Induction of apoptosis in human renal proximal tubular epithelial cells by Escherichia coli verocytotoxin 1 in vitro

1999 ◽  
Vol 188 (2) ◽  
pp. 73-78 ◽  
Author(s):  
Toshio Kodama ◽  
Kenichi Nagayama ◽  
Keiko Yamada ◽  
Yuichiro Ohba ◽  
Yukihiro Akeda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Tubular Epithelial Cells ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
Induction Of Apoptosis ◽  
Renal Proximal Tubular

scholarly journals No Cytotoxic and Inflammatory Effects of Empagliflozin and Dapagliflozin on Primary Renal Proximal Tubular Epithelial Cells under Diabetic Conditions In Vitro

2020 ◽  
Vol 21 (2) ◽  
pp. 391 ◽  
Author(s):  
Patrick C. Baer ◽  
Benjamin Koch ◽  
Janina Freitag ◽  
Ralf Schubert ◽  
Helmut Geiger
Keyword(s):  
Epithelial Cells ◽  
Cell Injury ◽  
Cellular Damage ◽  
Tubular Epithelial Cells ◽  
Urinary Glucose ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
Vitro Model ◽  
Renal Proximal Tubular

Gliflozins are inhibitors of the renal proximal tubular sodium-glucose co-transporter-2 (SGLT-2), that inhibit reabsorption of urinary glucose and they are able to reduce hyperglycemia in patients with type 2 diabetes. A renoprotective function of gliflozins has been proven in diabetic nephropathy, but harmful side effects on the kidney have also been described. In the current project, primary highly purified human renal proximal tubular epithelial cells (PTCs) have been shown to express functional SGLT-2, and were used as an in vitro model to study possible cellular damage induced by two therapeutically used gliflozins: empagliflozin and dapagliflozin. Cell viability, proliferation, and cytotoxicity assays revealed that neither empagliflozin nor dapagliflozin induce effects in PTCs cultured in a hyperglycemic environment, or in co-medication with ramipril or hydro-chloro-thiazide. Oxidative stress was significantly lowered by dapagliflozin but not by empagliflozin. No effect of either inhibitor could be detected on mRNA and protein expression of the pro-inflammatory cytokine interleukin-6 and the renal injury markers KIM-1 and NGAL. In conclusion, empa- and dapagliflozin in therapeutic concentrations were shown to induce no direct cell injury in cultured primary renal PTCs in hyperglycemic conditions.

Developing a self‐organized tubulogenesis model of human renal proximal tubular epithelial cells in vitro

2019 ◽  
Vol 108 (3) ◽  
pp. 795-804 ◽  
Author(s):  
Xiuli Wang ◽  
Chengchen Guo ◽  
Ying Chen ◽  
Lorenzo Tozzi ◽  
Sophia Szymkowiak ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tubular Epithelial Cells ◽  
Self Organized ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
Renal Proximal Tubular

Is there a role of cyclosporine A on total homocysteine export from human renal proximal tubular epithelial cells?

2001 ◽  
Vol 59 (s78) ◽  
pp. 258-261 ◽  
Author(s):  
Mihaela C. Ignatescu ◽  
Manuela Fodiger ◽  
Josef Kletzmayr ◽  
Christian Bieglmayer ◽  
Walter H. Horl ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cyclosporine A ◽  
Tubular Epithelial Cells ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
Total Homocysteine ◽  
Renal Proximal Tubular
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