The X element, a novel LINE transposable element from Drosophila melanogaster

2001 ◽  
Vol 265 (3) ◽  
pp. 489-496 ◽  
Author(s):  
M. Tudor ◽  
A.J. Davis ◽  
M. Feldman ◽  
M. Grammatikaki ◽  
K. O'Hare
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element

scholarly journals hobo enhancer trapping mutagenesis in Drosophila reveals an insertion specificity different from P elements.

Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1063-1076 ◽  
Author(s):  
D Smith ◽  
J Wohlgemuth ◽  
B R Calvi ◽  
I Franklin ◽  
W M Gelbart
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Enhancer Trap ◽  
P Element ◽  
Present Evidence ◽  
P Elements ◽  
Element Insertion ◽  
Enhancer Trapping ◽  
Indispensable Tool

Abstract P element enhancer trapping has become an indispensable tool in the analysis of the Drosophila melanogaster genome. However, there is great variation in the mutability of loci by these elements such that some loci are relatively refractory to insertion. We have developed the hobo transposable element for use in enhancer trapping and we describe the results of a hobo enhancer trap screen. In addition, we present evidence that a hobo enhancer trap element has a pattern of insertion into the genome that is different from the distribution of P elements in the available database. Hence, hobo insertion may facilitate access to genes resistant to P element insertion.

scholarly journals Direct determination of retrotransposon transposition rates in Drosophila melanogaster

1994 ◽  
Vol 63 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Sergey V. Nuzhdin ◽  
Trudy F. C. Mackay
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Inbred Line ◽  
Copy Number ◽  
Direct Determination ◽  
Hybridization Analysis ◽  
Spontaneous Mutations ◽  
Insertion Sites

SummaryRates of transposition and excision of the Drosophila melanogaster retrotransposon elements mdg3, 297, Doc, roo and copia were estimated directly, by in situ hybridization analysis of their cytological insertion sites in 31 replicates of a highly inbred line that had accumulated spontaneous mutations for approximately 160generations. Estimated transposition rates of Doc, roo and copia were, respectively, 4·2 × 10−5, 3·1 × 10−3 and 1·3 − 10−3; no transpositions of 297 nor mdg3 were observed. Rates of transposition of copia varied significantly among sublines. Excisions were only observed for roo elements, at a rate of 9·0 × 10−6 per element per generation. Copy number averaged over these element families increased 5·9 %; therefore, in these lines the magnitude of the forces opposing transposable element multiplication were weaker than transposition rates.

An Analysis of Polygenes Affecting Wing Shape on Chromosome 2 inDrosophila melanogaster

Genetics ◽  
2001 ◽  
Vol 159 (3) ◽  
pp. 1045-1057 ◽  
Author(s):  
Kenneth Weber ◽  
Robert Eisman ◽  
Shawn Higgins ◽  
Lisa Morey ◽  
April Patty ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Genetic Effects ◽  
Wing Shape ◽  
Pleiotropic Effects ◽  
Phenotypic Variance ◽  
Chromosome 2 ◽  
Additive Effects ◽  
Chromosome Segments ◽  
Small Additive

AbstractGenetic effects on an index of wing shape on chromosome 2 of Drosophila melanogaster were mapped using isogenic recombinants with transposable element markers. At least 10 genes with small additive effects are dispersed evenly along the chromosome. Many interactions exist, with only small net effects in homozygous recombinants and little effect on phenotypic variance. Heterozygous chromosome segments show almost no dominance. Pleiotropic effects on leg shape are only minor. At first view, wing shape genes form a rather homogeneous class, but certain complexities remain unresolved.

scholarly journals Control of expression of the I factor, a LINE-like transposable element in Drosophila melanogaster.

1996 ◽  
Vol 15 (12) ◽  
pp. 3174-3181 ◽  
Author(s):  
A. Udomkit ◽  
S. Forbes ◽  
C. McLean ◽  
I. Arkhipova ◽  
D. J. Finnegan
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
I Factor ◽  
Control Of Expression

Evidence for a functional interaction between the Bari1 transposable element and the cytochrome P450 cyp12a4 gene in Drosophila melanogaster

Gene ◽  
2005 ◽  
Vol 357 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Renè Massimiliano Marsano ◽  
Ruggiero Caizzi ◽  
Roberta Moschetti ◽  
Nikolaj Junakovic
Keyword(s):  
Drosophila Melanogaster ◽  
Cytochrome P450 ◽  
Transposable Element ◽  
Functional Interaction

Molecular analysis of the P-M gonadal dysgenesis cline in eastern Australian Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 119 (4) ◽  
pp. 889-902
Author(s):  
I A Boussy ◽  
M J Healy ◽  
J G Oakeshott ◽  
M G Kidwell
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Gonadal Dysgenesis ◽  
Molecular Techniques ◽  
P Element ◽  
Latitudinal Cline ◽  
Deletion Derivative ◽  
P Elements ◽  
Regulatory Ability ◽  
Eastern Australia

Abstract The latitudinal cline in P-M gonadal dysgenesis potential in eastern Australia has been shown to comprise three regions which are, from north to south respectively, P, Q, and M, with the P-to-Q and Q-to-M transitions occurring over relatively short distances. The P element complements of 30 lines from different regions of the cline were determined by molecular techniques. The total amount of P element-hybridizing DNA was high in all lines, and it did not correlate in any obvious way with the P-M phenotypes of individual lines. The number of potentially full-sized P elements per genome was high in lines from the P regions, but variable or low among lines from the Q and M regions, and thus declined overall from north to south. A particular P element deletion-derivative, the KP element, occurred in all the tested lines. The number of KP elements was low in lines from the P region, much higher in lines from the Q region, and highest among lines from the M region, thus forming a cline reciprocal to that of the full-sized P elements. Another transposable element, hobo, which has been described as causing dysgenic traits similar to those of P-M hybrid dysgenesis, was shown to be present in all lines and to vary among them in number, but not in any latitudinal pattern. The P-M cline in gonadal dysgenesis potential can be inferred to be based on underlying clinal patterns of genomic P element complements. P activity of a line was positively correlated with the number of full-sized P elements in the line, and negatively correlated with the number of KP elements. Among Q and M lines, regulatory ability was not correlated with numbers of KP elements.

Transposition of the vertebrate Tol2 transposable element in Drosophila melanogaster

Gene ◽  
2008 ◽  
Vol 425 (1-2) ◽  
pp. 64-68 ◽  
Author(s):  
Akihiro Urasaki ◽  
Taro Mito ◽  
Sumihare Noji ◽  
Ryu Ueda ◽  
Koichi Kawakami
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element

scholarly journals The Drosophila melanogaster gypsy transposable element encodes putative gene products homologous to retroviral proteins.

1986 ◽  
Vol 6 (4) ◽  
pp. 1129-1134 ◽  
Author(s):  
R L Marlor ◽  
S M Parkhurst ◽  
V G Corces
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Transcription Initiation ◽  
Primer Binding Site ◽  
Long Terminal Repeats ◽  
Gypsy Element ◽  
Amino Acid Homology ◽  
Putative Gene ◽  
Putative Protein ◽  
Terminal Repeats

We determined the complete nucleotide sequence of the gypsy element present at the forked locus of Drosophila melanogaster in the f1 allele. The gypsy element shares more homology with vertebrate retroviruses than with the copia element of D. melanogaster or the Ty element of Saccharomyces cerevisiae, both in overall organization and at the DNA sequence level. This transposable element is 7,469 base pairs long and encodes three putative protein products. The long terminal repeats are 482 nucleotides long and contain transcription initiation and termination signals; sequences homologous to the polypurine tract and tRNA primer binding site of retroviruses are located adjacent to the long terminal repeats. The central region of the element contains three different open reading frames. The second one encodes a putative protein which shows extensive amino acid homology to retroviral proteins, including gag-specific protease, reverse transcriptase, and DNA endonuclease.

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