Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance

1999 ◽  
Vol 262 (4-5) ◽  
pp. 876-883 ◽  
Author(s):  
B. Wackwitz ◽  
J. Bongaerts ◽  
S. D. Goodman ◽  
G. Unden
Keyword(s):  
Escherichia Coli ◽  
Growth Phase ◽  
Binding Sites ◽  
Expression In Escherichia Coli ◽  
K 12

scholarly journals The c-Type Cytochrome OmcA Localizes to the Outer Membrane upon Heterologous Expression in Escherichia coli

2008 ◽  
Vol 190 (14) ◽  
pp. 5127-5131 ◽  
Author(s):  
James W. Donald ◽  
Matthew G. Hicks ◽  
David J. Richardson ◽  
Tracy Palmer
Keyword(s):  
Escherichia Coli ◽  
Outer Membrane ◽  
Shewanella Oneidensis ◽  
Type Ii Secretion ◽  
Type Ii ◽  
E Coli ◽  
Expression In Escherichia Coli ◽  
Type Ii Secretion System ◽  
Surface Localization ◽  
K 12

ABSTRACT We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

scholarly journals Metabolic regulation of Irp gene expression in Escherichia coli K-12

Microbiology ◽  
1997 ◽  
Vol 143 (6) ◽  
pp. 2079-2084 ◽  
Author(s):  
C. F. Chen ◽  
J. Lan ◽  
M. Korovine ◽  
Z. Q. Shao ◽  
L. Tao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Metabolic Regulation ◽  
Expression In Escherichia Coli ◽  
K 12

scholarly journals The Putrescine Importer PuuP of Escherichia coli K-12

2009 ◽  
Vol 191 (8) ◽  
pp. 2776-2782 ◽  
Author(s):  
Shin Kurihara ◽  
Yuichi Tsuboi ◽  
Shinpei Oda ◽  
Hyeon Guk Kim ◽  
Hidehiko Kumagai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cluster ◽  
Growth Phase ◽  
Exponential Growth ◽  
Minimal Medium ◽  
Exponential Growth Phase ◽  
E Coli ◽  
Genes Encoding ◽  
K 12 ◽  
Utilization Pathway

ABSTRACT The Puu pathway is a putrescine utilization pathway involving gamma-glutamyl intermediates. The genes encoding the enzymes of the Puu pathway form a gene cluster, the puu gene cluster, and puuP is one of the genes in this cluster. In Escherichia coli, three putrescine importers, PotFGHI, PotABCD, and PotE, were discovered in the 1990s and have been studied; however, PuuP had not been discovered previously. This paper shows that PuuP is a novel putrescine importer whose kinetic parameters are equivalent to those of the polyamine importers discovered previously. A puuP + strain absorbed up to 5 mM putrescine from the medium, but a ΔpuuP strain did not. E. coli strain MA261 has been used in previous studies of polyamine transporters, but PuuP had not been identified previously. It was revealed that the puuP gene of MA261 was inactivated by a point mutation. When E. coli was grown on minimal medium supplemented with putrescine as the sole carbon or nitrogen source, only PuuP among the polyamine importers was required. puuP was expressed strongly when putrescine was added to the medium or when the puuR gene, which encodes a putative repressor, was deleted. When E. coli was grown in M9-tryptone medium, PuuP was expressed mainly in the exponential growth phase, and PotFGHI was expressed independently of the growth phase.

scholarly journals Substitutions at Auxiliary Operator O3 Enhance Repression by Nitrate-Responsive Regulator NarL at Synthetic lac Control Regions in Escherichia coli K-12

2007 ◽  
Vol 190 (1) ◽  
pp. 428-433 ◽  
Author(s):  
Valley Stewart ◽  
Peggy J. Bledsoe
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Higher Order ◽  
Cooperative Interactions ◽  
K 12 ◽  
Control Regions

ABSTRACT We constructed monocopy lac operon control regions in which the operators O1-lac and O3-lac were replaced by NarL and NarP binding sites from the nirB or napF operon control regions. The results support the hypothesis that DNA-bound dimers of phospho-NarL can participate in higher-order cooperative interactions.

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