Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii

2001 ◽  
Vol 264 (6) ◽  
pp. 790-795 ◽  
Author(s):  
D. R. Stevens ◽  
A. Atteia ◽  
L.-G. Franzén ◽  
S. Purton
Keyword(s):  
Ribosomal Protein ◽  
Chlamydomonas Reinhardtii ◽  
Novel Mutations ◽  
Cycloheximide Resistance

scholarly journals A Gcn4p Homolog Is Essential for the Induction of a Ribosomal Protein L41 Variant Responsible for Cycloheximide Resistance in the YeastCandida maltosa

2004 ◽  
Vol 279 (22) ◽  
pp. 23030-23037 ◽  
Author(s):  
Hiroaki Takaku ◽  
Eishun Mutoh ◽  
Yoshiyuki Sagehashi ◽  
Ryouichi Fukuda ◽  
Hiroyuki Horiuchi ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Cycloheximide Resistance

scholarly journals Development of Antibiotic-Overproducing Strains by Site-Directed Mutagenesis of the rpsL Gene in Streptomyces lividans

2003 ◽  
Vol 69 (7) ◽  
pp. 4256-4259 ◽  
Author(s):  
Yoshiko Okamoto-Hosoya ◽  
Susumu Okamoto ◽  
Kozo Ochi
Keyword(s):  
Ribosomal Protein ◽  
Antibiotic Production ◽  
Strain Improvement ◽  
Streptomyces Lividans ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Novel Mutations ◽  
Conserved Regions ◽  
Silent Genes ◽  
Ribosomal Protein S12

ABSTRACT Certain rpsL (which encodes the ribosomal protein S12) mutations that confer resistance to streptomycin markedly activate the production of antibiotics in Streptomyces spp. These rpsL mutations are known to be located in the two conserved regions within the S12 protein. To understand the roles of these two regions in the activation of silent genes, we used site-directed mutagenesis to generate eight novel mutations in addition to an already known (K88E) mutation that is capable of activating antibiotic production in Streptomyces lividans. Of these mutants, two (L90K and R94G) activated antibiotic production much more than the K88E mutant. Neither the L90K nor the R94G mutation conferred an increase in the level of resistance to streptomycin and paromomycin. Our results demonstrate the efficacy of the site-directed mutagenesis technique for strain improvement.

scholarly journals Natural cycloheximide resistance in yeast. The role of ribosomal protein L41

1993 ◽  
Vol 213 (2) ◽  
pp. 841-848 ◽  
Author(s):  
Pierre DEHOUX ◽  
Julian DAVIES ◽  
Michael CANNON
Keyword(s):  
Ribosomal Protein ◽  
Cycloheximide Resistance

scholarly journals Structural and free energy landscape of novel mutations in ribosomal protein S1 (rpsA) associated with pyrazinamide resistance

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Muhammad Tahir Khan ◽  
Abbas Khan ◽  
Ashfaq Ur Rehman ◽  
Yanjie Wang ◽  
Khalid Akhtar ◽  
...  
Keyword(s):  
Free Energy ◽  
Ribosomal Protein ◽  
Energy Landscape ◽  
Free Energy Landscape ◽  
Novel Mutations ◽  
Ribosomal Protein S1
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