A plasmid-based vector system for the cloning and expression of Helicobacter pylori genes encoding outer membrane proteins

1999 ◽  
Vol 262 (3) ◽  
pp. 501-507 ◽  
Author(s):  
W. Fischer ◽  
D. Schwan ◽  
E. Gerland ◽  
G. E. Erlenfeld ◽  
S. Odenbreit ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Cloning And Expression ◽  
Vector System ◽  
Genes Encoding

scholarly journals Selective pressure on membrane proteins drives the evolution of Helicobacter pylori Colombian subpopulations

2021 ◽  
Author(s):  
Alix Andrea Guevara Tique ◽  
Roberto C. Torres ◽  
Fabian Leonardo Castro Valencia ◽  
John Jairo Suárez ◽  
Ángel Alexandro Criollo Rayo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Population Structure ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Selective Pressure ◽  
Outer Membrane Proteins ◽  
Geographic Origin ◽  
Population Structure Analysis ◽  
Genes Encoding ◽  
H Pylori

Helicobacter pylori have coevolved with mankind since its origins, adapting to different human groups. In America H. pylori has evolved in several subpopulations specific for regions or even countries. In this study we analyzed the genome of 163 Colombian strains along with 1,113 strains that represent worldwide H. pylori populations to better discern the ancestry and adaption to Colombian people. Population structure was inferred with FineStructure and chromosome painting identifying the proportion of ancestries in Colombian isolates. Phylogenetic relationship was analyzed using the SNPs present in the core genome. Also, a Fst analysis was done to identify the gene variants with the strongest fixation in the identified Colombian subpopulations in relation to their parent population hspSWEurope. Worldwide, population structure analysis allowed the identification of two Colombian subpopulations, the previously described hspSWEuropeColombia and a novel subpopulation named hspColombia. In addition, three subgroups of H. pylori were identified within hspColombia that follow their geographic origin. The Colombian H. pylori subpopulations represent an admixture of European, African and Native indigenous ancestry; although some genomes showed a high proportion of self-identity, suggesting a strong adaption to these mestizo Colombian groups. The Fst analysis identified 82 SNPs significantly fixed in 26 genes of the hspColombia subpopulation that encode mainly for outer membrane proteins and proteins involved in central metabolism. The strongest fixation indices were identified in genes encoding the membrane proteins HofC, HopE, FrpB-4 and Sialidase A. These findings demonstrate that H. pylori has evolved in Colombia to give rise to subpopulations following a geographical structure, evolving to an autochthonous genetic pool, drive by a positive selective pressure especially on genes encoding for outer membrane proteins.

scholarly journals Proteins Released by Helicobacter pylori In Vitro

2002 ◽  
Vol 184 (22) ◽  
pp. 6155-6162 ◽  
Author(s):  
Nayoung Kim ◽  
David L. Weeks ◽  
Jai Moo Shin ◽  
David R. Scott ◽  
Mary K. Young ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Membrane Proteins ◽  
Dna Binding ◽  
Outer Membrane ◽  
Nalidixic Acid ◽  
Outer Membrane Proteins ◽  
Gastric Epithelium ◽  
Synthesis Inhibitor ◽  
Vitro Analysis

ABSTRACT Secretion of proteins by Helicobacter pylori may contribute to gastric inflammation and epithelial damage. An in vitro analysis was designed to identify proteins released by mechanisms other than nonspecific lysis. The radioactivity of proteins in the supernatant was compared with that of the intact organism by two-dimensional gel phosphorimaging following a 4-h pulse-chase. The ratio of the amount of UreB, a known cytoplasmic protein, in the supernatant to that in the pellet was found to be 0.25, and this was taken as an index of lysis during the experiments (n = 6). Ratios greater than that of UreB were used to distinguish proteins that were selectively released into the medium. Thus, proteins enriched more than 10-fold in the supernatant compared to UreB were identified by mass spectrometry. Sixteen such proteins were present in the supernatant: VacA; a conserved secreted protein (HP1286); putative peptidyl cis-trans isomerase (HP0175); six proteins encoded by HP0305, HP0231, HP0973, HP0721, HP0129, and HP0902; thioredoxin (HP1458); single-stranded-DNA-binding 12RNP2 precursor (HP0827); histone-like DNA-binding protein HU (HP0835); ribosomal protein L11 (HP1202); a putative outer membrane protein (HP1564); and outer membrane proteins Omp21 (HP0913) and Omp20 (HP0912). All except HP0902, thioredoxin, HP0827, HP0835, and HP1202 had a signal peptide. When nalidixic acid, a DNA synthesis inhibitor, was added to inhibit cell division but not protein synthesis, to decrease possible contamination due to outer membrane shedding, two outer membrane proteins (Omp21 and Omp20) disappeared from the supernatant, and the amount of VacA also decreased. Thus, 13 proteins were still enriched greater than 10-fold in the medium after nalidixic acid treatment, suggesting these were released specifically, possibly by secretion. These proteins may be implicated in H. pylori-induced effects on the gastric epithelium.

Gene conversions in genes encoding outer-membrane proteins in H. pylori and C. pneumoniae

2001 ◽  
Vol 17 (1) ◽  
pp. 7-10 ◽  
Author(s):  
I.King Jordan ◽  
Kira S Makarova ◽  
Yuri I Wolf ◽  
Eugene V Koonin
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Genes Encoding ◽  
H Pylori ◽  
Gene Conversions

Helicobacter pylori infection in mice: Role of outer membrane proteins in colonization and inflammation

2002 ◽  
Vol 123 (6) ◽  
pp. 1992-2004 ◽  
Author(s):  
Yoshio Yamaoka ◽  
Masakazu Kita ◽  
Tadashi Kodama ◽  
Shigeyoshi Imamura ◽  
Tomoyuki Ohno ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Helicobacter Pylori Infection

scholarly journals Analysis of Surface-Exposed Outer Membrane Proteins in Helicobacter pylori

2014 ◽  
Vol 196 (13) ◽  
pp. 2455-2471 ◽  
Author(s):  
B. J. Voss ◽  
J. A. Gaddy ◽  
W. H. McDonald ◽  
T. L. Cover
Keyword(s):  
Helicobacter Pylori ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins

scholarly journals Genome-wide mutation analysis of Helicobacter pylori after inoculation to Mongolian gerbils

Gut Pathogens ◽  
2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Rumiko Suzuki ◽  
Kazuhito Satou ◽  
Akino Shiroma ◽  
Makiko Shimoji ◽  
Kuniko Teruya ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Human Stomach ◽  
Next Generation Sequencing Data ◽  
Mongolian Gerbils ◽  
New Host ◽  
Model Animal ◽  
H Pylori

Abstract Background Helicobacter pylori is a pathogenic bacterium that causes various gastrointestinal diseases in the human stomach. H. pylori is well adapted to the human stomach but does not easily infect other animals. As a model animal, Mongolian gerbils are often used, however, the genome of the inoculated H. pylori may accumulate mutations to adapt to the new host. To investigate mutations occurring in H. pylori after infection in Mongolian gerbils, we compared the whole genome sequence of TN2 wild type strain (TN2wt) and next generation sequencing data of retrieved strains from the animals after different lengths of infection. Results We identified mutations in 21 loci of 17 genes of the post-inoculation strains. Of the 17 genes, five were outer membrane proteins that potentially influence on the colonization and inflammation. Missense and nonsense mutations were observed in 15 and 6 loci, respectively. Multiple mutations were observed in three genes. Mutated genes included babA, tlpB, and gltS, which are known to be associated with adaptation to murine. Other mutations were involved with chemoreceptor, pH regulator, and outer membrane proteins, which also have potential to influence on the adaptation to the new host. Conclusions We confirmed mutations in genes previously reported to be associated with adaptation to Mongolian gerbils. We also listed up genes that mutated during the infection to the gerbils, though it needs experiments to prove the influence on adaptation.

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