Functional analysis of promoter sequences of cephalosporin C biosynthesis genes from Acremonium chrysogenum: specific DNA-protein interactions and characterization of the transcription factor PACC

2001 ◽  
Vol 265 (3) ◽  
pp. 508-518 ◽  
Author(s):  
E K. Schmitt ◽  
R. Kempken ◽  
U. Kück
Keyword(s):  
Functional Analysis ◽  
Transcription Factor ◽  
Protein Interactions ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Promoter Sequences ◽  
Dna Protein Interactions

A Myb transcription factor represses conidiation and cephalosporin C production in Acremonium chrysogenum

2018 ◽  
Vol 118 ◽  
pp. 1-9 ◽  
Author(s):  
Ying Wang ◽  
Pengjie Hu ◽  
Honghua Li ◽  
Yanling Wang ◽  
Liang-kun Long ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Myb Transcription Factor ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Cephalosporin C Production

scholarly journals Functional analysis of the competence transcription factor ComK of Bacillus subtilis by characterization of truncation variants

Microbiology ◽  
2006 ◽  
Vol 152 (2) ◽  
pp. 473-483 ◽  
Author(s):  
Kim A. Susanna ◽  
Fabrizia Fusetti ◽  
Andy-Mark W. H. Thunnissen ◽  
Leendert W. Hamoen ◽  
Oscar P. Kuipers
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Dna Binding ◽  
Protein Interactions ◽  
Transcription Activation ◽  
Wild Type ◽  
Protein Protein Interactions ◽  
Protein Dna Interactions ◽  
Lower Protein

The competence transcription factor ComK is the master regulator of competence development in Bacillus subtilis. In the regulatory pathway, ComK is involved in different interactions: (i) protein–DNA interactions to stimulate transcription of ComK-dependent genes and (ii) protein–protein interactions, divided into interactions with other proteins and interactions between ComK proteins involving oligomerization. The fact that ComK displays different types of interactions suggests the presence of specific, distinct domains in the protein. This paper describes a search for functional domains, by constructing ComK truncation variants, which were tested for DNA binding, oligomerization and transcription activation. Truncations at the C-terminal end of ComK demonstrated the requirement of this part for transcription activation, but not for DNA binding. The C-terminal region is probably involved in oligomerization of ComK-dimers into tetramers. Surprisingly, a ComK truncation variant lacking 9 aa from the N-terminal end (ΔN9ComK) showed higher transcription activation than wild-type ComK, when expressed in Lactococcus lactis. However, in B. subtilis, transcription activation by ΔN9ComK was twofold lower than that by wild-type ComK, resulting from a five- to sixfold lower protein level of ComKΔN9. Thus, relatively, ΔN9ComK is more active in transcription activation than wild-type ComK. These results suggest that the presence of this N-terminal extension on ComK is a trade-off between high transcription activation and a thus far unidentified role in regulation of ComK.

scholarly journals Transcriptional regulation of the human osteopontin promoter: functional analysis and DNA-protein interactions

Oncogene ◽  
2000 ◽  
Vol 19 (50) ◽  
pp. 5801-5809 ◽  
Author(s):  
Dongyan Wang ◽  
Shunsuke Yamamoto ◽  
Naoki Hijiya ◽  
Etty N Benveniste ◽  
Candece L Gladson
Keyword(s):  
Functional Analysis ◽  
Transcriptional Regulation ◽  
Protein Interactions ◽  
Dna Protein Interactions
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