Mutations in the SAM domain of STE50 differentially influence the MAPK-mediated pathways for mating, filamentous growth and osmotolerance in Saccharomyces cerevisiae

2000 ◽  
Vol 265 (1) ◽  
pp. 102-117 ◽  
Author(s):  
G. Jansen ◽  
F. Bühring ◽  
C. P. Hollenberg ◽  
M. Ramezani Rad
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Filamentous Growth ◽  
Sam Domain

scholarly journals Filamentous growth of the budding yeast Saccharomyces cerevisiae induced by overexpression of the WHI2 gene

Microbiology ◽  
1997 ◽  
Vol 143 (6) ◽  
pp. 1867-1876 ◽  
Author(s):  
P. A. Radcliffe ◽  
K. M. Binley ◽  
J. Trevethick ◽  
M. Hall ◽  
P. E. Sudbery
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Budding Yeast ◽  
Filamentous Growth ◽  
Yeast Saccharomyces Cerevisiae

Characterization of industrial strains of Saccharomyces cerevisiae exhibiting filamentous growth induced by alcohols and nutrient deprivation

2006 ◽  
Vol 23 (5) ◽  
pp. 697-704 ◽  
Author(s):  
Paula Cristina da Silva ◽  
Jorge Horii ◽  
Viviane Santos Miranda ◽  
Heloísa Gallera Brunetto ◽  
Sandra Regina Ceccato-Antonini
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Filamentous Growth ◽  
Nutrient Deprivation ◽  
Industrial Strains

GPR1 Regulates Filamentous Growth through FLO11 in Yeast Saccharomyces cerevisiae

2000 ◽  
Vol 267 (1) ◽  
pp. 164-168 ◽  
Author(s):  
Hisanori Tamaki ◽  
Takuya Miwa ◽  
Makiko Shinozaki ◽  
Megumi Saito ◽  
Cheol-Won Yun ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Filamentous Growth ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals Cyclic AMP-Dependent Protein Kinase Regulates Pseudohyphal Differentiation in Saccharomyces cerevisiae

1999 ◽  
Vol 19 (7) ◽  
pp. 4874-4887 ◽  
Author(s):  
Xuewen Pan ◽  
Joseph Heitman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Kinase ◽  
Map Kinase ◽  
Filamentous Growth ◽  
Dependent Protein Kinase ◽  
Catalytic Subunits ◽  
Map Kinase Cascade ◽  
Kinase Cascade ◽  
Dependent Protein ◽  
Pseudohyphal Differentiation

ABSTRACT In response to nitrogen starvation, diploid cells of the yeastSaccharomyces cerevisiae differentiate to a filamentous growth form known as pseudohyphal differentiation. Filamentous growth is regulated by elements of the pheromone mitogen-activated protein (MAP) kinase cascade and a second signaling cascade involving the receptor Gpr1, the Gα protein Gpa2, Ras2, and cyclic AMP (cAMP). We show here that the Gpr1-Gpa2-cAMP pathway signals via the cAMP-dependent protein kinase, protein kinase A (PKA), to regulate pseudohyphal differentiation. Activation of PKA by mutation of the regulatory subunit Bcy1 enhances filamentous growth. Mutation and overexpression of the PKA catalytic subunits reveal that the Tpk2 catalytic subunit activates filamentous growth, whereas the Tpk1 and Tpk3 catalytic subunits inhibit filamentous growth. The PKA pathway regulates unipolar budding and agar invasion, whereas the MAP kinase cascade regulates cell elongation and invasion. Epistasis analysis supports a model in which PKA functions downstream of the Gpr1 receptor and the Gpa2 and Ras2 G proteins. Activation of filamentous growth by PKA does not require the transcription factors Ste12 and Tec1 of the MAP kinase cascade, Phd1, or the PKA targets Msn2 and Msn4. PKA signals pseudohyphal growth, in part, by regulating Flo8-dependent expression of the cell surface flocculin Flo11. In summary, the cAMP-dependent protein kinase plays an intimate positive and negative role in regulating filamentous growth, and these findings may provide insight into the roles of PKA in mating, morphogenesis, and virulence in other yeasts and pathogenic fungi.

scholarly journals The Glc7p-Interacting Protein Bud14p Attenuates Polarized Growth, Pheromone Response, and Filamentous Growth in Saccharomyces cerevisiae

2002 ◽  
Vol 1 (6) ◽  
pp. 884-894 ◽  
Author(s):  
Paul J. Cullen ◽  
George F. Sprague
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fluorescent Protein ◽  
Genetic Selection ◽  
Positive Function ◽  
Filamentous Growth ◽  
Polarized Growth ◽  
Protein Fusion ◽  
Pheromone Response ◽  
Interacting Protein ◽  
Pheromone Response Pathway

ABSTRACT A genetic selection in Saccharomyces cerevisiae for mutants that stimulate the mating pathway uncovered a mutant that had a hyperactive pheromone response pathway and also had hyperpolarized growth. Cloning and segregation analysis demonstrated that BUD14 was the affected gene. Disruption of BUD14 in wild-type cells caused mild stimulation of pheromone response pathway reporters, an increase in sensitivity to mating factor, and a hyperelongated shmoo morphology. The bud14 mutant also had hyperfilamentous growth. Consistent with a role in the control of cell polarity, a Bud14p-green fluorescent protein fusion was localized to sites of polarized growth in the cell. Bud14p shared morphogenetic functions with the Ste20p and Bni1p proteins as well as with the type 1 phosphatase Glc7p. The genetic interactions between BUD14 and GLC7 suggested a role for Glc7p in filamentous growth, and Glc7p was found to have a positive function in filamentous growth in yeast.

scholarly journals Divergent Roles for cAMP–PKA Signaling in the Regulation of Filamentous Growth in Saccharomyces cerevisiae and Saccharomyces bayanus

2018 ◽  
Author(s):  
Ömur Kayikci ◽  
Paul M. Magwene
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Response ◽  
Critical Role ◽  
Filamentous Growth ◽  
Comparative Investigation ◽  
Effector Proteins ◽  
Evolutionary Divergence ◽  
Downstream Targets ◽  
Network Function ◽  
Saccharomyces Bayanus

ABSTRACTThe cyclic AMP – Protein Kinase A (cAMP–PKA) pathway is an evolutionarily conserved eukaryotic signaling network that is essential for growth and development. In the fungi, cAMP–PKA signaling plays a critical role in regulating cellular physiology and morphological switches in response to nutrient availability. We undertook a comparative investigation of the role that cAMP-PKA signaling plays in the regulation of filamentous growth in two closely related budding yeast species, Saccharomyces cerevisiae and Saccharomyces bayanus. Using chemical and genetic perturbations of this pathway and its downstream targets we discovered divergent roles for cAMP-PKA signaling in the regulation of filamentous growth. While cAMP-PKA signaling is required for the filamentous growth response in both species, increasing or decreasing the activity of this pathway leads to drastically different phenotypic outcomes. In S. cerevisiae, cAMP-PKA inhibition ameliorates the filamentous growth response while hyper-activation of the pathway leads to increased filamentous growth; the same perturbations in S. bayanus result in the obverse. Divergence in the regulation of filamentous growth between S. cerevisiae and S. bayanus extends to downstream targets of PKA, including several kinases, transcription factors, and effector proteins. Our findings highlight the potential for significant evolutionary divergence in gene network function, even when the constituent parts of such networks are well conserved.

scholarly journals Clarifying intercellular signalling in yeast: Saccharomyces cerevisiae does not undergo a quorum sensing-dependent switch to filamentous growth

2021 ◽  
Author(s):  
Michela Pia Winters ◽  
Violetta Aru ◽  
Kate Howell ◽  
Nils Arneborg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Quorum Sensing ◽  
Cell Density ◽  
Nitrogen Deficiency ◽  
Filamentous Growth ◽  
Metabolite Concentration ◽  
Cell Densities ◽  
Local Cell ◽  
Low Nitrogen ◽  
Nitrogen Conditions

Saccharomyces cerevisiae can alter its morphology to a filamentous form associated with unipolar budding in response to environmental stressors. Induction of filamentous growth is suggested under nitrogen deficiency in response to alcoholic signalling molecules through a quorum sensing mechanism. To investigate this claim, we analysed the budding pattern of S. cerevisiae cells over time under low nitrogen while concurrently measuring cell density and extracellular metabolite concentration. We found that the proportion of cells displaying unipolar budding increased between local cell densities of 4.8x106 and 5.3x107 cells/ml within 10 to 20 hours of growth. However, the observed increase in unipolar budding could not be reproduced when cells were prepared at the critical cell density and in conditioned media. Removing the nutrient restriction by growth in high nitrogen conditions also resulted in an increase in unipolar budding between local cell densities of 5.2x106 and 8.2x107 cells/ml within 10 to 20 hours of growth, but there were differences in metabolite concentration compared to the low nitrogen conditions. This suggests that neither cell density, metabolite concentration, nor nitrogen deficiency were necessary or sufficient to increase the proportion of unipolar budding cells. It is therefore unlikely that quorum sensing is the mechanism controlling the switch to filamentous growth in S. cerevisiae. Only a high concentration of the putative signalling molecule, 2-phenylethanol resulted in an increase in unipolar budding, but this concentration was not physiologically relevant. We suggest that the compound 2-phenylethanol acts through a toxicity mechanism, rather than quorum sensing, to induce filamentous growth.

Sign in / Sign up

Export Citation Format

Share Document