Mapping the genome of Eimeria tenella  : use of a yeast artificial chromosome library as the basis for a physical map

1997 ◽  
Vol 84 (1) ◽  
pp. 13-16 ◽  
Author(s):  
B. Hoogendoorn ◽  
A. A. Toye ◽  
N. Bumstead ◽  
M. W. Shirley
Keyword(s):  
Yeast Artificial Chromosome ◽  
Physical Map ◽  
Artificial Chromosome ◽  
Eimeria Tenella ◽  
Yeast Artificial Chromosome Library

A physical map with yeast artificial chromosome (YAC) clones covering 63% of the 12 rice chromosomes

Genome ◽  
2001 ◽  
Vol 44 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Shoko Saji ◽  
Yosuke Umehara ◽  
Baltazar A Antonio ◽  
Hiroko Yamane ◽  
Hiroshi Tanoue ◽  
...  
Keyword(s):  
Genetic Map ◽  
Dna Markers ◽  
Yeast Artificial Chromosome ◽  
Physical Map ◽  
Genome Structure ◽  
Rice Genome ◽  
Artificial Chromosome ◽  
Physical Length ◽  
Rice Chromosomes ◽  
Yac Contig

A new YAC (yeast artificial chromosome) physical map of the 12 rice chromosomes was constructed utilizing the latest molecular linkage map. The 1439 DNA markers on the rice genetic map selected a total of 1892 YACs from a YAC library. A total of 675 distinct YACs were assigned to specific chromosomal locations. In all chromosomes, 297 YAC contigs and 142 YAC islands were formed. The total physical length of these contigs and islands was estimated to 270 Mb which corresponds to approximately 63% of the entire rice genome (430 Mb). Because the physical length of each YAC contig has been measured, we could then estimate the physical distance between genetic markers more precisely than previously. In the course of constructing the new physical map, the DNA markers mapped at 0.0-cM intervals were ordered accurately and the presence of potentially duplicated regions among the chromosomes was detected. The physical map combined with the genetic map will form the basis for elucidation of the rice genome structure, map-based cloning of agronomically important genes, and genome sequencing.Key words: physical mapping, YAC contig, rice genome, rice chromosomes.

scholarly journals The CMT2D Locus: Refined Genetic Position and Construction of a Bacterial Clone-Based Physical Map

1999 ◽  
Vol 9 (6) ◽  
pp. 568-574 ◽  
Author(s):  
Rachel E. Ellsworth ◽  
Victor Ionasescu ◽  
Charles Searby ◽  
Val C. Sheffield ◽  
Valerie V. Braden ◽  
...  
Keyword(s):  
Linkage Analysis ◽  
Yeast Artificial Chromosome ◽  
Sequence Data ◽  
Physical Map ◽  
Muscular Atrophy ◽  
Large Family ◽  
Artificial Chromosome ◽  
Single Chromosome ◽  
Mixed Features ◽  
Bacterial Clone

Charcot-Marie-Tooth (CMT) disease is a progressive neuropathy of the peripheral nervous system, typically characterized by muscle weakness of the distal limbs. CMT is noted for its genetic heterogeneity, with four distinct loci already identified for the axonal form of the disease (CMT2). In 1996, linkage analysis of a single large family revealed the presence of a CMT2 locus on chromosome 7p14 (designatedCMT2D). Additional families have been linked subsequently to the same genomic region, including one with distal spinal muscular atrophy (dSMA) and one with mixed features of dSMA and CMT2; symptoms in both of these latter families closely resemble those seen in the original CMT2D family. There is thus a distinct possibility that CMT2 and dSMA encountered in these families reflect allelic heterogeneity at a single chromosome 7 locus. In the study reported here, we have performed more detailed linkage analysis of the original CMT2D family based on new knowledge of the physical locations of various genetic markers. The region containing the CMT2D gene, as defined by the original family, overlaps with those defined by at least two other families with CMT2 and/or dSMA symptoms. Both yeast artificial chromosome (YAC) and bacterial clone-based [bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC)] contig maps spanning ∼3.4 Mb have been assembled across the combinedCMT2D critical region, with the latter providing suitable clones for systematic sequencing of the interval. Preliminary analyses have already revealed at least 28 candidate genes and expressed-sequence tags (ESTs). The mapping information reported here in conjunction with the evolving sequence data should expedite the identification of the CMT2D/dSMA gene or genes.

Construction of a large-insert yeast artificial chromosome library of the rat genome

1997 ◽  
Vol 8 (4) ◽  
pp. 284-284 ◽  
Author(s):  
M. L. Haldi ◽  
P. Lim ◽  
K. Kaphingst ◽  
U. Akella ◽  
J. Whang ◽  
...  
Keyword(s):  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Yeast Artificial Chromosome Library ◽  
Rat Genome

A 2.8 megabase YAC contig spanning D8S339, which is tightly linked to the Werner syndrome locus

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 77-83
Author(s):  
R. Bruskiewich ◽  
M. Schertzer ◽  
S. Wood
Keyword(s):  
Human Chromosome ◽  
Genetic Marker ◽  
Yeast Artificial Chromosome ◽  
Werner Syndrome ◽  
Physical Map ◽  
Artificial Chromosome ◽  
Sequence Tagged Sites ◽  
Yeast Artificial Chromosome Contig ◽  
Yac Contig ◽  
Chromosome 8P

A number of gene loci, including the locus for Werner syndrome (WRN), map to proximal human chromosome 8p near the genetic marker D8S339. In this report, we present a long range physical map of an approximately 2.8 megabase yeast artificial chromosome contig centred on D8S339. In this map, we localize the WRN-linked polymorphic sequence-tagged sites (STS) D8S339 and D8S1055, as well as a novel polymorphic STS, D8S2297. We also refine the positions of three known gene loci, GTF2E2, GSR, and PPP2CB, relative to the location of WRN within the map.Key words: WRN, GTF2E2, GSR, PPP2CB, physical map, human chromosome 8p.

Genetic and contig map of a 2200-kb region encompassing 5.5 cM on chromosome 1 of Arabidopsis thaliana

Genome ◽  
1996 ◽  
Vol 39 (6) ◽  
pp. 1086-1092 ◽  
Author(s):  
Christian S. Hardtke ◽  
Thomas Berleth
Keyword(s):  
Arabidopsis Thaliana ◽  
Positional Cloning ◽  
Yeast Artificial Chromosome ◽  
Physical Map ◽  
Primary Root ◽  
Artificial Chromosome ◽  
Chromosome 1 ◽  
Rflp Markers ◽  
Caps Markers ◽  
Yac Contig

In the course of the isolation of the MONOPTEROS (MP) gene, required for primary root formation in Arabidopsis thaliana, a yeast artificial chromosome (YAC) contig encompassing approximately 2200 kilobases corresponding to 5.5 cM on the top arm of chromosome 1 was established. Forty-six YAC clones were characterized and 12 new restriction fragment length polymorphism (RFLP) markers are presented. Three new codominant amplified polymorphic sequence (CAPS) markers were generated that enabled high resolution genetic mapping and correlation of physical and genetic distances along the contig. The map contributes to the completion of a physical map of the Arabidopsis genome and should facilitate positional cloning of other genes in the region as well as studies on genome organization. We also present another set of 11 physically linked probes, as well as mapping data for additional RFLP markers within a broader interval of 10.4 cM. Key words : Arabidopsis, CAPS markers, MONOPTEROS gene, physical map, RFLP markers, YAC contig.

A 6-Mb Yeast Artificial Chromosome Contig and Long-Range Physical Map Encompassing the Region on Chromosome 12q15 Frequently Rearranged in a Variety of Benign Solid Tumors

Genomics ◽  
1995 ◽  
Vol 29 (3) ◽  
pp. 665-678 ◽  
Author(s):  
ERIC F.P.M. SCHOENMAKERS ◽  
JAN M.W. GEURTS ◽  
PATRICK F.J. KOOLS ◽  
RAF MOLS ◽  
CHRISTEL HUYSMANS ◽  
...  
Keyword(s):  
Solid Tumors ◽  
Long Range ◽  
Yeast Artificial Chromosome ◽  
Physical Map ◽  
Artificial Chromosome ◽  
Yeast Artificial Chromosome Contig

A comprehensive large-insert yeast artificial chromosome library for physical mapping of the mouse genome

1996 ◽  
Vol 7 (10) ◽  
pp. 767-769 ◽  
Author(s):  
M. L. Haldi ◽  
C. Strickland ◽  
P. Lim ◽  
V. VanBerkel ◽  
X. -N. Chen ◽  
...  
Keyword(s):  
Physical Mapping ◽  
Mouse Genome ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Yeast Artificial Chromosome Library
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