Isolation of an extragenic suppressor of the rna1-1 mutation in Saccharomyces cerevisiae

1998 ◽  
Vol 259 (4) ◽  
pp. 404-413 ◽  
Author(s):  
S. J. Hong ◽  
Y. S. Yi ◽  
S. S. Koh ◽  
O. K. Park ◽  
H. S. Kang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Extragenic Suppressor

The SIR1 gene of Saccharomyces cerevisiae and its role as an extragenic suppressor of several mating-defective mutants

1991 ◽  
Vol 11 (4) ◽  
pp. 2253-2262
Author(s):  
E M Stone ◽  
M J Swanson ◽  
A M Romeo ◽  
J B Hicks ◽  
R Sternglanz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Gene Product ◽  
Open Reading Frame ◽  
Histone H4 ◽  
Reading Frame ◽  
Acid Protein ◽  
Extragenic Suppressor ◽  
Mating Type Genes ◽  
Amino Acid Protein

The SIR1 gene product of Saccharomyces cerevisiae is one of several proteins involved in repressing transcription of the silent mating-type genes. Strains with mutations in the genes coding for these proteins are defective in mating due to derepression of the silent loci. We have found that overexpression of the SIR1 gene suppresses the mating defects of several of these mutants, including nat1 and ard1 mutants (the products of these two genes are responsible for N-terminal acetylation of a subset of yeast proteins), certain sir3 mutants, and a histone H4 mutant. The SIR1 gene has been sequenced and found to contain an open reading frame coding for a 678-amino-acid protein.

scholarly journals The SIR1 gene of Saccharomyces cerevisiae and its role as an extragenic suppressor of several mating-defective mutants.

1991 ◽  
Vol 11 (4) ◽  
pp. 2253-2262 ◽  
Author(s):  
E M Stone ◽  
M J Swanson ◽  
A M Romeo ◽  
J B Hicks ◽  
R Sternglanz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Gene Product ◽  
Open Reading Frame ◽  
Histone H4 ◽  
Reading Frame ◽  
Acid Protein ◽  
Extragenic Suppressor ◽  
Mating Type Genes ◽  
Amino Acid Protein

The SIR1 gene product of Saccharomyces cerevisiae is one of several proteins involved in repressing transcription of the silent mating-type genes. Strains with mutations in the genes coding for these proteins are defective in mating due to derepression of the silent loci. We have found that overexpression of the SIR1 gene suppresses the mating defects of several of these mutants, including nat1 and ard1 mutants (the products of these two genes are responsible for N-terminal acetylation of a subset of yeast proteins), certain sir3 mutants, and a histone H4 mutant. The SIR1 gene has been sequenced and found to contain an open reading frame coding for a 678-amino-acid protein.

scholarly journals Suppression of an Hsp70 Mutant Phenotype in Saccharomyces cerevisiae through Loss of Function of the Chromatin Component Sin1p/Spt2p

1998 ◽  
Vol 180 (24) ◽  
pp. 6484-6492 ◽  
Author(s):  
Bonnie K. Baxter ◽  
Elizabeth A. Craig
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Molecular Chaperones ◽  
Temperature Sensitive ◽  
Loss Of Function ◽  
Wild Type ◽  
Extragenic Suppressor ◽  
Slow Growing ◽  
Type Strains ◽  
Existing Data

ABSTRACT The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded bySSA1, SSA2, SSA3, andSSA4. Deletion of the two constitutively expressed genes,SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that an extragenic suppressor of the temperature sensitivity of ssa1 ssa2 strains, EXA1-1, is a loss-of-function mutation in SIN1/SPT2, which encodes a nonhistone component of chromatin. Loss of function of Sin1p leads to overexpression ofSSA3 in the ssa1 ssa2 mutant background, at a level which is sufficient to mediate suppression. In a strain which is wild type for SSA genes, we detected no effect of Sin1p on Ssa3p expression except under conditions of heat shock. Existing data indicate that expression of SSA3 in thessa1 ssa2 mutant background as well as in heat-shocked wild-type strains is mediated by the heat shock transcription factor HSF. Our findings suggest that it is HSF-mediated induction of SSA3 which is modulated by Sin1p. TheEXA1-1 suppressor mutation thus improves the growth ofssa1 ssa2 strains by selectively increasing HSF-mediated expression of SSA3.

Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease: a Family Study

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Study ◽  
Inflammatory Bowel

Immunreaktion auf Saccharomyces cerevisiae Antigene in Patienten mit Morbus Crohn

2007 ◽  
Vol 45 (08) ◽  
Author(s):  
S Schmechel ◽  
V Schachinger ◽  
F Seibold ◽  
C Tillack ◽  
T Ochsenkühn ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Morbus Crohn

ИММУННЫЙ СТАТУС ОРГАНИЗМА МЯСНЫХ КУР ПРИ ИСПОЛЬЗОВАНИИ ПРОБИОТИКОВ В РАЦИОНЕ

2019 ◽  
Keyword(s):  
Saccharomyces Cerevisiae

ОВЧИННИКОВ А.А., ОВЧИННИКОВА Л.Ю., КОНОВАЛОВ Д.А. Южно-Уральский государственный аграрный университет Аннотация: В опыте были изучены титры антител против основных инфекционных заболеваний птицы (инфекционный бронхит кур ИБК, болезнь Гамборо, ньюкаслская болезнь) в крови у мясных кур кросса ISA Hubbard F-15, получавших пробиотические кормовые добавки на основе Saccharomyces cerevisiae или Васillus subtilis в количестве 0,50 кг/т комбикорма. Исследования показали, что цыплята суточного возраста уже имеют врожденный иммунитет к широко распространенным инфекционным заболеваниям птицы; титры антител к ИБК и болезни Гамборо до 59-суточного возраста имели тенденцию к снижению, затем повышались и достигали максимума к 151-суточному возрасту, затем плавно снижались. У птицы, получавшей добавки пробиотиков, титр антител к ИБК за продуктивный цикл был выше на 11,2-46,1%, к болезни Гамборо - на 7,2-26,8%, ньюкаслской болезни - на 14,4-27,9% по сравнению с контрольной группой. Установлено также, что периодическое применение пробиотиков в первые 45 сут. выращивания ремонтного молодняка, в периоды разноса кур, пика продуктивности и снижения яйцекладки повышает сохранность поголовья на 0,4-8,2%. Ключевые слова: МЯСНЫЕ КУРЫ, РЕМОНТНЫЙ МОЛОДНЯК, ПРОДУКТИВНЫЙ ПЕРИОД, КОРМОВАЯ ДОБАВКА, ПРОБИОТИКИ, СОХРАННОСТЬ, ТИТРЫ АНТИТЕЛ,

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