Transcriptional regulation of human transcription factor IIB in SMMC-7721 human hepatocellular carcinoma cells by all- trans -retinoic acid and phorbol 12-myristate 13-acetate

1998 ◽  
Vol 124 (9) ◽  
pp. 493-496
Author(s):  
Chun Chen ◽  
Xianglei Yin ◽  
Dapeng Zhou ◽  
Zonghou Shen ◽  
Chengwu Chi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Retinoic Acid ◽  
Carcinoma Cells ◽  
Transcription Factor Iib ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Human Hepatocellular Carcinoma Cells ◽  
Human Transcription Factor

scholarly journals Intracellular alpha-fetoprotein interferes with all-trans retinoic acid induced ATG7 expression and autophagy in hepatocellular carcinoma cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shanshan Wang ◽  
Rilu Feng ◽  
Ying Shi ◽  
Dexi Chen ◽  
Honglei Weng ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Retinoic Acid ◽  
Carcinoma Cells ◽  
Alpha Fetoprotein ◽  
Cell Counting ◽  
All Trans Retinoic Acid ◽  
Induced Apoptosis ◽  
Anti Tumor Activity ◽  
Retinoid Acid ◽  
Hcc Cells

AbstractRetinoic acid and retinoid acid receptor (RA-RAR) signaling exhibits suppressive functions in the progression of hepatocellular carcinoma (HCC) through multiple mechanisms. However, whether RA-RAR signaling induces autophagy that contributes its anti-tumor activity in HCC remains elusive. In the current study, the effects of RA-RAR pathway on autophagy were investigated in two HCC cell lines: alpha-fetoprotein (AFP) positive PLC/PRF/5 and AFP negative HLE cells. Cell autophagy was analyzed with western blot for detection of LC3 conversion and p62/SQSTM1 degradation while autophagy flux was assayed using the mRFP-GFP-LC3 reporter. Cell apoptosis and viability were analyzed by caspase-3 activity, TdT-mediated dUTP nick end labeling (TUNEL) assay, and Cell Counting Kit (CCK)-8, respectively. Chromatin immunoprecipitation (ChIP) was employed to detect the binding of RAR onto the promoter of autophagy-relevant 7 (ATG7), and co-immunoprecipitation (CoIP) was used to analyze the interaction of AFP and RAR. The results showed that ATRA dosage and time-dependently induced high levels of cell autophagy in both the PLC/PRF/5 and HLE cells, which was accompanied with up-regulation of ATG7. ChIP assay showed that RAR was able to bind to its responsive elements on ATG7 promoter. Impairment of ATG7 induction or blockade of autophagy with chloroquine aggravated ATRA induced apoptosis of HCC cells. Furthermore, intracellular AFP was able to complex with RAR in PLC/PRF/5 cells. Knockdown of AFP in PLC/PRF/5 cells augmented the up-regulation of ATG7 by ATRA while overexpression of AFP in HLE cells attenuated ATRA induced ATG7 expression and autophagy. Thus, ATRA induced ATG7 and autophagy participated in its cytotoxicity on HCC cells and AFP interfere with the induction of ATG7 and autophagy through forming complex with RAR.

Transcriptional regulation of the homeobox geneNKX3.1 by all-trans retinoic acid in prostate cancer cells

2006 ◽  
Vol 99 (5) ◽  
pp. 1409-1419 ◽  
Author(s):  
Marc A. Thomas ◽  
Myles C. Hodgson ◽  
Susan D. Loermans ◽  
Joel Hooper ◽  
Raelene Endersby ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Transcriptional Regulation ◽  
Retinoic Acid ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid

Anticancer activity of all- trans retinoic acid-loaded liposomes on human thyroid carcinoma cells

2017 ◽  
Vol 150 ◽  
pp. 408-416 ◽  
Author(s):  
Maria Chiara Cristiano ◽  
Donato Cosco ◽  
Christian Celia ◽  
Andra Tudose ◽  
Rosario Mare ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Thyroid Carcinoma ◽  
Anticancer Activity ◽  
Carcinoma Cells ◽  
Human Thyroid ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid

Interaction of liposome-associated all-trans-retinoic acid with squamous carcinoma cells

1994 ◽  
Vol 34 (6) ◽  
pp. 527-534 ◽  
Author(s):  
Ranjani Parthasarathy ◽  
Peter G. Sacks ◽  
Daniel Harris ◽  
Heidi Brock ◽  
Kapil Mehta
Keyword(s):  
Retinoic Acid ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Squamous Carcinoma Cells ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid
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