Pim1, a MAP kinase involved in cell wall integrity in Pichia pastoris

2001 ◽  
Vol 265 (4) ◽  
pp. 604-614 ◽  
Author(s):  
I.C. Cosano ◽  
H. Martín ◽  
M. Flández ◽  
C. Nombela ◽  
M. Molina
Keyword(s):  
Cell Wall ◽  
Pichia Pastoris ◽  
Map Kinase ◽  
Cell Wall Integrity

The MpkA MAP kinase module regulates cell wall integrity signaling and pyomelanin formation in Aspergillus fumigatus

2009 ◽  
Vol 46 (12) ◽  
pp. 909-918 ◽  
Author(s):  
Vito Valiante ◽  
Radhika Jain ◽  
Thorsten Heinekamp ◽  
Axel A. Brakhage
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Map Kinase ◽  
Cell Wall Integrity

scholarly journals Geranylgeranyltransferase Cwg2-Rho4/Rho5 module is implicated in the Pmk1 MAP kinase-mediated cell wall integrity pathway in fission yeast

2015 ◽  
Vol 20 (4) ◽  
pp. 310-323 ◽  
Author(s):  
Akira Doi ◽  
Ayako Kita ◽  
Yuki Kanda ◽  
Takaya Uno ◽  
Keita Asami ◽  
...  
Keyword(s):  
Cell Wall ◽  
Map Kinase ◽  
Fission Yeast ◽  
Cell Wall Integrity ◽  
Cell Wall Integrity Pathway

scholarly journals PRO40 Is a Scaffold Protein of the Cell Wall Integrity Pathway, Linking the MAP Kinase Module to the Upstream Activator Protein Kinase C

PLoS Genetics ◽  
2014 ◽  
Vol 10 (9) ◽  
pp. e1004582 ◽  
Author(s):  
Ines Teichert ◽  
Eva Katharina Steffens ◽  
Nicole Schnaß ◽  
Benjamin Fränzel ◽  
Christoph Krisp ◽  
...  
Keyword(s):  
Cell Wall ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Map Kinase ◽  
Scaffold Protein ◽  
Cell Wall Integrity ◽  
Activator Protein ◽  
Kinase C ◽  
Cell Wall Integrity Pathway

scholarly journals The interaction of Slt2 MAP kinase with Knr4 is necessary for signalling through the cell wall integrity pathway in Saccharomyces cerevisiae

2003 ◽  
Vol 49 (1) ◽  
pp. 23-35 ◽  
Author(s):  
Helene Martin-Yken ◽  
Adilia Dagkessamanskaia ◽  
Fadi Basmaji ◽  
Arnaud Lagorce ◽  
Jean Francois
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Map Kinase ◽  
Cell Wall Integrity ◽  
Cell Wall Integrity Pathway

scholarly journals A network of MAP-Kinase pathways and transcription factors regulates cell-to-cell communication and cell wall integrity in Neurospora crassa

2018 ◽  
Author(s):  
Monika S. Fischer ◽  
Vincent W. Wu ◽  
Ji E. Lee ◽  
Ronan C. O’Malley ◽  
N. Louise Glass
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Map Kinase ◽  
Neurospora Crassa ◽  
Cell Fusion ◽  
Regulatory Network ◽  
Cell Communication ◽  
Cell Wall Integrity ◽  
Cell Integrity ◽  
Map Kinase Pathways

ABSTRACTMaintenance of cell integrity and cell-to-cell communication are fundamental biological processes. Filamentous fungi, such as Neurospora crassa, depend on communication to locate compatible cells, coordinate cell fusion, and establish a robust hyphal network. Two MAP-Kinase pathways are essential for communication and cell fusion in N. crassa; the Cell Wall Integrity/MAK-1 pathway and the MAK-2 (signal response) pathway. Previous studies have demonstrated several points of cross talk between the MAK-1 and MAK-2 pathways, which is likely necessary for oordinating chemotropic growth toward an extracellular signal, and then mediating cell fusion. Canonical MAP-Kinase pathways begin with signal reception and end with a transcriptional response. Two transcription factors, ADV-1 and PP-1, are essential for communication and cell fusion. PP-1 is the conserved target of MAK-2, while it is unclear what targets ADV-1. We did RNAseq on Δadv-1, Δpp-1, and wild-type cells and found that ADV-1 and PP-1 have a shared regulon including many genes required for communication, cell fusion, growth, development, and stress response. We identified ADV-1 and PP-1 binding sites across the genome by adapting the in vitro method of DNA-Affinity Purification sequencing (DAP-seq) for N. crassa. To elucidate the regulatory network, we misexpressed each transcription factor in each upstream MAPK deletion mutant. Misexpression of adv-1 was sufficient to fully suppress the phenotype of the Δpp-1 mutant and partially suppress the phenotype of the Δmak-1 mutant. Collectively, our data demonstrate that the MAK-1-ADV-1 and MAK-2- PP-1 pathways form a tight regulatory network that maintains cell integrity and mediates communication and cell fusion.

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