The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site

1998 ◽  
Vol 258 (5) ◽  
pp. 538-545 ◽  
Author(s):  
B. Jürgen ◽  
T. Schweder ◽  
M. Hecker
Keyword(s):  
Bacillus Subtilis ◽  
Binding Site ◽  
Ribosome Binding Site ◽  
Ribosome Binding ◽  
The Stability

pGR71 plasmid promotor sequence temporally regulated in Bacillus subtilis

1998 ◽  
Vol 44 (12) ◽  
pp. 1186-1192
Author(s):  
Guy Daxhelet ◽  
Philippe Gilot ◽  
Etienne Nyssen ◽  
Philippe Hoet
Keyword(s):  
Bacillus Subtilis ◽  
Binding Site ◽  
Transcription Initiation ◽  
Promoter Sequence ◽  
Initiation Site ◽  
Chloramphenicol Acetyltransferase ◽  
Ribosome Binding Site ◽  
Chloramphenicol Resistance ◽  
E Coli ◽  
Ribosome Binding

pGR71, a composite of plasmids pUB110 and pBR322, replicates in Escherichia coli and in Bacillus subtilis. It carries the chloramphenicol resistance gene (cat) from Tn9, which is not transcribed in either host by lack of a promoter. The cat gene is preceded by a Shine-Dalgarno sequence functional in E. coli but not in B. subtilis. Deleted pGR71 plasmids were obtained in B. subtilis when cloning foreign viral DNA upstream of this cat sequence, as well as by BAL31 exonuclease deletions extending upstream from the cat into the pUB110 moiety. These mutant plasmids expressed chloramphenicol acetyltransferase (CAT), conferring on B. subtilis resistance to high chloramphenicol concentrations. CAT expression peaked at the early postexponential phase of B. subtilis growth. The transcription initiation site of cat, determined by primer extension, was located downstream of a putative promoter sequence within the pUB110 moiety. N-terminal amino acid sequencing showed that native CAT was produced by these mutant plasmids. The cat ribosome-binding site, functional in E. coli, was repositioned within the pUB110 moiety and had consequently an extended homology with B. subtilis 16S rRNA, explaining the production of native enzyme.Key words: chloramphenicol acetyltransferase, Bacillus subtilis, postexponential gene expression, plasmid pUB110, ribosome-binding site, transcriptional promoter.

scholarly journals A Novel Bacillus subtilis Gene,antE, Temporally Regulated and Convergent to and Overlapping dnaE

1999 ◽  
Vol 181 (1) ◽  
pp. 353-356 ◽  
Author(s):  
Lin-Fa Wang ◽  
Sung-Soo Park ◽  
Roy H. Doi
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Binding Site ◽  
Ribosome Binding Site ◽  
Small Protein ◽  
Ribosome Binding ◽  
Untranslated Sequence ◽  
Partial Homology ◽  
Subtilis Gene

ABSTRACT A Bacillus subtilis promoter, Px, that functions in a convergent manner with the sigA operon promoter P3 has been found in the sigA operon. Promoter Px is turned on at the same time as promoter P3 during early sporulation. The transcript from promoter Px codes for a small protein with partial homology to the OmpR protein from Escherichia coli and also carries an untranslated sequence at its 3′ end that is complementary to the 5′ end of the P3 transcript, which codes for the ribosome binding site ofdnaE. The gene controlled by Px has been calledantE. The expression of antE does not require ςB, ςE, or ςH. Px was transcribed in vitro by the ςA holoenzyme and is the seventh promoter to be recognized in the ςA operon. A possible role for the antE gene during early sporulation is proposed.

Small molecules targeting ribosome binding site of ricin toxin a subunit

Toxicon ◽  
2020 ◽  
Vol 177 ◽  
pp. S45
Author(s):  
Xiao-Ping Li ◽  
Nilgun E. Tumer ◽  
Jennifer Nielsen Kahn
Keyword(s):  
Small Molecules ◽  
Binding Site ◽  
Ribosome Binding Site ◽  
Toxin A ◽  
Ricin Toxin ◽  
Ribosome Binding ◽  
A Subunit
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