Effects of temperature and white sucker ( Catostomus commersoni ) serum supplement on the in vitro multiplication of Cryptobia catostomi in cell-free culture medium

1996 ◽  
Vol 82 (3) ◽  
pp. 276-278 ◽  
Author(s):  
S. Li ◽  
P. T. K. Woo
Keyword(s):  
Culture Medium ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
Serum Supplement ◽  
In Vitro Multiplication ◽  
Effects Of Temperature

In vitro culture and multiplication of Cryptobia catostomi and experimental infection of white sucker (Catostomus commersoni)

1992 ◽  
Vol 70 (2) ◽  
pp. 201-204 ◽  
Author(s):  
Philip T. Thomas ◽  
Patrick T. K. Woo
Keyword(s):  
Experimental Infection ◽  
Infected Fish ◽  
Nutritional Requirements ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
Minimum Essential Medium ◽  
Large Numbers ◽  
Division Process ◽  
Foetal Bovine Serum

Cryptobia catostomi, a parasitic haemoflagellate of the white sucker (Catostomus commersoni), was cultured in minimum essential medium (MEM) supplemented with Hanks' salts, L-glutamine, and 25% foetal bovine serum (MEM-plus). Parasite numbers were significantly higher in MEM-plus cultures supplemented with white sucker plasma than in unsupplemented cultures. This procedure is useful when large numbers of the parasite are required, e.g., for studies on their nutritional requirements, metabolism, or antigenic nature. Cultures could not be maintained at 10 °C beyond the fourth subculture; this was about 11 months after the primary culture was started. The division process in culture was similar to that reported in fish. The culture forms were infective to white suckers. Parasitaemias in white suckers infected with blood forms increased from 2 to 5 weeks postinfection and stayed relatively constant thereafter. Neither anorexia nor anaemia was evident in infected fish, confirming the nonpathogenicity of C. catostomi to white suckers.

scholarly journals LOCUST MEDIAL NEUROSECRETORY CELLS IN VITRO: MORPHOLOGY, ELECTROPHYSIOLOGICAL PROPERTIES AND EFFECTS OF TEMPERATURE

1993 ◽  
Vol 183 (1) ◽  
pp. 323-339
Author(s):  
W. Rossler ◽  
U. Bickmeyer
Keyword(s):  
Culture Medium ◽  
Locusta Migratoria ◽  
Ionic Currents ◽  
Neurosecretory Cells ◽  
Patch Clamp Technique ◽  
Pars Intercerebralis ◽  
Serum Free ◽  
Electrophysiological Properties ◽  
Effects Of Temperature

The medial neurosecretory cells of the pars intercerebralis in the protocerebrum of larval and adult locusts (Locusta migratoria) were cultured in a chemically defined serum-free culture medium. The morphology of the cells was investigated by light microscopy and the electrophysiological properties were studied using the patch-clamp technique in the whole-cell configuration. The dissociated neurosecretory cells grew new processes under these conditions and were maintained in culture for up to 2 months. The percentage of cells showing outgrowth was significantly higher in third-instar larvae than in instars 4 and 5 and adults. A primary axonal stump promoted a unipolar cell morphology; in other cases, most neurosecretory cells became multipolar. The presence of glial cells in undissociated groups of neurosecretory cells improved outgrowth and the formation of neurite bundles. A considerable number of the recorded cells showed spiking activity in response to depolarization. The influences of temperature on spike frequency, duration and amplitude as well as on membrane potential and ionic currents were investigated. The results suggest that temperature may directly affect the function of neurosecretory cells.

scholarly journals PENGARUH THIDIAZURON DAN HIDROLISAT KASEIN TERHADAP MULTIPLIKASI TUNAS SATOIMO (Colocasia esculenta (L.) Schott var antiquorum ) SECARA IN VITRO

2017 ◽  
Vol 4 (2) ◽  
pp. 17
Author(s):  
. Karyanti ◽  
Minda Kartini
Keyword(s):  
Culture Medium ◽  
Casein Hydrolysate ◽  
Shoot Multiplication ◽  
Colocasia Esculenta ◽  
Optimal Concentration ◽  
Plant Propagation ◽  
In Vitro Multiplication ◽  
Plant Seeds ◽  
Optimal Medium

Effect of Thidiazuron and Casein Hydrolysate on In Vitro Shoot Multiplication of Satoimo (Colocasia esculenta (L.) Schott var antiquorum)ABTRACTSatoimo (Colocasia esculenta (L.) Schott var antiquorum) is an alternative substitute of rice which has a big potential to be developed in Indonesia as an export commodity to Japan. Satoimo production needs to be increased to meet the demands for of the plant seeds. Plant propagation can be done using optimal media to stimulate the formation of shoots through, amongst others, the addition of thidiazuron (TDZ) and casein hydrolysate into the culture medium. This study aimed to determine the optimal concentration of TDZ and casein hydrolysate for in vitro multiplication of satoimo shoots. This research used RAL method with 2 factorials, namely the addition of TDZ at 0; 0.2; 0.6 mg/L concentrations, and of casein hydrolysate at 0; 150; 300; 450 mg/L concentrations. The results showed that the use of 0.6 mg/L TDZ and 150 mg/L casein hydrolysate resulted in the highest number of shoots, with the shoot average number of 6.9 per explant.Keywords: Casein hydrolysate, optimal medium, Satoimo, shoot multiplication, TDZ  ABTRAKSatoimo (Colocasia esculenta (L.) Schott var antiquorum) merupakan salah satu bahan alternatif pengganti beras yang memiliki peluang besar untuk dikembangkan di Indonesia, salah satunya sebagai komoditas ekspor ke negara Jepang. Produksi satoimo perlu ditingkatkan untuk memenuhi kebutuhan bibit tanaman tersebut. Perbanyakan tanaman dapat dilakukan menggunakan media yang optimal untuk merangsang pembentukan tunas, salah satunya dengan penambahan thidiazuron (TDZ) dan hidrolisat kasein pada media tanam. Penelitian ini bertujuan untuk mengetahui konsentrasi TDZ dan hidrolisat kasein yang optimal untuk perbanyakan tunas satoimo secara in vitro. Penelitian ini menggunakan metode RAL dengan 2 faktorial yaitu konsentrasi TDZ yang terdiri dari 0; 0,2; 0,6 mg/L dan konsentrasi hidrolisat kasein yang terdiri dari 0; 150; 300; 450 mg/L. Hasil penelitian menunjukkan bahwa pemberian TDZ 0,6 mg/L dan hidrolisat kasein 150 mg/L menghasilkan jumlah tunas tertinggi, dengan rata-rata tunas yang terbentuk 6,9 per eksplan.Kata kunci: Hidrolisat kasein, multiplikasi tunas, optimasi media, Satoimo, TDZ 

scholarly journals Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

2018 ◽  
Vol 48 (9) ◽  
Author(s):  
Natália Helena Gavilan ◽  
Fernanda Cardoso Furlan ◽  
Alex Zichner Zorz ◽  
Leandro Silva de Oliveira ◽  
Wellington Ferreira Campos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Culture Medium ◽  
Genetic Erosion ◽  
Chemical Agent ◽  
Shoot Induction ◽  
Popular Medicine ◽  
In Vitro Multiplication ◽  
Apical Buds ◽  
Chemical Sterilization

ABSTRACT: Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.

Effect of serum on the in vitro maturation of canine oocytes

1999 ◽  
Vol 11 (8) ◽  
pp. 387 ◽  
Author(s):  
Takeshige Otoi ◽  
Maya Fujii ◽  
Aya Ooka ◽  
Masaki Tanaka ◽  
Tatsuyuki Suzuki
Keyword(s):  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
In Vitro Maturation ◽  
Culture Medium ◽  
The Other ◽  
Serum Supplement ◽  
Meiotic Competence ◽  
Positive Effect ◽  
Serum Type

The meiotic competence of canine oocytes cultured for 72 h in medium supplemented with three different concentrations (5, 10 and 20%) of anoestrous, oestrous or metoestrous bitch serum, or with 0.3% bovine serum albumin (BSA), was examined. The oestrous serum supplement had a positive effect on the resumption of meiosis, compared with the other supplements (P<0.05). The number of oocytes that reached metaphase I (MI) and metaphase II (MII) was significantly higher (P<0.05) with the oestrous serum supplement than with the anoestrous serum supplement. There were no significant differences among the three different concentrations in each serum type with respect to the proportion of oocytes that completed meiosis (MI to MII). The number of oocytes that resumed meiosis in the 10% oestrous serum supplement was significantly higher (P<0.05) than those of each concentration of the anoestrous and metoestrous serum supplements, and of the 0.3% BSA supplement. Moreover, a higher number of oocytes reached MII in the presence of the 10% oestrous serum supplement than with the 10% anoestrous serum supplement. These results suggest that supplementation of the culture medium with 10% oestrous serum is the optimal treatment for in vitro maturation of canine oocytes.

scholarly journals Effect of different carbon sources on the in vitro multiplication of Annona sp.

2011 ◽  
Vol 35 (3) ◽  
pp. 487-493 ◽  
Author(s):  
José Raniere Ferreira de Santana ◽  
Renato Paiva ◽  
Ana Valéria de Souza ◽  
Lenaldo Muniz de Oliveira
Keyword(s):  
Culture Medium ◽  
Carbon Sources ◽  
Dry Weight ◽  
Nodal Segments ◽  
Seedling Production ◽  
In Vitro Multiplication ◽  
Bud Induction ◽  
Significant Difference ◽  
Annona Glabra

The Annonaceae family comprises approximately 2.300 species, some with significant commercial value. Although commercial plantations have suffered due to problems related to seedling production. As micropropagation is a viable technique for seedling production, the present work evaluated the effects of different carbon sources on in vitro bud induction in five Annonaceae species. Nodal segments obtained from plants of the Annona glabra, A. cauliflora, A. coriacea, A. bahiensis and Rollinia silvatica species were inoculated into solid WPM culture medium with 8.87 μM BAP, 0.86 mM of benomyl, and 87.64 mM of the following carbon sources: glucose, sucrose, fructose, galactose, sorbitol and maltose. We evaluated the buds number, the length and weight of the largest bud, the number of expanded leaves per bud, the length of the largest leaf and the dry matter of the buds. No significant difference was observed among the different carbon sources used in terms of the number of produced buds; however, the length of the largest bud, the number of expanded leaves, the length of the largest leaf, and dry weight of the buds presented significant difference according to the studied speciesas well as the carbon sources used, with the lowest value being obtained with sorbitol. The results obtained here indicated that, except for sorbitol, any of the carbohydrates tested could be used in the in vitro multiplication protocols for A. bahiensis, A. cauliflora, A. coriacea, A. glabra and R. silvatica.

scholarly journals Balanço hormonal auxina/citocinica para multiplicação in vitro de genótipos de gérbera

2021 ◽  
Vol 4 (1) ◽  
pp. 119-134
Author(s):  
Tarcisio Rangel do Couto ◽  
◽  
João Sebastião de Paula Araujo ◽  
João Paulo de Lima Aguilar ◽  
◽  
...  
Keyword(s):  
Culture Medium ◽  
Average Length ◽  
Ornamental Plants ◽  
World Market ◽  
Cut Flower ◽  
In Vitro Multiplication ◽  
Great Demand ◽  
Planting Material ◽  
The World

Gerbera is used as a cut flower and has gained popularity as ornamental flower and great demand in the world market for ornamental plants. Micropropagation is used to meet the demand for commercial planting material. The objective was to evaluate the BAP and ANA phytoregulators effect gerberas in vitro multiplication. The explants were inoculated in MS culture medium containing different concentrations of BAP (0.0; 2.22; 4.44; 8.88 and 17.76 µmol L-1) and ANA (0.0; 1.34; 2.68 and 5.36 µmol L-1). After eight weeks, the number of shoots formed in each explant and the average length of the shoots were evaluated. Was possible to establish and recommend an ideal concentration of BAP and ANA for each gerbera genotype.

scholarly journals Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

2016 ◽  
Vol 37 (5) ◽  
pp. 2901
Author(s):  
Daniele Brandstetter Rodrigues ◽  
Michele Carla Nadal ◽  
Samila Silva Camargo ◽  
Adriane Marinho de Assis ◽  
Márcia Wullf Schuch ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Aerial Part ◽  
Culture Medium ◽  
Coconut Fiber ◽  
Indole Butyric Acid ◽  
In Vitro Multiplication ◽  
Survival Percentage ◽  
Number Of Leaves ◽  
Points Of View

An adequate concentration of growth regulators as well as the replacement of agar by an alternative medium may be promising from practical and financial points of view to produce orchid plants by micropropagation. The objective of this work was to evaluate different concentrations of growth regulator and alternative substrates for agar replacement in culture medium for in vitro multiplication and rooting of Oncidium baueri. In the explant multiplication phase, two experimental factors were evaluated- various concentrations of 6-benzylaminopurine (BAP) (0.0, 1.0, 2.0, and 3.0 mg L-1) and substrates (agar, vermiculite, and coconut fiber) added to MS medium. In the rooting phase, different concentrations of indole butyric acid (IBA) (0.0, 0.5, 1.0, and 1.5 mg L-1) were added to culture medium containing the same substrate. Six months after the experiments were initiated, the survival percentage, number of leaves, shoots, and roots and length of the aerial part and the major root were evaluated. The results suggested that addition of 1.0 mg L-1 BAP is necessary for the O. baueri in vitro multiplication phase, but IBA is not necessary in the rooting phase. For the substrate, vermiculite is not indicated as an agar replacement. In contrast, coconut fiber can be used in both multiplication and rooting phases of Oncidium baueri in vitro culture.

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