Induction of adenocarcinoma containing myoepithelial cells in rat submandibular gland by 7,12-dimethylbenz(a)anthracene

2000 ◽  
Vol 437 (3) ◽  
pp. 314-324 ◽  
Author(s):  
Y. Ogawa ◽  
F. Wan ◽  
Satoru Toyosawa ◽  
Naokuni Ijuhin
Keyword(s):  
Submandibular Gland ◽  
Myoepithelial Cells ◽  
Rat Submandibular Gland

scholarly journals Morphological Changes of Myoepithelial Cells in the Rat Submandibular Gland Following the Application of Surgical Stimuli

2016 ◽  
Vol 49 (6) ◽  
pp. 159-169 ◽  
Author(s):  
Yoshihiro Kawabe ◽  
Kenich Mizobe ◽  
Yasuhiko Bando ◽  
Koji Sakiyama ◽  
Fuyoko Taira ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Morphological Changes ◽  
Myoepithelial Cells ◽  
Rat Submandibular Gland

scholarly journals Fine structural localization of acetylcholinesterase activity in rat submandibular gland.

1985 ◽  
Vol 33 (5) ◽  
pp. 439-445 ◽  
Author(s):  
A Topilko ◽  
B Caillou
Keyword(s):  
Submandibular Gland ◽  
Ache Activity ◽  
Membrane System ◽  
Ultrastructural Localization ◽  
Acetylcholinesterase Activity ◽  
Myoepithelial Cells ◽  
Biologically Active ◽  
Intracytoplasmic Membrane ◽  
Structural Localization ◽  
Rat Submandibular Gland

Using the indirect thiocholine method, the ultrastructural localization of acetylcholinesterase (AChE) activity in the normal rat submandibular gland was studied. Cytochemical demonstration of AChE is based on coupling the hydrolysis of acetylthiocholine iodide to the precipitation of heavy metal salts. AChE-associated reaction product was selectively revealed in the perinuclear space and in the endoplasmic reticulum of the intercalated duct cells, in some cells of granular convoluted tubules, and in the striated duct epithelium, as well as in the myoepithelial cells. Although AChE activity generally occurred inside the cells, electron-dense precipitates were shown in intercellular space and in the stroma of the gland. Fine localization of AChE activity was also found in nerve bundles, predominantly between axons and between axons and Schwann cell. Our observations indicate that AChE is synthesized in the epithelium of the ducts and in the myoepithelial cells of the salivary gland. It is not known yet whether this enzyme is released from the intracytoplasmic membrane system into the extracellular space and then transported to the regions of the gland innervation. Conceivably AChE synthesized in the submandibular gland cells could also be considered an inhibitory modulator of the regulatory functions of biologically active polypeptides.

scholarly journals FINE STRUCTURAL LOCALIZATION OF CHOLINESTERASE ACTIVITY IN THE RAT SUBMANDIBULAR GLAND

1970 ◽  
Vol 18 (10) ◽  
pp. 730-739 ◽  
Author(s):  
BRUCE I. BOGART
Keyword(s):  
Smooth Muscle ◽  
Schwann Cell ◽  
Smooth Muscle Cells ◽  
Submandibular Gland ◽  
Muscle Cells ◽  
Acetylcholinesterase Activity ◽  
Myoepithelial Cells ◽  
Rat Submandibular Gland ◽  
Cell Interface ◽  
Axonal Swellings

The innervation of the rat submandibular gland was investigated by means of electron microscopy and cytochemical techniques. Axonal swellings partially depleted of their Schwann cell investment were observed in relationship to myoepithelial cells, acinar cells, ducts, capillaries and smooth muscle cells of arterioles. No direct contacts were found to exist between these elements and the nerve fibers. Axonal swellings containing mitochondria and vesicles fell into two distinct types: those characterized by small agranular vesicles and larger vesicles with pale cores and those characterized by small agranular vesicles and small vesicles with dense cores. Reaction product due to acetylcholinesterase activity was observed to be associated with the axolemma and the Schwann cell membrane at the axon-Schwann cell interface of many unmyelinated nerves found in relationship to both the vascular and parenchymal elements of the gland. Activity was found in association with the surface pits and vesicles of myoepithelial cells and arteriolar smooth muscle cells and with the rough endoplasmic reticulum and the nuclear envelope of the myoepithelial cells. Axonal swellings devoid of activity were observed particularly in relationship to arterioles. Reaction product was found only at the axon-Schwann cell interface when sections were incubated in medium for the demonstration of butyrylcholinesterase activity, while no reaction product was found upon addition of eserine sulfate to any of the media. The possibility that the reaction product due to acetylcholinesterase activity observed in association with the axolemma and the surface vesicles and pits of the myoepithelial cells and the arteriolar smooth muscle cells may define the autonomic cholinergic junction in the rat submandibular gland was explored.

Cytochemical Evidence for Presynatic β-Adrenergic Regulation of Secretion in the Developing Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 430-431
Author(s):  
L.S. Cutler
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Neonatal Rat ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Parotid Glands ◽  
Adrenergic Agonist ◽  
Rat Submandibular Gland

Many studies previously have shown that the B-adrenergic agonist isoproterenol and the a-adrenergic agonist norepinephrine will stimulate secretion by the adult rat submandibular (SMG) and parotid glands. Recent data from several laboratories indicates that adrenergic agonists bind to specific receptors on the secretory cell surface and stimulate membrane associated adenylate cyclase activity which generates cyclic AMP. The production of cyclic AMP apparently initiates a cascade of events which culminates in exocytosis. During recent studies in our laboratory it was observed that the adenylate cyclase activity in plasma membrane fractions derived from the prenatal and early neonatal rat submandibular gland was retractile to stimulation by isoproterenol but was stimulated by norepinephrine. In addition, in vitro secretion studies indicated that these prenatal and neonatal glands would not secrete peroxidase in response to isoproterenol but would secrete in response to norepinephrine. In contrast to these in vitro observations, it has been shown that the injection of isoproterenol into the living newborn rat results in secretion of peroxidase by the SMG (1).

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