Improved Method for Anther Culture of an Indica Rice Cultivar of Thailand

2000 ◽  
Vol 3 (3) ◽  
pp. 254-256 ◽  
Author(s):  
Prapa Sripichitt ◽  
Takashi Ozawa ◽  
Motoyasu Otani ◽  
Takiko Shimada
Keyword(s):  
Anther Culture ◽  
Indica Rice ◽  
Rice Cultivar ◽  
Improved Method ◽  
Indica Rice Cultivar
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