Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestans)

1998 ◽  
Vol 259 (2) ◽  
pp. 150-160 ◽  
Author(s):  
R. C. Meyer ◽  
D. Milbourne ◽  
C. A. Hackett ◽  
J. E. Bradshaw ◽  
J. W. McNichol ◽  
...  
Keyword(s):  
Linkage Analysis ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Quantitative Resistance ◽  
Tetraploid Potato

scholarly journals Tagging Quantitative Trait Loci for Maturity-Corrected Late Blight Resistance in Tetraploid Potato with PCR-Based Candidate Gene Markers

2004 ◽  
Vol 17 (10) ◽  
pp. 1126-1138 ◽  
Author(s):  
Christina Angelika Bormann ◽  
Andreas Marcus Rickert ◽  
Rosa Angela Castillo Ruiz ◽  
Jürgen Paal ◽  
Jens Lübeck ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Late Blight ◽  
Candidate Genes ◽  
Quantitative Trait ◽  
Quantitative Resistance ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Resistance Qtl ◽  
Tetraploid Potato ◽  
Plant Maturity

Late blight caused by the oomycete Phytophthora infestans is the economically most important and destructive disease in potato cultivation. Quantitative resistance to late blight available in tetraploid cultivars is correlated with late maturity in temperate climates, which is an undesirable characteristic. A total of 30 DNA-based markers known to be linked to loci for pathogen resistance in diploid potato were selected and tested as polymerase chain reaction-based markers for linkage with quantitative trait loci (QTL) for late blight resistance and plant maturity in two half-sib families of tetraploid potatoes. Most markers originated from within or were physically closely linked to candidate genes for quantitative resistance factors. The families were repeatedly evaluated in the field for quantitative resistance to late blight and maturity. Resistance was corrected for the maturity effect. Nine of eleven different map segments tagged by the markers harbored QTL affecting maturity-corrected resistance. Interactions were found between unlinked resistance QTL, providing testable strategies for marker-assisted selection in tetraploid potato. Based on the linkage observed between QTL for resistance and plant maturity and based on the genetic interactions observed between candidate genes tagging resistance QTL, we discuss models for the molecular basis of quantitative resistance and maturity.

scholarly journals Identification and mapping of quantitative resistance to late blight (Phytophthora infestans) in Solanum habrochaites LA1777

Euphytica ◽  
2011 ◽  
Vol 179 (3) ◽  
pp. 427-438 ◽  
Author(s):  
Junming Li ◽  
Lei Liu ◽  
Yuling Bai ◽  
Richard Finkers ◽  
Feng Wang ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Quantitative Resistance ◽  
Solanum Habrochaites

scholarly journals IDENTIFICATION OF STABLE RESISTANCE TO PHYTOPHTHORA INFESTANS IN POTATO GENOTYPES EVALUATED IN FIELD EXPERIMENTS IN PERU

2007 ◽  
Vol 43 (3) ◽  
pp. 353-363 ◽  
Author(s):  
E. G. WULFF ◽  
W. PÉREZ ◽  
R. J. NELSON ◽  
M. BONIERBALE ◽  
J. A. LANDEO ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Principal Components ◽  
Field Experiments ◽  
Quantitative Resistance ◽  
Year Effect ◽  
Progress Curve ◽  
Ammi Analysis ◽  
Stability Of Resistance ◽  
Moderately Resistant

In this study, genotype by environment (G × E) interactions and phenotypic stability of resistance to Phytophthora infestans, the cause of late blight, were analysed in Peru for 13 potato genotypes, using additive main effects and multiplicative interaction (AMMI) analysis and Huehn's non-parametric test. The potato genotypes were tested in seven experiments over two years in the vicinity of Comas, Peru, an area used by the International Potato Center to screen for resistance to late blight. Results of the two analyses generally correlated and indicated that quantitative resistance to P. infestans was stable under field conditions for all the genotypes tested. The first two principal components (PCA1 and PCA2) of the AMMI analysis explained 54% of the G × E interaction sum of squares. A biplot of the two principal components demonstrated a strong year effect in the G × E interaction. By plotting yearly means of the relative area under the disease progress curve (rAUDPC), genotypes could be assigned to one of three resistance categories, resistant, moderately resistant or susceptible, but the need for a more readily applicable and truly quantitative scale for resistance was noted. Based on these studies, standards can be selected for use in screening in the area where they were tested. After further testing in other locations, a standard set drawn from these genotypes could be identified and a quantitative scale developed for evaluation of resistance under short day conditions.

QTL analysis of quantitative resistance to Phytophthora infestans (late blight) in tomato and comparisons with potato

Genome ◽  
2004 ◽  
Vol 47 (3) ◽  
pp. 475-492 ◽  
Author(s):  
Douglas J Brouwer ◽  
Elizabeth S Jones ◽  
Dina A. St Clair
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Quantitative Resistance ◽  
Interval Mapping ◽  
Lycopersicon Hirsutum ◽  
Resistance Qtls ◽  
Functional Conservation ◽  
Whole Plant ◽  
Backcross Populations ◽  
Assay Methods

Quantitative trait loci (QTLs) for resistance to Phytophthora infestans (late blight) were mapped in tomato. Reciprocal backcross populations derived from cultivated Lycopersicon esculentum × wild Lycopersicon hirsutum (BC-E, backcross to L. esculentum; BC-H, backcross to L. hirsutum) were phenotyped in three types of replicated disease assays (detached-leaflet, whole-plant, and field). Linkage maps were constructed for each BC population with RFLPs. Resistance QTLs were identified on all 12 tomato chromosomes using composite interval mapping. Six QTLs in BC-E (lb1a, lb2a, lb3, lb4, lb5b, and lb11b) and two QTLs in BC-H (lb5ab and lb6ab) were most consistently detected in replicated experiments or across assay methods. Lycopersicon hirsutum alleles conferred resistance at all QTLs except lb2a. Resistance QTLs coincided with QTLs for inoculum droplet dispersal on leaves, a trait in L. hirsutum that may contribute to resistance, and dispersal was mainly associated with leaf resistance. Some P. infestans resistance QTLs detected in tomato coincided with chromosomal locations of previously mapped R genes and QTLs for resistance to P. infestans in potato, suggesting functional conservation of resistance within the Solanaceae.Key words: late blight, tomato, Lycopersicon hirsutum, QTL mapping, disease resistance, potato.

Discerning strains of Phytophthora infestans the causative agent of potato late blight by SSCP analysis of virulence genes

2018 ◽  
Vol 34 (6) ◽  
pp. 4-11
Author(s):  
V.K. Chizhik ◽  
◽  
E.A. Sokolova ◽  
V.V. Martynov ◽  
M.A. Kuznetsova ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Causative Agent ◽  
Virulence Genes ◽  
Potato Late Blight ◽  
Sscp Analysis

Evaluation of bio-agents and neem based products against late blight disease (Phytophthora infestans) of potato

2021 ◽  
Vol 74 (1) ◽  
pp. 181-187
Author(s):  
Mehi Lal ◽  
Sorabh Chaudhary ◽  
Sanjay Rawal ◽  
Sanjeev Sharma ◽  
Manoj Kumar ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Late Blight Disease ◽  
Blight Disease

scholarly journals Identification of the Dominant Genotypes of Phytophthora infestans in Canada Using Real-Time PCR with ASO-PCR Assays

Plant Disease ◽  
2016 ◽  
Vol 100 (7) ◽  
pp. 1482-1491 ◽  
Author(s):  
Marie-Claude Gagnon ◽  
Lawrence Kawchuk ◽  
D. Mathieu Tremblay ◽  
Odile Carisse ◽  
Giovanna Danies ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Rapid Assessment ◽  
Nucleotide Polymorphisms ◽  
Blind Test ◽  
Phytophthora Spp ◽  
Allele Specific ◽  
Flanking Sequences ◽  
Pcr Assays ◽  
And Control

Phytophthora infestans, a pathogenic oomycete that is the causal agent of potato and tomato late blight, has devastating effects worldwide. The genetic composition of P. infestans populations in Canada has changed considerably over the last few years, with the appearance of several new genotypes showing different mating types and sensitivity to the fungicide metalaxyl. Genetic markers allowing for a rapid assessment of genotypes from small amounts of biological material would be beneficial for the early detection and control of this pathogen throughout Canada. Mining of the P. infestans genome revealed several regions containing single-nucleotide polymorphisms (SNP) within both nuclear genes and flanking sequences of microsatellite loci. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) assays were developed from 14 of the 50 SNP found by sequencing. Nine optimized ASO-PCR assays were validated using a blind test comprising P. infestans and other Phytophthora spp. The assays revealed diagnostic profiles unique to each of the five dominant genotypes present in Canada. The markers developed in this study can be used with environmental samples such as infected leaves, and will contribute to the genomic toolbox available to assess the genetic diversity of P. infestans at the intraspecific level. For late blight management, early warning about P. infestans genotypes present in potato and tomato fields will help growers select the most appropriate fungicides and application strategies.

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