Characterization of the cellular and humoral immune response to outer surface protein C and outer surface protein 17 in children with early disseminated Lyme borreliosis

2001 ◽  
Vol 189 (4) ◽  
pp. 193-200 ◽  
Author(s):  
Annette Pohl-Koppe ◽  
Angelika Kaunicnik ◽  
Bettina Wilske
Keyword(s):  
Immune Response ◽  
Lyme Borreliosis ◽  
Outer Surface ◽  
Humoral Immune Response ◽  
Protein C ◽  
Surface Protein ◽  
Humoral Immune ◽  
Outer Surface Protein

scholarly journals The Dominant Epitope of Borrelia garinii Outer Surface Protein C Recognized by Sera from Patients with Neuroborreliosis Has a Surface-Exposed Conserved Structural Motif

1998 ◽  
Vol 66 (9) ◽  
pp. 4073-4079 ◽  
Author(s):  
Marianne J. Mathiesen ◽  
Arne Holm ◽  
Michael Christiansen ◽  
Jens Blom ◽  
Klaus Hansen ◽  
...  
Keyword(s):  
B Cells ◽  
Lyme Borreliosis ◽  
Outer Surface ◽  
Protein C ◽  
Surface Protein ◽  
Terminal Region ◽  
Structural Motif ◽  
High Avidity ◽  
Binding Studies ◽  
Outer Surface Protein

ABSTRACT Epitope mapping of outer surface protein C (OspC) by using sera from patients with neuroborreliosis led to the identification of one single major immunodominant epitope within the C-terminal 10 amino acid residues. Peptide binding studies and alanine replacement scanning of the C-terminal decapeptide, PVVAESPKKP, revealed a critical role for the PKKP sequence and its terminal carboxyl group for the binding of immunoglobulin M (IgM) antibodies from patients with Lyme borreliosis. Electron microscopy of antibody-labeled spirochetes indicated that the C-terminal region is exposed on the surface of the spirochete. Based on homology to proteins of known function, this region most probably adopts a polyproline II-like helix, which is found in surface-exposed structures involved in protein-protein interactions. This structural motif is highly conserved in Borrelia species causing Lyme borreliosis and subjected to purifying selection. We suggest that the abundance of the C-terminal region of OspC on the surface of B. burgdorferi allows a multimeric high-avidity interaction between the spirochete and surface Igs on B cells. The resulting cross-linking of surface Igs on B cells may induce a T-cell-independent B-cell activation without IgM-to-IgG switching, thus explaining the lack of IgG antibodies to OspC in neuroborreliosis.

scholarly journals Inability of outer-surface protein C (OspC)-primed mice to elicit a protective anamnestic immune response to a tick-transmitted challenge of Borrelia burgdorferi

2003 ◽  
Vol 52 (7) ◽  
pp. 551-556 ◽  
Author(s):  
Robert D. Gilmore, Jr ◽  
Rendi M. Bacon ◽  
Amber M. Carpio ◽  
Joseph Piesman ◽  
Marc C. Dolan ◽  
...  
Keyword(s):  
Immune Response ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Protein C ◽  
Surface Protein ◽  
Outer Surface Protein

scholarly journals Persistent Anti- Borrelia IgM Antibodies without Lyme Borreliosis in the Clinical and Immunological Context

2021 ◽  
Vol 9 (3) ◽  
Author(s):  
Mateusz Markowicz ◽  
Michael Reiter ◽  
Jutta Gamper ◽  
Gerold Stanek ◽  
Hannes Stockinger
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Borreliosis ◽  
Outer Surface ◽  
Early Phase ◽  
Protein C ◽  
Surface Protein ◽  
Content Type ◽  
Igm Antibodies ◽  
Outer Surface Protein

The reactivity of human IgM with the outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is frequently used to detect Borrelia specific IgM in commercial immunoassays, and such antibodies usually occur in the early phase of the infection. We identified a group of individuals with persistent Borrelia IgM without symptoms of Lyme borreliosis.

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