The gene for mitochondrial ribosomal protein S14 has been transferred to the nucleus in Arabidopsis thaliana

1999 ◽  
Vol 262 (1) ◽  
pp. 139-144 ◽  
Author(s):  
P. Figueroa ◽  
I. Gómez ◽  
R. Carmona ◽  
L. Holuigue ◽  
A. Araya ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Ribosomal Protein ◽  
Mitochondrial Ribosomal Protein

Characterization of Arabidopsis thaliana lines with T-DNA insertions in the mitochondrial ribosomal protein genes Rps14 and Rps19

2019 ◽  
Vol 79 (02) ◽  
Author(s):  
Suman Lata ◽  
Anshul Watts ◽  
S. R. Bhat
Keyword(s):  
Arabidopsis Thaliana ◽  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Ribosomal Protein Genes ◽  
Protein Coding ◽  
Mitochondrial Ribosomal Protein ◽  
Genes Encoding ◽  
Coordinated Expression ◽  
Dna Insertion ◽  
Mutant Lines

In Arabidopsis, most of the genes encoding mitochondrial ribosomal proteins are located in the nucleus and only seven are present in the mitochondrial genome. Assembly of a functional ribosome requires coordinated expression of ribosomal protein encoding genes located in both these organelles. Genes and promoters of nuclear encoded mitochondrial ribosomal protein coding genes of plants have not been well characterized so far. In the present study we have characterized Arabidopsis thaliana SALK mutant lines with T-DNA insertion in Rps14 or Rps19 gene. The location of T-DNA insertion in the mutant lines was confirmed and plants homozygous and hemizygous for TDNA insertion were identified for both Rps14 and Rps19 genes. In homozygous T-DNA mutant lines of both Rps14 and Rps19 genes, the expression was estimated using RTPCR. Rps14 and Rps19 transcripts similar to wild type were present in homozygous mutant plants of Rps14 and Rps19 which indicated that T-DNA insertion has not affected their expression.

scholarly journals Discoordinate expression of the yeast mitochondrial ribosomal protein MRP1.

1990 ◽  
Vol 265 (13) ◽  
pp. 7449-7454
Author(s):  
H Dang ◽  
G Franklin ◽  
K Darlak ◽  
A F Spatola ◽  
S R Ellis
Keyword(s):  
Ribosomal Protein ◽  
Mitochondrial Ribosomal Protein

scholarly journals Mutations in the mitochondrial ribosomal protein MRPS22 lead to primary ovarian insufficiency

2018 ◽  
Vol 27 (11) ◽  
pp. 1913-1926 ◽  
Author(s):  
Anlu Chen ◽  
Dov Tiosano ◽  
Tulay Guran ◽  
Hagit N Baris ◽  
Yavuz Bayram ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Primary Ovarian Insufficiency ◽  
Ovarian Insufficiency ◽  
Mitochondrial Ribosomal Protein

scholarly journals Cell stemness is maintained upon concurrent expression of RB and the mitochondrial ribosomal protein S18-2

2020 ◽  
Vol 117 (27) ◽  
pp. 15673-15683
Author(s):  
Muhammad Mushtaq ◽  
Larysa Kovalevska ◽  
Suhas Darekar ◽  
Alexandra Abramsson ◽  
Henrik Zetterberg ◽  
...  
Keyword(s):  
Stem Cell ◽  
Ribosomal Protein ◽  
Severe Combined Immunodeficiency ◽  
Ectopic Expression ◽  
Embryonic Stem ◽  
Ring Finger ◽  
Histone H2a ◽  
Mitochondrial Ribosomal Protein ◽  
Primary Mouse

Stemness encompasses the capability of a cell for self-renewal and differentiation. The stem cell maintains a balance between proliferation, quiescence, and regeneration via interactions with the microenvironment. Previously, we showed that ectopic expression of the mitochondrial ribosomal protein S18-2 (MRPS18-2) led to immortalization of primary fibroblasts, accompanied by induction of an embryonic stem cell (ESC) phenotype. Moreover, we demonstrated interaction between S18-2 and the retinoblastoma-associated protein (RB) and hypothesized that the simultaneous expression of RB and S18-2 is essential for maintaining cell stemness. Here, we experimentally investigated the role of S18-2 in cell stemness and differentiation. Concurrent expression of RB and S18-2 resulted in immortalization ofRb1−/−primary mouse embryonic fibroblasts and in aggressive tumor growth in severe combined immunodeficiency mice. These cells, which express both RB and S18-2 at high levels, exhibited the potential to differentiate into various lineages in vitro, including osteogenic, chondrogenic, and adipogenic lineages. Mechanistically, S18-2 formed a multimeric protein complex with prohibitin and the ring finger protein 2 (RNF2). This molecular complex increased the monoubiquitination of histone H2ALys119, a characteristic trait of ESCs, by enhanced E3-ligase activity of RNF2. Furthermore, we found enrichment of KLF4 at theS18-2promoter region and that theS18-2expression is positively correlated withKLF4levels. Importantly, knockdown of S18-2 in zebrafish larvae led to embryonic lethality. Collectively, our findings suggest an important role for S18-2 in cell stemness and differentiation and potentially also in cancerogenesis.

Sign in / Sign up

Export Citation Format

Share Document