Characteristics of a rat fibrosarcoma-derived transplantable tumour line (SS) and cultured cell lines (SS-P and SS-A3-1) showing myofibroblastic and histiocytic phenotypes

1997 ◽  
Vol 431 (6) ◽  
pp. 431-440 ◽  
Author(s):  
J. Yamate ◽  
M. Iwaki ◽  
Daijirou Kumagai ◽  
Yasuhiro Tsukamoto ◽  
Mitsuru Kuwamura ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cultured Cell ◽  
Transplantable Tumour

Lymphocytotoxicity inhibition: a reliable method for HL-A typing of cultured cell lines

1975 ◽  
Vol 14 (3-6) ◽  
pp. 241-246
Author(s):  
W.B. Bias ◽  
D.S. Borgaonkar ◽  
R.S. Kucherlapati ◽  
F.H. Ruddle
Keyword(s):  
Cell Lines ◽  
Reliable Method ◽  
Cultured Cell

scholarly journals Cultured Cell Sublines Highly Susceptible to Prion Infection

2000 ◽  
Vol 74 (9) ◽  
pp. 4377-4386 ◽  
Author(s):  
Patrick J. Bosque ◽  
Stanley B. Prusiner
Keyword(s):  
Cell Culture ◽  
Infected Cell ◽  
Tumor Cell ◽  
Cell Lines ◽  
Uninfected Cell ◽  
Resistant Cell ◽  
Cultured Cell ◽  
Prion Infection ◽  
Blot Technique ◽  
Prion Propagation

ABSTRACT Cultured cell lines infected with prions produce an abnormal isoform of the prion protein (PrPSc). In order to derive cell lines producing sufficient quantities of PrPSc for most studies, it has been necessary to subclone infected cultures and select the subclones producing the largest amounts of PrPSc. Since postinfection cloning can introduce differences between infected and uninfected cell lines, we sought an approach to generate prion-infected cell lines that would avoid clonal artifacts. Using an improved cell blot technique, which permits sensitive and rapid comparison of PrPSc levels in multiple independent cell cultures, we discovered marked heterogeneity with regard to prion susceptibility in tumor cell sublines. We exploited this heterogeneity to derive sublines which are highly susceptible to prion infection and used these cells to generate prion-infected lines without further subcloning. These infected sublines can be compared to the cognate uninfected cultures without interference from cloning artifacts. We also used susceptible cell lines and our modified cell blot procedure to develop a sensitive and reproducible quantitative cell culture bioassay for prions. We found that the sublines were at least 100-fold more susceptible to strain RML prions than to strain ME7 prions. Comparisons between scrapie-susceptible and -resistant cell lines may reveal factors that modulate prion propagation.

scholarly journals Establishment and characterization of two cultured cell lines derived from malignant rhabdoid tumors of the kidney

1996 ◽  
Vol 67 (2) ◽  
pp. 218-223 ◽  
Author(s):  
Masao Hirose ◽  
Tadashi Yamada ◽  
Tatsuo Abe ◽  
Takanori Hirose ◽  
Eiji Shimizu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cultured Cell ◽  
Rhabdoid Tumors

Comparison of the efficiency and toxicity of sonoporation with branched polyethylenimine-mediated gene transfection in various cultured cell lines

2008 ◽  
Vol 16 (10) ◽  
pp. 773-779 ◽  
Author(s):  
Chang S. Yoon ◽  
Hye S. Jung ◽  
Tae K. Kim ◽  
Min J. Kwon ◽  
Mi K. Kim ◽  
...  
Keyword(s):  
Cell Lines ◽  
Gene Transfection ◽  
Cultured Cell ◽  
Branched Polyethylenimine

PROGRESSIVE NEOPLASTIC GROWTH IN NUDE MICE OF CULTURED CELL LINES DERIVED FROM HUMAN GLIOMAS

1977 ◽  
Vol 36 (3) ◽  
pp. 593 ◽  
Author(s):  
D. D. Bigner ◽  
W. R. Markesberry ◽  
C. N. Pegram ◽  
B. Westermark ◽  
J. Pontén
Keyword(s):  
Cell Lines ◽  
Nude Mice ◽  
Human Gliomas ◽  
Neoplastic Growth ◽  
Cultured Cell
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